The human serum proteome: Display of nearly 3700 chromatographically separated protein spots on two-dimensional electrophoresis gels and identification of 325 distinct proteins

PROTEOMICS ◽  
2003 ◽  
Vol 3 (7) ◽  
pp. 1345-1364 ◽  
Author(s):  
Rembert Pieper ◽  
Christine L. Gatlin ◽  
Anthony J. Makusky ◽  
Paul S. Russo ◽  
Courtney R. Schatz ◽  
...  
Keyword(s):  
Human Serum ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Serum Proteome ◽  
Dimensional Electrophoresis

Two-dimensional electrophoresis analysis of human serum proteins during the acute-phase response

1992 ◽  
Vol 13 (1) ◽  
pp. 743-746 ◽  
Author(s):  
Luca Bini ◽  
Barbara Magi ◽  
Carla Cellesi ◽  
Aldo Rossolini ◽  
Vitaliano Pallini
Keyword(s):  
Human Serum ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Serum Proteins ◽  
Phase Response ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis ◽  
Human Serum Proteins

The Two-Dimensional Electrophoresis of Human Serum DNA-Binding Proteins

1979 ◽  
Vol 7 (5) ◽  
pp. 1089-1090 ◽  
Author(s):  
ANNE E. HUGHES ◽  
W. SIDNEY B. LOWRY ◽  
J. ALAN HILL ◽  
R. JEREMY H. DAVIES
Keyword(s):  
Dna Binding ◽  
Human Serum ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Serum Dna ◽  
Dimensional Electrophoresis

Two-dimensional electrophoresis of human serum proteins modified by ampicillin during therapeutic treatment

1995 ◽  
Vol 16 (1) ◽  
pp. 1190-1192 ◽  
Author(s):  
Barbara Magi ◽  
Barbara Marzocchi ◽  
Luca Bini ◽  
Carla Cellesi ◽  
Aldo Rossolini ◽  
...  
Keyword(s):  
Human Serum ◽  
Serum Proteins ◽  
Two Dimensional ◽  
Therapeutic Treatment ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis ◽  
Human Serum Proteins

Microscale multisample two-dimensional electrophoresis of proteins in human serum, cerebrospinal fluid, and urine.

1982 ◽  
Vol 28 (4) ◽  
pp. 824-827 ◽  
Author(s):  
T Manabe ◽  
E Hayama ◽  
T Okuyama
Keyword(s):  
Cerebrospinal Fluid ◽  
Human Serum ◽  
Distribution Patterns ◽  
Blue Staining ◽  
Two Dimensional ◽  
Protein Distribution ◽  
Two Dimensional Electrophoresis ◽  
Coomassie Blue Staining ◽  
Coomassie Blue ◽  
Dimensional Electrophoresis

Abstract In this technique, in which no denaturing agent is used, proteins in human serum, cerebrospinal fluid, and urine are separated by isoelectric focusing in cylindrical 40 g/L polyacrylamide gels of capillary size (1.3 x 35 mm) for 40 min, followed by electrophoresis in 40--170 g/L polyacrylamide linear gradient gel, with use of 38 x 35 x 1 mm slab gel, for 1 h. Only 2 microL of untreated human serum is required to obtain clear protein-distribution patterns, made visible by Coomassie Blue staining. By use of silver staining, proteins in unconcentrated cerebrospinal fluid can be made visible. An apparatus we devised for microscale two-dimensional electrophoresis enables us to analyze eight protein samples simultaneously.

Sign in / Sign up

Export Citation Format

Share Document