scholarly journals An inhibitor of apoptosis (SfIAP) interacts with SQUAMOSA promoter-binding protein (SBP) transcription factors that exhibit pro-cell death characteristics

Plant Direct ◽  
2018 ◽  
Vol 2 (8) ◽  
pp. e00081 ◽  
Author(s):  
Ryan Kessens ◽  
Nick Sorensen ◽  
Mehdi Kabbage
Keyword(s):  
Cell Death ◽  
Transcription Factors ◽  
Binding Protein ◽  
Inhibitor Of Apoptosis ◽  
Squamosa Promoter Binding Protein

scholarly journals An inhibitor of apoptosis (SfIAP) interacts with SQUAMOSA promoter binding protein (SBP) transcription factors that exhibit pro-cell death characteristics

2018 ◽  
Author(s):  
Ryan Kessens ◽  
Nicholas Sorensen ◽  
Mehdi Kabbage
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Transcription Factors ◽  
Programmed Cell Death ◽  
Nuclear Localization ◽  
Binding Protein ◽  
Ectopic Expression ◽  
Inhibitor Of Apoptosis ◽  
Oxygen Species ◽  
Squamosa Promoter Binding Protein

AbstractDespite the functional conservation of programmed cell death (PCD) across broad evolutionary distances, an understanding of the molecular machinery underpinning this fundamental program in plants remains largely elusive. This is despite its critical importance to development, homeostasis, and proper responses to stress. Progress in plant PCD has been hindered by the fact that many core regulators of animal PCD are absent in plant genomes. Remarkably, numerous studies have shown that the ectopic expression of animal anti-PCD genes in plants can suppress cell death imposed by many stresses. In this study, we capitalize on the ectopic expression of an insect inhibitor of apoptosis (SfIAP) to identify novel cell death regulators in plants. A yeast two-hybrid assay was conducted using SfIAP as bait to screen a tomato cDNA library. This screen identified several transcription factors of the SQUAMOSA promoter binding protein (SBP) family as potential SfIAP binding partners. We confirmed this interaction in vivo for our top two interactors, SlySBP8b and SlySBP12a, using coimmunoprecipitation. Interestingly, overexpression of SlySBP8b and SlySBP12a induced spontaneous cell death in Nicotiana benthamiana leaves. Overexpression of these two transcription factors also induced the accumulation of reactive oxygen species and enhanced the growth of the necrotrophic pathogen Alternaria alternata. Fluorescence microscopy confirmed the nuclear localization of both SlySBP8b and SlySBP12a, while SlySBP12a was also localized to the ER membrane. These results support a pro-death role for SlySBP8b and SlySBP12a and provide potential targets that can be utilized to improve stress tolerance in crop plants.HighlightsSBP transcription factors SlySBP8b and SlySBP12a from tomato interact with an insect inhibitor of apoptosis protein (SfIAP). Both exhibit pro-cell death characteristics while SlySBP12a activity may be regulated through ER membrane tethering.AbbreviationsPCDprogrammed cell deathIAPinhibitor of apoptosisBIRbaculovirus IAP repeatRINGreally interesting new geneFB1fumonisin B1SBPSQUAMOSA promoter binding proteinROSreactive oxygen species35Scauliflower mosaic virus 35S promoterHAhemagglutininYFPyellow fluorescent proteinDAB3,3’-DiaminobenzidineQIS-Seqquantitative interactor screen sequencingCLSMconfocal laser scanning microscopyDHEdihydroethidiumNLSnuclear localization signalTMDtransmembrane domainERendoplasmic reticulumHRhypersensitive responseMTTFmembrane-tethered transcription factor

Apoptosis Inducing Factor Binding Protein PGAM5 Triggers Mitophagic Cell Death That Is Inhibited by the Ubiquitin Ligase Activity of X-Linked Inhibitor of Apoptosis

Biochemistry ◽  
2016 ◽  
Vol 55 (23) ◽  
pp. 3285-3302 ◽  
Author(s):  
Audrey M. Lenhausen ◽  
Amanda S. Wilkinson ◽  
Eric M. Lewis ◽  
Kaitlin M. Dailey ◽  
Andrew J. Scott ◽  
...  
Keyword(s):  
Cell Death ◽  
Ubiquitin Ligase ◽  
Binding Protein ◽  
Inhibitor Of Apoptosis ◽  
Factor Binding ◽  
Ubiquitin Ligase Activity ◽  
Apoptosis Inducing Factor

scholarly journals Genome-Wide Characterization and Expression Profiling of Squamosa Promoter Binding Protein-like (SBP) Transcription Factors in Wheat (Triticum aestivum L.)

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 527 ◽  
Author(s):  
Song ◽  
Ma ◽  
Yin ◽  
Yang ◽  
He ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Hexaploid Wheat ◽  
Abiotic Stresses ◽  
Binding Protein ◽  
Expression Patterns ◽  
Local Alignment ◽  
Composition Analysis ◽  
Motif Analysis ◽  
Genome Wide ◽  
Squamosa Promoter Binding Protein

Transcription factors (TFs) play fundamental roles in the developmental processes of all living organisms. Squamosa Promoter Binding Protein-like (SBP/SBP-Box) is a major family of plant-specific TFs, which plays important roles in multiple processes involving plant growth and development. While some work has been done, there is a lot more that is yet to be discovered in the hexaploid wheat SBP (TaSBP) family. With the completion of whole genome sequencing, genome-wide analysis of SBPs in common hexaploid wheat is now possible. In this study, we used protein–protein Basic Local Alignment Search Tool (BLASTp) to hunt the newly released reference genome sequence of hexaploid wheat (Chinese spring). Seventy-four TaSBP proteins (belonging to 56 genes) were identified and clustered into five groups. Gene structure and motif analysis indicated that most TaSBPs have relatively conserved exon–intron arrangements and motif composition. Analysis of transcriptional data showed that many TaSBP genes responded to some biological and abiotic stresses with different expression patterns. Moreover, three TaSBP genes were generally expressed in the majority of tissues throughout the wheat growth and also responded to many environmental biotic and abiotic stresses. Collectively, the detailed analyses presented here will help in understanding the roles of the TaSBP and also provide a reference for the further study of its biological function in wheat.

scholarly journals MIR156-Targeted SPL9 Is Phosphorylated by SnRK2s and Interacts With ABI5 to Enhance ABA Responses in Arabidopsis

2021 ◽  
Vol 12 ◽  
Author(s):  
Huixue Dong ◽  
Suli Yan ◽  
Yexing Jing ◽  
Ruizhen Yang ◽  
Yunwei Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Abscisic Acid ◽  
Protein Kinases ◽  
Plant Development ◽  
Binding Protein ◽  
Aba Signaling ◽  
Regulatory Module ◽  
Squamosa Promoter Binding Protein

The miR156-targeted SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors play key roles in regulating plant development, but little is known about their function in abscisic acid (ABA) signaling. Here, we report that the miR156-targeted SPLs enhance ABA responses and contribute to the inhibition of pre-harvest sprouting. We find that SPL9 directly activates the expression of ABA responsive genes through binding to their promoters. SPL9 was further shown to physically interact with ABSCISIC ACID INSENSITIVE 5 (ABI5), a master transcription factor in ABA signaling, thus promoting its association with the promoters of ABA responsive genes. Furthermore, we reveal that the protein kinases SnRK2s interact with and phosphorylate SPL9, which is essential for its role in the activation of ABA responses. Together, our results disclose a SnRK2s-SPLs-ABI5 regulatory module in ABA signaling in Arabidopsis.

scholarly journals The Impact of Anthocyanins and Iridoids on Transcription Factors Crucial for Lipid and Cholesterol Homeostasis

2021 ◽  
Vol 22 (11) ◽  
pp. 6074
Author(s):  
Maciej Danielewski ◽  
Agnieszka Matuszewska ◽  
Adam Szeląg ◽  
Tomasz Sozański
Keyword(s):  
Transcription Factors ◽  
Cholesterol Metabolism ◽  
Binding Protein ◽  
Regulatory Element ◽  
Cholesterol Homeostasis ◽  
Lipid Lowering ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Cell Functions ◽  
The Impact

Nutrition determines our health, both directly and indirectly. Consumed foods affect the functioning of individual organs as well as entire systems, e.g., the cardiovascular system. There are many different diets, but universal guidelines for proper nutrition are provided in the WHO healthy eating pyramid. According to the latest version, plant products should form the basis of our diet. Many groups of plant compounds with a beneficial effect on human health have been described. Such groups include anthocyanins and iridoids, for which it has been proven that their consumption may lead to, inter alia, antioxidant, cholesterol and lipid-lowering, anti-obesity and anti-diabetic effects. Transcription factors directly affect a number of parameters of cell functions and cellular metabolism. In the context of lipid and cholesterol metabolism, five particularly important transcription factors can be distinguished: liver X receptor (LXR), peroxisome proliferator-activated receptor-α (PPAR-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT/enhancer binding protein α (C/EBPα) and sterol regulatory element-binding protein 1c (SREBP-1c). Both anthocyanins and iridoids may alter the expression of these transcription factors. The aim of this review is to collect and systematize knowledge about the impact of anthocyanins and iridoids on transcription factors crucial for lipid and cholesterol homeostasis.

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