scholarly journals A metabolomics study of ascorbic acid‐induced in situ freezing tolerance in spinach ( Spinacia oleracea L.)

Plant Direct ◽  
2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Kyungwon Min ◽  
Keting Chen ◽  
Rajeev Arora
Keyword(s):  
Ascorbic Acid ◽  
Freezing Tolerance ◽  
Spinacia Oleracea ◽  
Spinacia Oleracea L ◽  
Metabolomics Study

l-ascorbic acid metabolism in spinach (Spinacia oleracea L.) during postharvest storage in light and dark

2003 ◽  
Vol 28 (1) ◽  
pp. 47-57 ◽  
Author(s):  
Merry Evelyn A Toledo ◽  
Yoshinori Ueda ◽  
Yoshihiro Imahori ◽  
Mitsuko Ayaki
Keyword(s):  
Ascorbic Acid ◽  
Acid Metabolism ◽  
Spinacia Oleracea ◽  
Postharvest Storage ◽  
Spinacia Oleracea L

scholarly journals OPTIMAL POSTHARVEST STORAGE PARAMETERS AND SHELF LIFE OF BABY SPINACH (SPINACIA OLERACEA L.)

2015 ◽  
Author(s):  
Jonas VIŠKELIS ◽  
Marina RUBINSKIENĖ ◽  
Dalia URBONAVIČIENĖ ◽  
Ramunė BOBINAITĖ, ◽  
Pranas VIŠKELIS,
Keyword(s):  
Ascorbic Acid ◽  
Shelf Life ◽  
Storage Temperature ◽  
Spinacia Oleracea ◽  
Total Content ◽  
Visual Quality ◽  
Soluble Solids ◽  
Postharvest Storage ◽  
Spinacia Oleracea L ◽  
Biochemical Quality

This work was done to evaluate the optimal postharvest storage parameters and shelf life of baby spinach. Baby spinach (50 g) was sealed in three different polypropylene (PP) and polyethylene (PE) packaging materials: 30 m (PP), 35 m (PE) & 40 m (PE), and were stored in darkness for 3 and 9 days at 0 °C, 4 °C, 8 °C & 16 °C ( 1 °C). Total content of phenolics, soluble solids, nitrates, chlorophyll content, ascorbic acid & spinach color (CIEL*a*b*) were measured by standard methods after 3 and 9 days of storage. Baby spinach stored at 0  1 °C and 4  1 °C showed the best visual quality. Storage temperature had significant influence on spinach colour, but the influence of packaging was not so strong. The content of soluble solids, ascorbic acid and nitrates decreased during storage. The highest amount of ascorbic acid was retained when baby spinach were stored in 40 m PE bags at 0  1 °C. When baby spinach was stored at higher temperatures (4 °C, 8 °C & 16 °C), the decrease in the amount of ascorbic acid ranged from 55 % to 69 %. Baby spinach stored for 9 days at 0 °C & 4 °C showed high visual and biochemical quality. Higher amounts of soluble solids and ascorbic acid were found in spinach stored in 40 m PE bags. Keywords: postharvest storage, spinach, Spinacia oleracea L.

scholarly journals A metabolomics study of ascorbic acid-induced in situ freezing tolerance in spinach (Spinacia oleracea L.)

2020 ◽  
Author(s):  
Kyungwon Min ◽  
Keting Chen ◽  
Rajeev Arora
Keyword(s):  
Ascorbic Acid ◽  
Freezing Tolerance ◽  
Spinacia Oleracea ◽  
Leaf Growth ◽  
Compatible Solutes ◽  
Ion Leakage ◽  
Plant Tolerance ◽  
Freeze Thaw ◽  
Exogenous Application ◽  
Leaf Level

AbstractFreeze-thaw stress is one of the major environmental constraints that limit plant growth and reduces productivity and quality. Plants exhibit a variety of cellular dysfunction following freeze-thaw stress, including accumulation of reactive oxygen species (ROS). This means that enhancement of antioxidant capacity by exogenous application of antioxidants could potentially be one of major strategies for improving freezing tolerance (FT) of plants. Exogenous application of ascorbic acid (AsA), as an antioxidant, has been shown to improve plant tolerance against abiotic stresses but its effect on FT has not been investigated. We evaluated the effect of AsA-feeding on FT of spinach (Spinacia oleracea L.) at whole-plant and excised leaf level, and conducted metabolite profiling of leaves before and after AsA-treatment to explore metabolic explanation for change in FT. AsA-application did not impede leaf-growth; instead slightly promoted it. Temperature-controlled freeze-thaw tests revealed AsA-fed plants were more freezing tolerant as indicated by: 1) less visual damage/mortality; 2) lower ion-leakage; and 3) less oxidative-injury, lower abundance of free radicals (O2•− and H2O2). Comparative leaf metabolic profiling revealed clear separation of metabolic phenotypes for control vs. AsA-fed leaves. Specifically, AsA-fed leaves had greater abundance of antioxidants (AsA, glutathione, alpha-& gamma-tocopherol) and compatible solutes (proline, galactinol, myo-inositol). AsA-fed leaves also had higher activity of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, catalase). These changes, together, may improve FT via alleviating freeze-induced oxidative stress as well as protecting membranes from freeze-desiccation. Additionally, improved FT by AsA-feeding may potentially include enhanced cell wall/lignin augmentation and bolstered secondary metabolism as indicated by diminished level of phenylalanine and increased abundance of branched amino acids, respectively.

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