scholarly journals Identification of functional single nucleotide polymorphism of Populus trichocarpa PtrEPSP‐TF and determination of its transcriptional effect

Plant Direct ◽  
2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Meng Xie ◽  
Jin Zhang ◽  
Vasanth R. Singan ◽  
Melissa J. McGranahan ◽  
Peter R. LaFayette ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Populus Trichocarpa ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Transcriptional Effect ◽  
Functional Single Nucleotide Polymorphism

Simultaneous determination of Mycobacterium leprae drug resistance and single nucleotide polymorphism genotype using nested multiplex PCR with amplicon sequencing

2021 ◽  
Author(s):  
Yasuhisa Iwao ◽  
Shuichi Mori ◽  
Manabu Ato ◽  
Noboru Nakata
Keyword(s):  
Drug Resistance ◽  
Single Nucleotide Polymorphism ◽  
Multiplex Pcr ◽  
Simultaneous Determination ◽  
Mycobacterium Leprae ◽  
Amplicon Sequencing ◽  
Clinical Samples ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide

Mycobacterium leprae is the predominant cause of leprosy worldwide, and its genotypes can be classified into four single nucleotide polymorphism (SNP) types and 16 subtypes. Determining M. leprae drug resistance and genotype is typically done by PCR and Sanger DNA sequencing, which require substantial effort. Here we describe a rapid method involving multiplex PCR in combination with nested amplification and next generation sequence analysis that allows simultaneous determination of M. leprae drug resistance and SNP genotype directly from clinical specimens. We used this method to analyze clinical samples from two paucibacillary, nine multibacillary, and six type-undetermined leprosy patients. Regions in folP1 , rpoB , gyrA , and gyrB that determine drug resistance and those for 84 SNP-InDels in the M. leprae genome were amplified from clinical samples and their sequences were determined. The results showed that seven samples were subtype 1A, three were 1D, and seven were 3K. Three samples of the subtype 3K had folp1 mutation. The method may allow more rapid genetic analyses of M. leprae in clinical samples.

scholarly journals Determination of Residual Feed Intake and Its Associations with Single Nucleotide Polymorphism in Chickens

2014 ◽  
Vol 13 (1) ◽  
pp. 148-157 ◽  
Author(s):  
Zhen-qiang XU ◽  
Jie CHEN ◽  
Yan ZHANG ◽  
Cong-liang JI ◽  
De-xiang ZHANG ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Feed Intake ◽  
Residual Feed Intake ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide
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