Studies of the antenna effect in polymer molecules V. Determination of the extent of singlet energy migration in a phenanthrene — Containing polymer by fluorescence quenching

1982 ◽  
Vol 14 (4) ◽  
pp. 159-162 ◽  
Author(s):  
David A. Holden ◽  
Dominic Ng ◽  
James E. Guillet
Keyword(s):  
Fluorescence Quenching ◽  
Energy Migration ◽  
Antenna Effect ◽  
Polymer Molecules

A Homogeneous Nonisotopic Histone Deacetylase Activity Assay

2003 ◽  
Vol 8 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Birgit Heltweg ◽  
Manfred Jung
Keyword(s):  
Drug Discovery ◽  
Fluorescence Quenching ◽  
Histone Deacetylase ◽  
Anticancer Agents ◽  
Histone Deacetylases ◽  
Activity Assay ◽  
Animal Testing ◽  
Hdac Activity

Histone deacetylases (HDACs) are important regulators of transcription, and their inhibitors are a promising class of anticancer agents. The methods for the determination of HDAC activity and its inhibition that are currently available suffer from various drawbacks, such as animal testing, radioactive substrates, or limited throughput. Therefore, a fast nonisotopic method for the measurement of HDAC activity is highly desirable. The authors present such an assay that relies on the fluorescent HDAC substrate developed previously in their group. After incubation of the substrate with the enzyme, a derivatization leads to efficient fluorescence quenching in the deacetylated metabolite. Thus, only the fluorescence emitted by the remaining substrate is detected, which allows for a convenient detection of HDAC activity in a homogeneous format that can be performed on multiwell plate readers. This procedure, called HDASH (histone deacetylase assay—homogeneous), should be a valuable tool in transcriptional research and especially drug discovery. ( Journal of Biomolecular Screening 2003:89-95)

Determination of Sodium Hexametaphosphate in Beverages Based on the Fluorescence Quenching of Acridine Orange

2014 ◽  
Vol 47 (16) ◽  
pp. 2740-2746
Author(s):  
Heng Xin Zhao ◽  
Ming Luo ◽  
Li Xin Mo ◽  
Liang Cheng ◽  
Zheng Ma ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Acridine Orange ◽  
Sodium Hexametaphosphate

Determination of Concentration of Amphiphilic Polymer Molecules on the Surface of Encapsulated Semiconductor Nanocrystals

Langmuir ◽  
2016 ◽  
Vol 32 (8) ◽  
pp. 1955-1961 ◽  
Author(s):  
Aleksandra Fedosyuk ◽  
Aliaksandra Radchanka ◽  
Artsiom Antanovich ◽  
Anatol Prudnikau ◽  
Maksim V. Kvach ◽  
...  
Keyword(s):  
Semiconductor Nanocrystals ◽  
Amphiphilic Polymer ◽  
Polymer Molecules
Sign in / Sign up

Export Citation Format

Share Document