Impact of an Immunoglobulin G-Specific Enzyme-Linked Immunosorbent Assay on the Management of Heparin-Induced Thrombocytopenia

2013 ◽  
Vol 33 (11) ◽  
pp. 1191-1198 ◽  
Author(s):  
Katelyn W. Sylvester ◽  
John Fanikos ◽  
Kevin E. Anger ◽  
Lina Matta ◽  
Robert A. Southard ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Enzyme ◽  
Heparin Induced Thrombocytopenia

Immunoglobulin G- and M-specific enzyme-linked immunosorbent assay for detection of dengue antibodies

1979 ◽  
Vol 9 (4) ◽  
pp. 498-502
Author(s):  
D Dittmar ◽  
T J Cleary ◽  
A Castro
Keyword(s):  
Dengue Virus ◽  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Hemagglutination Inhibition ◽  
Dengue Infection ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Enzyme ◽  
Enzyme Conjugate ◽  
Inhibition Technique

An enzyme-linked immunosorbent assay for the detection of antibodies to dengue virus is described. This method correlates well with a hemagglutination inhibition technique. The enzyme-linked immunosorbent assay can also be specific for human immunoglobulin M antibodies when a mu-chain-specific antiglobulin-enzyme conjugate and fractionated serum are employed. By using this technique, dengue immunoglobulin M antibodies were demonstrated in an infant suspected of having a recent dengue infection.

scholarly journals Evaluation of the INOVA Diagnostics Enzyme-Linked Immunosorbent Assay Kits for Measuring Serum Immunoglobulin G (IgG) and IgA to Deamidated Gliadin Peptides

2006 ◽  
Vol 13 (1) ◽  
pp. 150-151 ◽  
Author(s):  
Harry E. Prince
Keyword(s):  
Celiac Disease ◽  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Immunoglobulin A ◽  
Serum Immunoglobulin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gliadin Antibodies ◽  
Gliadin Peptides ◽  
Disease Specific ◽  
Inova Diagnostics

ABSTRACT New assays for antibodies to deamidated gliadin peptides (DGP) expressing celiac disease-specific epitopes were evaluated using 154 sera previously tested for endomysial immunoglobulin A (IgA) (EMA), transglutaminase IgA (TGA), and conventional gliadin antibodies. DGP antibody results showed 97% concordance with EMA and TGA results. Of 56 sera negative for EMA and TGA but positive for conventional gliadin antibodies, 54 (96%) were negative for DGP antibodies.

scholarly journals Analysis of Specific Immunoglobulin G Subclass Antibodies for Serological Diagnosis of Echinococcosis by a Standard Enzyme-Linked Immunosorbent Assay

1998 ◽  
Vol 5 (5) ◽  
pp. 613-616 ◽  
Author(s):  
Felix Grimm ◽  
Friedrich E. Maly ◽  
Jian Lü ◽  
Roberto Llano
Keyword(s):  
Healthy Volunteers ◽  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Parasitic Infections ◽  
Igg Subclass ◽  
Specific Antibodies ◽  
Specific Igg4 ◽  
Antibody Levels

ABSTRACT The potential roles of specific antibodies of the different immunoglobulin G (IgG) subclasses in the serological diagnosis of cystic echinococcosis (CE) and alveolar echinococcosis (AE) were investigated by an enzyme-linked immunosorbent assay based on hydatid fluid as antigen. Specific antibodies of subclass 1 were found to be of major importance. In sera collected at the time of diagnosis (i.e., before any therapeutic intervention was initiated) they could be demonstrated in 14 of 15 sera from patients with CE and in all 12 sera from patients with AE. The most discriminatory and the most specific antibodies found in this study belonged to IgG subclass 4. Only one false-positive reaction was observed with 253 sera from healthy volunteers, and no cross-reactions occurred in 80 sera from patients with different parasitic infections. Specific IgG4 antibodies could be demonstrated in 61.0 to 66.7% (CE) or 47.6 to 66.7% (AE) of the cases. Antibody levels of IgG subclass 2 were elevated only moderately, and subclass 3 antibodies were detected in a few cases only. In addition, nonspecific reactions in sera of healthy volunteers or patients with other parasitic infections could partially be attributed to antibodies of subclasses 2 and 3.

scholarly journals Hemagglutinin-specific enzyme-linked immunosorbent assay for antibodies to influenza A and B viruses.

1981 ◽  
Vol 13 (3) ◽  
pp. 554-560 ◽  
Author(s):  
B R Murphy ◽  
M A Phelan ◽  
D L Nelson ◽  
R Yarchoan ◽  
E L Tierney ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Influenza A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Enzyme

scholarly journals Idiotypes of anti-Ia antibodies. I. Expression of the 14-4-4S idiotype in humoral immune responses.

1981 ◽  
Vol 154 (2) ◽  
pp. 397-409 ◽  
Author(s):  
S L Epstein ◽  
K Ozato ◽  
J A Bluestone ◽  
D H Sachs
Keyword(s):  
Immune Responses ◽  
Immunosorbent Assay ◽  
Heavy Chain ◽  
Antigenic Specificity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Absorption Analysis ◽  
Specific Enzyme ◽  
Cellular Receptors ◽  
Humoral Immune ◽  
Humoral Immune Responses

The idiotype of a mouse monoclonal anti-I-E antibody, 14-4-4S, has been studied using a heterologous anti-idiotypic reagent. This antibody recognizes Ia. 7, an antigenic specificity present in all strains expressing a product of the I-E subregion. Expression of the 14-4-4S idiotype in humoral immune responses was analyzed by an idiotype-specific enzyme-linked immunosorbent assay system. The idiotype was readily detectable in C3H.SW anti-C3H alloantisera, the same immunization combination from which the hybridoma was derived. Absorption analysis demonstrated the anti-I-E specificity of the idiotype-positive molecules in these alloantisera. Penetrance of idiotype expression was high among individual C3H.SW immune mice (9 of 10 tested). To examine genetic requirements for idiotype expression, an immunization was performed using as responders CWB mice, congenic with C3H.SW but differing at the heavy chain allotype loci. Immune sera of individual CWB mice contained very little or no idiotype, demonstrating that levels of idiotype expression are influenced by allotype-linked genes, although the influence of other genes has not been ruled. The 14-4-4S idiotype therefore represents a shared idiotype of anti-Ia antibodies and provides opportunities for analysis of the idiotypes of cellular receptors for the corresponding Ia antigen.

Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms

1981 ◽  
Vol 68 (2) ◽  
pp. 112-118 ◽  
Author(s):  
J.Andrew Grant ◽  
Randall M. Goldblum ◽  
Richard Rahr ◽  
David O. Thueson ◽  
Jafar Farnam ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Enzyme Linked Immunosorbent Assay
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