Effects of monomer composition on physical properties and enzymatic hydrolyzability of poly(butylene succinate‐ co ‐ hexamethylene succinate)s

Author(s):  
Jing Jing ◽  
Li Song ◽  
Tingting Su ◽  
Zhanyong Wang
2008 ◽  
Vol 93 (5) ◽  
pp. 889-895 ◽  
Author(s):  
Sang-Il Han ◽  
Jung Seop Lim ◽  
Dong Kook Kim ◽  
Mal Nam Kim ◽  
Seung Soon Im

2000 ◽  
Vol 66 (4) ◽  
pp. 1311-1320 ◽  
Author(s):  
Qun Ren ◽  
Nicolas Sierro ◽  
Michele Kellerhals ◽  
Birgit Kessler ◽  
Bernard Witholt

ABSTRACT To prepare medium-chain-length poly-3-hydroxyalkanoates (PHAs) with altered physical properties, we generated recombinant Escherichia coli strains that synthesized PHAs with altered monomer compositions. Experiments with different substrates (fatty acids with different chain lengths) or different E. coli hosts failed to produce PHAs with altered physical properties. Therefore, we engineered a new potential PHA synthetic pathway, in which ketoacyl-coenzyme A (CoA) intermediates derived from the β-oxidation cycle are accumulated and led to the PHA polymerase precursorR-3-hydroxyalkanoates in E. coli hosts. By introducing the poly-3-hydroxybutyrate acetoacetyl-CoA reductase (PhbB) from Ralstonia eutropha and blocking the ketoacyl-CoA degradation step of the β-oxidation, the ketoacyl-CoA intermediate was accumulated and reduced to the PHA precursor. Introduction of thephbB gene not only caused significant changes in the monomer composition but also caused changes of the physical properties of the PHA, such as increase of polymer size and loss of the melting point. The present study demonstrates that pathway engineering can be a useful approach for producing PHAs with engineered physical properties.


2001 ◽  
Vol 57 (6) ◽  
pp. 165-171 ◽  
Author(s):  
Takahiro Uesaka ◽  
Nobuo Ogata ◽  
Koji Nakane ◽  
Takashi Ogihara

1992 ◽  
Vol 8 (2) ◽  
pp. 137-139 ◽  
Author(s):  
K. Yoshida ◽  
H. Matsumura ◽  
T. Tanaka ◽  
M. Atsuta

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