Fetal nucleated red blood cells in peripheral blood of pregnant women: detection and determination of location on a slide using laser-scanning cytometry

2003 ◽  
Vol 23 (9) ◽  
pp. 710-715 ◽  
Author(s):  
Simone Hennerbichler ◽  
Peter M Kroisel ◽  
Hannelore Zierler ◽  
Barbara Pertl ◽  
Reinhold Wintersteiger ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Pregnant Women ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Laser Scanning ◽  
Nucleated Red Blood Cells ◽  
Laser Scanning Cytometry

Detection and relocation of cord blood nucleated red blood cells by laser scanning cytometry

Cytometry ◽  
2002 ◽  
Vol 48 (2) ◽  
pp. 87-92 ◽  
Author(s):  
Simone Hennerbichler ◽  
Rudolf Schmied ◽  
Erwin Petek ◽  
Peter M. Kroisel ◽  
Barbara Pertl ◽  
...  
Keyword(s):  
Cord Blood ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Laser Scanning ◽  
Nucleated Red Blood Cells ◽  
Laser Scanning Cytometry

scholarly journals A microfluidics approach for the isolation of nucleated red blood cells (NRBCs) from the peripheral blood of pregnant women

2008 ◽  
Vol 28 (10) ◽  
pp. 892-899 ◽  
Author(s):  
R. Huang ◽  
T. A. Barber ◽  
M. A. Schmidt ◽  
R. G. Tompkins ◽  
M. Toner ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Pregnant Women ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Nucleated Red Blood Cells

scholarly journals A Silicon-based Coral-like Nanostructured Microfluidics to Isolate Rare Cells in Human Circulation: Validation by SK-BR-3 Cancer Cell Line and Its Utility in Circulating Fetal Nucleated Red Blood Cells

Micromachines ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 132 ◽  
Author(s):  
Gwo-Chin Ma ◽  
Wen-Hsiang Lin ◽  
Chung-Er Huang ◽  
Ting-Yu Chang ◽  
Jia-Yun Liu ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Pregnant Women ◽  
Blood Cells ◽  
De Novo ◽  
Capture Rate ◽  
Genetic Diagnosis ◽  
Maternal Blood ◽  
Capture Efficiency ◽  
Nucleated Red Blood Cells ◽  
Silicon Based

Circulating fetal cells (CFCs) in maternal blood are rare but have a strong potential to be the target for noninvasive prenatal diagnosis (NIPD). “Cell RevealTM system” is a silicon-based microfluidic platform capable to capture rare cell populations in human circulation. The platform is recently optimized to enhance the capture efficiency and system automation. In this study, spiking tests of SK-BR-3 breast cancer cells were used for the evaluation of capture efficiency. Then, peripheral bloods from 14 pregnant women whose fetuses have evidenced non-maternal genomic markers (e.g., de novo pathogenic copy number changes) were tested for the capture of circulating fetal nucleated red blood cells (fnRBCs). Captured cells were subjected to fluorescent in situ hybridization (FISH) on chip or recovered by an automated cell picker for molecular genetic analyses. The capture rate for the spiking tests is estimated as 88.1%. For the prenatal study, 2–71 fnRBCs were successfully captured from 2 mL of maternal blood in all pregnant women. The captured fnRBCs were verified to be from fetal origin. Our results demonstrated that the Cell RevealTM system has a high capture efficiency and can be used for fnRBC capture that is feasible for the genetic diagnosis of fetuses without invasive procedures.

Poor prognosis indicated by nucleated red blood cells in peripheral blood is not associated with organ failure of the liver or kidney

2006 ◽  
Vol 44 (8) ◽  
Author(s):  
Axel Stachon ◽  
Tim Holland-Letz ◽  
Reiner Kempf ◽  
Andreas Becker ◽  
Jochen Friese ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Organ Failure ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Poor Prognosis ◽  
Nucleated Red Blood Cells

AbstractClin Chem Lab Med 2006;44:955–61.

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