Copy number analysis of meiotic and postzygotic mitotic aneuploidies in trophectoderm cells biopsied at the blastocyst stage and arrested embryos

Monoclonal antibodies were raised to detergent-extracted cytoskeleton preparations of mouse oocytes. In immunofluorescence microscopy, one of the antibodies, OCS-1, localizes exclusively to epithelial cells in frozen tissue sections, including various simple and stratified epithelia. The antibody decorates a keratin-type of fibrillax, vinblastine-resistant network in various cultured, epithelial-type cells, but not in myoid or fibroblastoid cells. In mouse oocytes and cleavage-stage embryos, the OCS-1 antibody gives a diffuse, spotty staining pattern. In blastocyst-stage embryos, the antibody reveals a keratin-type filamentous organization in the trophectoderm cells. In immunoelectron microscopy, the OCS-1 antibody decorates 10 nm-thick filaments, often identifiable as desmosome-attached tonofilaments, in detergent-treated trophectoderm cells. The antigen(s) recognized by the OCS-1 antibody is apparently present in, or closely associated with, cytokeratin filaments. In addition to mouse oocytes and early embryos, a wide variety of epithelial cells in various species seem to share this antigen(s). The present results suggest that at the early stages, the cytokeratin-related antigen(s) defined by the OCS-1 antibody are stored in a non-fibrillar form which is then converted into a fibrillar network at the blastocyst stage. A pre-existing supply of cytokeratin-related protein may be essential for the development of the blastocyst.

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Localization of cytoskeletal proteins in preimplantation mouse embryos

Development ◽

10.1242/dev.55.1.211 ◽

1980 ◽

Vol 55 (1) ◽

pp. 211-225

Author(s):

E. Lehtonen ◽

R. A. Badley

Keyword(s):

Blastocyst Stage ◽

Cytoskeletal Proteins ◽

Mouse Embryos ◽

Trophectoderm Cells ◽

Apparent Concentration ◽

Preimplantation Mouse ◽

Early Mouse ◽

Filament Protein

The immunofluorescence technique was used to detect the presence and distribution of actin, alpha-actinin, tubulin and 10 nm filament protein in early mouse embryos. Actin and alpha-actinin stainings showed a distinct concentration to a peripheral layer in the cleavage-stage blastomeres and in trophectoderm cells. Dots of fluorescence appeared in this cortical staining pattern. The distribution of tubulin staining in the blastomere cytoplasm was relatively even with apparent concentration at the perinuclear region and frequently at wide intercellular contact areas. 10 nm filament protein was distributed evenly in the blastomere cytoplasm without cortical concentration of the label. At the blastocyst stage, the trophectoderm cells in blastocyst outgrowths in vitro developed well organized cytoskeletons including both microfilament, microtubule and 10 nm filament elements. Comparable structures were not observed in blastocysts in vivo, or in late hatched blastocysts cultured in suspension. The morphogenetic significance of the observations is discussed.

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Copy number analysis by low coverage whole genome sequencing using ultra low-input DNA from formalin-fixed paraffin embedded tumor tissue

Genome Medicine ◽

10.1186/s13073-016-0375-z ◽

2016 ◽

Vol 8 (1) ◽

Cited By ~ 18

Author(s):

Tanjina Kader ◽

David L. Goode ◽

Stephen Q. Wong ◽

Jacquie Connaughton ◽

Simone M. Rowley ◽

...

Keyword(s):

Whole Genome Sequencing ◽

Genome Sequencing ◽

Copy Number ◽

Tumor Tissue ◽

Whole Genome ◽

Copy Number Analysis ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Low Coverage ◽

Formalin Fixed

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Bayesian DNA copy number analysis

BMC Bioinformatics ◽

10.1186/1471-2105-10-10 ◽

2009 ◽

Vol 10 (1) ◽

Cited By ~ 24

Author(s):

Paola MV Rancoita ◽

Marcus Hutter ◽

Francesco Bertoni ◽

Ivo Kwee

Keyword(s):

Copy Number ◽

Copy Number Analysis ◽

Dna Copy Number

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