Replicate real-time PCR testing of DNA in maternal plasma increases the sensitivity of non-invasive fetal sex determination

2003 ◽  
Vol 23 (3) ◽  
pp. 235-238 ◽  
Author(s):  
Ilona Hromadnikova ◽  
Bela Houbova ◽  
Dana Hridelova ◽  
Sona Voslarova ◽  
Josef Kofer ◽  
...  
Keyword(s):  
Sex Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Maternal Plasma ◽  
Fetal Sex ◽  
Non Invasive

Application of real-time PCR of sex-independent insertion-deletion polymorphisms to determine fetal sex using cell-free fetal DNA from maternal plasma

2015 ◽  
Vol 53 (8) ◽  
Author(s):  
Sherry Sze Yee Ho ◽  
Angela Barrett ◽  
Henna Thadani ◽  
Cecille Laureano Asibal ◽  
Evelyn Siew-Chuan Koay ◽  
...  
Keyword(s):  
Real Time ◽  
Y Chromosome ◽  
Real Time Pcr ◽  
Genetic Material ◽  
Maternal Plasma ◽  
Fetal Sex ◽  
Female Fetus ◽  
Non Invasive ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

AbstractPrenatal diagnosis of sex-linked disorders requires invasive procedures, carrying a risk of miscarriage of up to 1%. Cell-free fetal DNA (cffDNA) present in cell-free DNA (cfDNA) from maternal plasma offers a non-invasive source of fetal genetic material for analysis. Detection of Y-chromosome sequences in cfDNA indicates presence of a male fetus; in the absence of a Y-chromosome signal a female fetus is inferred. We aimed to validate the clinical utility of insertion-deletion polymorphisms (INDELs) to confirm presence of a female fetus using cffDNA.Quantitative real-time PCR (qPCR) for the Y-chromosome-specific sequence,Fetal sex was correctly determined in 77/82 (93.9%) cfDNA samples.We have validated a non-invasive prenatal test to confirm fetal sex as early as 6 gestational weeks using cffDNA from maternal plasma.

scholarly journals Non-Invasive Fetal Sex Determination Using SRY Specific Primers and Sybrgreen Real Time PCR

2011 ◽  
Vol 11 (2) ◽  
Author(s):  
I. SvecovA ◽  
E. Jezkova ◽  
I Hudecova ◽  
T. Burjanivova ◽  
K. Biskupska-Bodova ◽  
...  
Keyword(s):  
Sex Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Specific Primers ◽  
Fetal Sex ◽  
Non Invasive

Non-invasive fetal sex determination using real-time PCR

2006 ◽  
Vol 19 (6) ◽  
pp. 337-342 ◽  
Author(s):  
Katarina Davalieva ◽  
Pavle Dimcev ◽  
Georgi D. Efremov ◽  
Dijana Plaseska-Karanfilska
Keyword(s):  
Sex Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Fetal Sex ◽  
Non Invasive

scholarly journals Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma

Diagnostics ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 564
Author(s):  
Jana Bohmova ◽  
Marek Lubusky ◽  
Iva Holuskova ◽  
Martina Studnickova ◽  
Romana Kratochvilova ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Real Time ◽  
Real Time Pcr ◽  
Dna Analysis ◽  
Methodological Approach ◽  
Maternal Plasma ◽  
Buccal Swab ◽  
Non Invasive ◽  
Pcr Method ◽  
Parallel Reactions

Noninvasive fetal RHD genotyping is an important tool for predicting RhD incompatibility between a pregnant woman and a fetus. This study aimed to assess a methodological approach other than the commonly used one for noninvasive fetal RHD genotyping on a representative set of RhD-negative pregnant women. The methodology must be accurate, reliable, and broadly available for implementation into routine clinical practice. A total of 337 RhD-negative pregnant women from the Czech Republic region were tested in this study. The fetal RHD genotype was assessed using two methods: real-time PCR and endpoint quantitative fluorescent (QF) PCR. We used exon-7-specific primers from the RHD gene, along with internal controls. Plasma samples were analyzed and measured in four/two parallel reactions to determine the accuracy of the RHD genotyping. The RHD genotype was verified using DNA analysis from a newborn buccal swab. Both methods showed an excellent ability to predict the RHD genotype. Real-time PCR achieved its greatest accuracy of 98.6% (97.1% sensitivity and 100% specificity (95% CI)) if all four PCRs were positive/negative. The QF PCR method also achieved its greatest accuracy of 99.4% (100% sensitivity and 98.6% specificity (95% CI)) if all the measurements were positive/negative. Both real-time PCR and QF PCR were reliable methods for precisely assessing the fetal RHD allele from the plasma of RhD-negative pregnant women.

First-trimester fetal sex determination in maternal serum using real-time PCR

2001 ◽  
Vol 21 (12) ◽  
pp. 1070-1074 ◽  
Author(s):  
Jean-Marc Costa ◽  
Alexandra Benachi ◽  
Evelyne Gautier ◽  
Jean-Marie Jouannic ◽  
Pauline Ernault ◽  
...  
Keyword(s):  
Sex Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
First Trimester ◽  
Maternal Serum ◽  
Fetal Sex

scholarly journals Noninvasive Fetal Sex Determination by Real-Time PCR and TaqMan Probes

2020 ◽  
Vol 9 (3) ◽  
pp. 315-323
Author(s):  
Mohammad Hossein Ahmadi ◽  
Naser Amirizadeh ◽  
Maryam Rabiee ◽  
Fatemeh Rahimi-Sharbaf ◽  
Ali Akbar Pourfathollah ◽  
...  
Keyword(s):  
Sex Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Fetal Sex ◽  
Taqman Probes

Non-invasive fetal ABO genotyping in maternal plasma using real-time PCR

2015 ◽  
Vol 53 (12) ◽  
Author(s):  
Wenqian Song ◽  
Shihang Zhou ◽  
Linnan Shao ◽  
Ni Wang ◽  
Lingzi Pan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Maternal Plasma ◽  
Forensic Analysis ◽  
Igg Antibody ◽  
Non Invasive ◽  
Abo Incompatibility ◽  
Cell Free Fetal Dna ◽  
Methylation Sensitive Restriction Enzyme ◽  
Maternal Igg

AbstractFetal-maternal ABO incompatibility is a frequent cause of hemolytic disease of the fetus and newborn (HDFN). The routine serological testing of maternal IgG antibody level to predict HDFN shows low reliability. Non-invasive fetal ABO genotyping could provide a new avenue for predicting ABO-HDFN in early pregnancy. The aim of our study is to investigate the feasibility of fetal ABO genotyping in maternal plasma with real-time PCR.Plasma samples were collected from a total of 73 blood group O pregnant women between 12 and 25 weeks of gestation, and then DNA was extracted from the maternal plasma containing cell-free fetal DNA (cffDNA). TaqMan-based real-time PCR was performed after methylation-sensitive restriction enzyme to detect hypermethylatedThe fetalWe have developed a rapid and reliable protocol for fetal ABO genotyping in maternal plasma using real-time PCR. This protocol is suitable for routine prenatal diagnose of HDFN and forensic analysis.

Non-Invasive Fetal RHD Exon 7 and Exon 10 Genotyping Using Real-Time PCR Testing of Fetal DNA in Maternal Plasma

2005 ◽  
Vol 20 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Ilona Hromadnikova ◽  
Lenka Vechetova ◽  
Klara Vesela ◽  
Blanka Benesova ◽  
Jindrich Doucha ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Maternal Plasma ◽  
Non Invasive ◽  
Fetal Dna ◽  
Exon 10

An Effective Method for Detecting Y-chromosome Specific Sequences of Circulating Fetal DNA in Maternal Plasma During the First-trimester

2019 ◽  
Author(s):  
Najmeh Davoodian ◽  
Ali Kadivar ◽  
Heidar Heidari Khoie ◽  
Sima Hematian Khayat ◽  
Mahboobeh Heidari Nasirabadi
Keyword(s):  
Prenatal Diagnosis ◽  
Pregnant Women ◽  
Real Time ◽  
Y Chromosome ◽  
Maternal Plasma ◽  
Fetal Sex ◽  
Non Invasive ◽  
Fetal Dna ◽  
Fetal Gender ◽  
Cell Free Fetal Dna

Background and Aims: New advances in the use of cell-free fetal DNA (cffDNA) in maternal plasma of pregnant women has provided the possibility of applying cffDNA in prenatal diagnosis as a non-invasive method. One of the applications of prenatal diagnosis is fetal gender determination. Early prenatal determination of fetal sex is required for pregnant women at risk of X-linked and some endocrine diseases. The present study was carried out to perform an efficient polymerase chain reaction (PCR) method in order to improve sensitivity, specificity and accuracy of non-invasive fetal gender detection using fetal DNA in maternal plasma during 8th -12th weeks of pregnancy. Materials and Methods: Thirty-five pregnant women with 8 to 12 weeks of pregnancy were selected for prenatal fetal sex determination. Maternal peripheral blood was collected and cffDNA was extracted from 3-ml of maternal plasma. Two multi copy Y-chromosome-specific region (DYS and DAZ) and a single copy gene (SRY) were amplified by real-time quantitative PCR. Amplification was labeled as positive, negative, or inconclusive according to a stringent algorithm. Results: Using this method, the sensitivity and specificity of the real-time PCR assay was 100% and 93.8% for prenatal fetal sex detection, respectively. Conclusions: It is concluded that fetal sex can be determined with a high level of accuracy by our algorithm, after 8 weeks of gestation with cffDNA analysis.

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