Fetal sex determination in twin pregnancies using cell free fetal DNA analysis

2018 ◽  
Vol 38 (8) ◽  
pp. 578-584 ◽  
Author(s):  
Miguel Milan ◽  
Emilia Mateu ◽  
David Blesa ◽  
Monica Clemente-Ciscar ◽  
Carlos Simon
Keyword(s):  
Sex Determination ◽  
Dna Analysis ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna ◽  
Twin Pregnancies

Noninvasive fetal sex determination by analysis of cell-free fetal DNA in maternal capillary blood obtained by fingertip puncture

2018 ◽  
Vol 38 (8) ◽  
pp. 620-623
Author(s):  
Ticiane Henriques Santa Rita ◽  
Camila Santos Nobre ◽  
Rafael Henriques Jácomo ◽  
Lídia Freire Abdalla Nery ◽  
Gustavo Barcelos Barra
Keyword(s):  
Sex Determination ◽  
Capillary Blood ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

scholarly journals Fetal Sex Determination Using Cell-Free Fetal Dna (cffDNA) in Maternal Blood

2016 ◽  
Vol 5 (2) ◽  
pp. 89 ◽  
Author(s):  
I Nyoman Hariyasa Sanjaya ◽  
Tjok Gde Agung Suwardewa ◽  
I G Kamasan N. Arijana
Keyword(s):  
Sex Determination ◽  
Maternal Blood ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

scholarly journals Noninvasive Fetal Sex Determination Using Cell-Free Fetal DNA

JAMA ◽  
2011 ◽  
Vol 306 (6) ◽  
Author(s):  
Stephanie A. Devaney ◽  
Glenn E. Palomaki ◽  
Joan A. Scott ◽  
Diana W. Bianchi
Keyword(s):  
Sex Determination ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

scholarly journals Evaluation of Sample Stability and Automated DNA Extraction for Fetal Sex Determination Using Cell-Free Fetal DNA in Maternal Plasma

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Elena Ordoñez ◽  
Laura Rueda ◽  
M. Paz Cañadas ◽  
Carme Fuster ◽  
Vincenzo Cirigliano
Keyword(s):  
Sex Determination ◽  
Dna Extraction ◽  
Maternal Plasma ◽  
Daily Routine ◽  
Reference Laboratory ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Sample Stability ◽  
Cell Free Fetal Dna ◽  
Automated Dna Extraction

Objective. The detection of paternally inherited sequences in maternal plasma, such as the SRY gene for fetal sexing or RHD for fetal blood group genotyping, is becoming part of daily routine in diagnostic laboratories. Due to the low percentage of fetal DNA, it is crucial to ensure sample stability and the efficiency of DNA extraction. We evaluated blood stability at 4°C for at least 24 hours and automated DNA extraction, for fetal sex determination in maternal plasma.Methods. A total of 158 blood samples were collected, using EDTA-K tubes, from women in their 1st trimester of pregnancy. Samples were kept at 4°C for at least 24 hours before processing. An automated DNA extraction was evaluated, and its efficiency was compared with a standard manual procedure. The SRY marker was used to quantify cfDNA by real-time PCR.Results. Although lower cfDNA amounts were obtained by automated DNA extraction (mean 107,35 GE/mL versus 259,43 GE/mL), the SRY sequence was successfully detected in all 108 samples from pregnancies with male fetuses.Conclusion. We successfully evaluated the suitability of standard blood tubes for the collection of maternal blood and assessed samples to be suitable for analysis at least 24 hours later. This would allow shipping to a central reference laboratory almost from anywhere in Europe.

Fetal sex determination using circulating cell-free fetal DNA (ccffDNA) at 11 to 13 weeks of gestation

2010 ◽  
Vol 30 (10) ◽  
pp. 918-923 ◽  
Author(s):  
Ranjit Akolekar ◽  
Daniel H. Farkas ◽  
Anna L. VanAgtmael ◽  
Allan T. Bombard ◽  
Kypros H. Nicolaides
Keyword(s):  
Sex Determination ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

scholarly journals Cell-Free RNA Is a Reliable Fetoplacental Marker in Noninvasive Fetal Sex Determination

2015 ◽  
Vol 61 (12) ◽  
pp. 1515-1523 ◽  
Author(s):  
Elke Mersy ◽  
Brigitte H W Faas ◽  
Sabine Spierts ◽  
Leonie M H Houben ◽  
Merryn V E Macville ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Sex Determination ◽  
Prenatal Testing ◽  
Plasma Samples ◽  
Rt Pcr ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna ◽  
One Step ◽  
Reliable Marker

Abstract BACKGROUND Noninvasive genetic tests that use cell-free fetal DNA (cffDNA) are used increasingly in prenatal care. A low amount of cffDNA can have detrimental effects on the reliability of these tests. A marker to confirm the presence of fetal nucleic acids is therefore required that is universally applicable and easy to incorporate. METHODS We developed a novel multiplex, single-tube, noninvasive fetal sex determination assay by combining amplification of AMELY cffDNA with one-step reverse transcription (RT)-PCR of trophoblast-derived cell-free RNA (cfRNA), which functions as a sex-independent fetoplacental marker. We tested plasma samples from 75 pregnant women in duplicate in a blinded fashion. The fetus was considered to be male in the case of a positive result for AMELY and cfRNA amplification in both RT-PCRs. The fetus was considered to be female in the case of negative AMELY and positive cfRNA result in both RT-PCRs. In other cases, the test was repeated. We compared the results with invasive prenatal testing and pregnancy outcomes. RESULTS The AMELY cffDNA amplification and cfRNA result was unambiguous and identical in duplicate in 71 of 75 plasma samples (95%). Four samples (5%) required an extra replicate because of an absent fetoplacental marker. Thereafter, fetal sex was correctly determined in all 75 plasma samples. CONCLUSIONS Amplification of trophoblast-derived cfRNA is a reliable marker for the confirmation of the presence of fetoplacentally derived nucleic acids in noninvasive fetal sex determination.

scholarly journals Clinical application of fetal sex determination using cell-free fetal DNA in pregnant carriers of X-linked genetic disorders

2011 ◽  
Vol 56 (4) ◽  
pp. 296-299 ◽  
Author(s):  
Kiyonori Miura ◽  
Ai Higashijima ◽  
Takako Shimada ◽  
Shoko Miura ◽  
Kentaro Yamasaki ◽  
...  
Keyword(s):  
Sex Determination ◽  
Clinical Application ◽  
Genetic Disorders ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

Implementing noninvasive prenatal fetal sex determination using cell-free fetal DNA in the United Kingdom

2012 ◽  
Vol 12 (sup1) ◽  
pp. S119-S126 ◽  
Author(s):  
Melissa Hill ◽  
Celine Lewis ◽  
Lucy Jenkins ◽  
Stephanie Allen ◽  
Rob G Elles ◽  
...  
Keyword(s):  
United Kingdom ◽  
Sex Determination ◽  
Fetal Sex ◽  
Fetal Dna ◽  
The United Kingdom ◽  
Cell Free Fetal Dna
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