Validation of automatic scanning of microscope slides in recovering rare cellular events: application for detection of fetal cells in maternal blood

2014 ◽  
Vol 34 (6) ◽  
pp. 538-546 ◽  
Author(s):  
Ahmed Emad ◽  
Eric F. Bouchard ◽  
Josée Lamoureux ◽  
Annie Ouellet ◽  
Aparajita Dutta ◽  
...  
Keyword(s):  
Maternal Blood ◽  
Fetal Cells ◽  
Cellular Events ◽  
Microscope Slides ◽  
Automatic Scanning

scholarly journals Efficiency of Manual Scanning in Recovering Rare Cellular Events Identified by FluorescenceIn SituHybridization: Simulation of the Detection of Fetal Cells in Maternal Blood

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Ahmed Emad ◽  
Seemi Ayub ◽  
Oumar Samassékou ◽  
Marie-Chantal Grégoire ◽  
Macoura Gadji ◽  
...  
Keyword(s):  
Efficient Method ◽  
Unit Volume ◽  
Detection Efficiency ◽  
Maternal Blood ◽  
Fetal Cells ◽  
Cellular Events ◽  
Probe Hybridization ◽  
Rare Cells

Fluorescencein situhybridization (FISH) and manual scanning is a widely used strategy for retrieving rare cellular events such as fetal cells in maternal blood. In order to determine the efficiency of these techniques in detection of rare cells, slides of XX cells with predefined numbers (1–10) of XY cells were prepared. Following FISH hybridization, the slides were scanned blindly for the presence of XY cells by different observers. The average detection efficiency was 84% (125/148). Evaluation of probe hybridization in the missed events showed that 9% (2/23) were not hybridized, 17% (4/23) were poorly hybridized, while the hybridization was adequate for the remaining 74% (17/23). In conclusion, manual scanning is a relatively efficient method to recover rare cellular events, but about 16% of the events are missed; therefore, the number of fetal cells per unit volume of maternal blood has probably been underestimated when using manual scanning.

scholarly journals Development of a Specific Monoclonal Antibody to Detect Male Cells Expressing the RPS4Y1 Protein

2021 ◽  
Vol 22 (4) ◽  
pp. 2001
Author(s):  
Silvia Spena ◽  
Chiara Cordiglieri ◽  
Isabella Garagiola ◽  
Flora Peyvandi
Keyword(s):  
Monoclonal Antibody ◽  
Prenatal Diagnosis ◽  
Maternal Blood ◽  
Limiting Factors ◽  
Inherited Disease ◽  
Specific Monoclonal Antibody ◽  
Native Form ◽  
Fetal Cells ◽  
Non Invasive ◽  
Reliable Isolation

Hemophilia is an X-linked recessive bleeding disorder. In pregnant women carrier of hemophilia, the fetal sex can be determined by non-invasive analysis of fetal DNA circulating in the maternal blood. However, in case of a male fetus, conventional invasive procedures are required for the diagnosis of hemophilia. Fetal cells, circulating in the maternal bloodstream, are an ideal target for a safe non-invasive prenatal diagnosis. Nevertheless, the small number of cells and the lack of specific fetal markers have been the most limiting factors for their isolation. We aimed to develop monoclonal antibodies (mAbs) against the ribosomal protein RPS4Y1 expressed in male cells. By Western blotting, immunoprecipitation and immunofluorescence analyses performed on cell lysates from male human hepatoma (HepG2) and female human embryonic kidney (HEK293) we developed and characterized a specific monoclonal antibody against the native form of the male RPS4Y1 protein that can distinguish male from female cells. The availability of the RPS4Y1-targeting monoclonal antibody should facilitate the development of novel methods for the reliable isolation of male fetal cells from the maternal blood and their future use for non-invasive prenatal diagnosis of X-linked inherited disease such as hemophilia.

The use ofin vitro expanded erythroid cells in a model system for the isolation of fetal cells from maternal blood

1999 ◽  
Vol 19 (4) ◽  
pp. 323-329 ◽  
Author(s):  
Mieke W. J. C. Jansen ◽  
Marieke von Lindern ◽  
Hartmut Beug ◽  
Helen Brandenburg ◽  
Hajo I. J. Wildschut ◽  
...  
Keyword(s):  
Maternal Blood ◽  
Model System ◽  
Erythroid Cells ◽  
Fetal Cells

Fetal Cells in Maternal Blood

1963 ◽  
Vol 56 (7) ◽  
pp. 782-783 ◽  
Author(s):  
WILLIS E. BROWN ◽  
GORDON T. COWLES
Keyword(s):  
Maternal Blood ◽  
Fetal Cells

Choice of anticoagulant can influence the analysis using fluorescencein situ hybridization of fetal cells enriched from maternal blood

2000 ◽  
Vol 20 (3) ◽  
pp. 257-259 ◽  
Author(s):  
Verena Brombacher ◽  
Vivian Kiefer ◽  
Carolyn Troeger ◽  
Yvan Vial ◽  
Sabine Minderer ◽  
...  
Keyword(s):  
Maternal Blood ◽  
Fluorescencein Situ Hybridization ◽  
Fetal Cells

scholarly journals Enrichment, identification and analysis of fetal cells from maternal blood: evaluation of a prenatal diagnosis system

1999 ◽  
Vol 19 (7) ◽  
pp. 648-652 ◽  
Author(s):  
Irene M. de Graaf ◽  
Marja E. Jakobs ◽  
Nico J. Leschot ◽  
Ilya Ravkin ◽  
Simon Goldbard ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Maternal Blood ◽  
Diagnosis System ◽  
Fetal Cells
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