Comment on “MeDIP real-time qPCR of maternal peripheral blood reliably identifies trisomy 21”

2013 ◽  
Vol 33 (4) ◽  
pp. 403-403 ◽  
Author(s):  
Han Qin ◽  
Michael Bonifacio ◽  
Steven McArthur ◽  
Andrew McLennan ◽  
Tom Boogert ◽  
...  
Keyword(s):  
Real Time ◽  
Peripheral Blood ◽  
Trisomy 21 ◽  
Maternal Peripheral Blood ◽  
Real Time Qpcr

MeDIP real-time qPCR of maternal peripheral blood reliably identifies trisomy 21

2012 ◽  
Vol 32 (10) ◽  
pp. 996-1001 ◽  
Author(s):  
Evdokia Tsaliki ◽  
Elisavet A. Papageorgiou ◽  
Christiana Spyrou ◽  
George Koumbaris ◽  
Elena Kypri ◽  
...  
Keyword(s):  
Real Time ◽  
Peripheral Blood ◽  
Trisomy 21 ◽  
Maternal Peripheral Blood ◽  
Real Time Qpcr

scholarly journals Prenatal Diagnosis of Fetal Trisomy 21 from Maternal Peripheral Blood

2003 ◽  
Vol 44 (2) ◽  
pp. 181 ◽  
Author(s):  
Young Ho Yang ◽  
Sung Hoon Kim ◽  
Eun Suk Yang ◽  
Sei Kwang Kim ◽  
In Kyu Kim ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Peripheral Blood ◽  
Trisomy 21 ◽  
Maternal Peripheral Blood

scholarly journals A comparison of DNA methylation specific droplet digital PCR (ddPCR) and real time qPCR with flow cytometry in characterizing human T cells in peripheral blood

Epigenetics ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. 1360-1365 ◽  
Author(s):  
John K Wiencke ◽  
Paige M Bracci ◽  
George Hsuang ◽  
Shichun Zheng ◽  
Helen Hansen ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Flow Cytometry ◽  
T Cells ◽  
Real Time ◽  
Peripheral Blood ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Human T Cells ◽  
Real Time Qpcr

scholarly journals Probe-Based Real-Time qPCR Assays for a Reliable Differentiation of Capripox Virus Species

2021 ◽  
Vol 9 (4) ◽  
pp. 765
Author(s):  
Janika Wolff ◽  
Martin Beer ◽  
Bernd Hoffmann
Keyword(s):  
Real Time ◽  
Animal Health ◽  
Diagnostic Methods ◽  
Virus Species ◽  
Time Saving ◽  
Robust Detection ◽  
Highly Sensitive ◽  
Reliable Differentiation ◽  
World Organization ◽  
Real Time Qpcr

Outbreaks of the three capripox virus species, namely lumpy skin disease virus, sheeppox virus, and goatpox virus, severely affect animal health and both national and international economies. Therefore, the World Organization for Animal Health (OIE) classified them as notifiable diseases. Until now, discrimination of capripox virus species was possible by using different conventional PCR protocols. However, more sophisticated probe-based real-time qPCR systems addressing this issue are, to our knowledge, still missing. In the present study, we developed several duplex qPCR assays consisting of different types of fluorescence-labelled probes that are highly sensitive and show a high analytical specificity. Finally, our assays were combined with already published diagnostic methods to a diagnostic workflow that enables time-saving, reliable, and robust detection, differentiation, and characterization of capripox virus isolates.

Non-invasive approach to prenatal diagnosis from maternal peripheral blood

1992 ◽  
Vol 12 (6) ◽  
pp. 547-548 ◽  
Author(s):  
Y.-M. D. Lo ◽  
J. S. Wainscoat ◽  
K. A. Fleming
Keyword(s):  
Prenatal Diagnosis ◽  
Peripheral Blood ◽  
Invasive Approach ◽  
Non Invasive ◽  
Maternal Peripheral Blood
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