Isolation of serum nucleic acids for fetal DNA analysis: comparison of manual and automated extraction methods

2008 ◽  
Vol 28 (13) ◽  
pp. 1227-1231 ◽  
Author(s):  
Irina Banzola ◽  
Inès Kaufmann ◽  
Olav Lapaire ◽  
Sinuhe Hahn ◽  
Wolfgang Holzgreve ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dna Analysis ◽  
Extraction Methods ◽  
Automated Extraction ◽  
Fetal Dna

Method for automated extraction and purification of nucleic acids and its implementation in microfluidic system

2011 ◽  
Vol 47 (2) ◽  
pp. 211-220 ◽  
Author(s):  
D. D. Mamaev ◽  
D. A. Khodakov ◽  
E. I. Dementieva ◽  
I. V. Filatov ◽  
D. A. Yurasov ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Microfluidic System ◽  
Automated Extraction ◽  
Extraction And Purification

scholarly journals Current Perspectives on High-Throughput Sequencing (HTS) for Adventitious Virus Detection: Upstream Sample Processing and Library Preparation

Viruses ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 566 ◽  
Author(s):  
Siemon Ng ◽  
Cassandra Braxton ◽  
Marc Eloit ◽  
Szi Feng ◽  
Romain Fragnoud ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Nucleic Acids ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Virus Detection ◽  
Extraction Methods ◽  
Control Measures ◽  
Acid Extraction ◽  
Library Preparation ◽  
Sample Processing

A key step for broad viral detection using high-throughput sequencing (HTS) is optimizing the sample preparation strategy for extracting viral-specific nucleic acids since viral genomes are diverse: They can be single-stranded or double-stranded RNA or DNA, and can vary from a few thousand bases to over millions of bases, which might introduce biases during nucleic acid extraction. In addition, viral particles can be enveloped or non-enveloped with variable resistance to pre-treatment, which may influence their susceptibility to extraction procedures. Since the identity of the potential adventitious agents is unknown prior to their detection, efficient sample preparation should be unbiased toward all different viral types in order to maximize the probability of detecting any potential adventitious viruses using HTS. Furthermore, the quality assessment of each step for sample processing is also a critical but challenging aspect. This paper presents our current perspectives for optimizing upstream sample processing and library preparation as part of the discussion in the Advanced Virus Detection Technologies Interest group (AVDTIG). The topics include: Use of nuclease treatment to enrich for encapsidated nucleic acids, techniques for amplifying low amounts of virus nucleic acids, selection of different extraction methods, relevant controls, the use of spike recovery experiments, and quality control measures during library preparation.

scholarly journals Optimal non‐invasive diagnosis of fetal achondroplasia combining ultrasonography with circulating cell‐free fetal DNA analysis

2018 ◽  
Vol 53 (1) ◽  
pp. 87-94 ◽  
Author(s):  
A. J. Vivanti ◽  
J.‐M. Costa ◽  
A. Rosefort ◽  
P. Kleinfinger ◽  
L. Lohmann ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Non Invasive ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

Cell-free Fetal DNA Analysis in Maternal Plasma as Screening Test for Trisomies 21, 18, and 13 in Twin Pregnancy

2019 ◽  
Vol 74 (2) ◽  
pp. 61-63
Author(s):  
G. Le Conte ◽  
A. Letourneau ◽  
J. Jani ◽  
P. Kleinfinger ◽  
L. Lohmann ◽  
...  
Keyword(s):  
Screening Test ◽  
Twin Pregnancy ◽  
Dna Analysis ◽  
Maternal Plasma ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

scholarly journals IN VITRO BINDING OF NUCLEIC ACIDS TO TISSUE SECTIONS AFTER REMOVAL OF TISSUE NUCLEIC ACIDS

1964 ◽  
Vol 12 (8) ◽  
pp. 640-645 ◽  
Author(s):  
R. DAOUST
Keyword(s):  
Nucleic Acids ◽  
Extraction Methods ◽  
Organ Specificity ◽  
Chemical Extraction ◽  
Nucleic Acid Binding ◽  
Cytoplasmic Staining ◽  
Tissue Sections ◽  
In Vitro Binding ◽  
Vitro Binding

Rat tissue sections were freed from nucleic acids by enzymatic or chemical extraction methods, immersed in solutions of nucleic acids from various sources and stained with toluidine blue. Tissue sections immersed in solutions of DNA showed intense nuclear and cytoplasmic staining; similar results were obtained with tissue sections placed in solutions of RNA. Thus both DNA and RNA can bind to nuclear and cytoplasmic sites in tissue sections freed from nucleic acids. The experiments indicated however that in vitro binding of nucleic acids to tissue sections was not specific to original sites of nucleic acid binding and the reactions showed no particular species or organ specificity.

Automated and semi-automated extraction methods for GC–MS determination of pesticides in environmental samples

2016 ◽  
Vol 12 ◽  
pp. 1-12 ◽  
Author(s):  
Irene Domínguez ◽  
Roberto Romero González ◽  
F. Javier Arrebola Liébanas ◽  
José L. Martínez Vidal ◽  
Antonia Garrido Frenich
Keyword(s):  
Environmental Samples ◽  
Extraction Methods ◽  
Automated Extraction

scholarly journals Sticky problems: extraction of nucleic acids from molluscs

2021 ◽  
Vol 376 (1825) ◽  
Author(s):  
Coen M. Adema
Keyword(s):  
Nucleic Acids ◽  
Dna Extraction ◽  
Extraction Methods ◽  
Biological Properties ◽  
Alternative Methods ◽  
28S Rrna ◽  
Future Directions ◽  
Dna And Rna ◽  
Standard Quality ◽  
Dna Extraction Methods

Traditional molecular methods and omics-techniques across molluscan taxonomy increasingly inform biology of Mollusca. Recovery of DNA and RNA for such studies is challenged by common biological properties of the highly diverse molluscs. Molluscan biomineralization, adhesive structures and mucus involve polyphenolic proteins and mucopolysaccharides that hinder DNA extraction or copurify to inhibit enzyme-catalysed molecular procedures. DNA extraction methods that employ the detergent hexadecyltrimethylammoniumbromide (CTAB) to remove these contaminants importantly facilitate molecular-level study of molluscs. Molluscan pigments may stain DNA samples and interfere with spectrophotometry, necessitating gel electrophoresis or fluorometry for accurate quantification. RNA can reliably be extracted but the ‘hidden break’ in 28S rRNA of molluscs (like most protostomes) causes 18S and 28S rRNA fragments to co-migrate electrophoretically. This challenges the standard quality control based on the ratio of 18S and 28S rRNA, developed for deuterostome animals. High-AT content in molluscan rRNA prevents the effective purification of polyadenylated mRNA. Awareness of these matters aids the continuous expansion of molecular malacology, enabling work also with museum specimens and next-generation sequencing, with the latter imposing unprecedented demands on DNA quality. Alternative methods to extract nucleic acids from molluscs are available from literature and, importantly, from communications with others who study the molecular biology of molluscs. This article is part of the Theo Murphy meeting issue ‘Molluscan genomics: broad insights and future directions for a neglected phylum’.

scholarly journals Automated Assay for Fetal DNA Analysis in Maternal Serum

2002 ◽  
Vol 48 (4) ◽  
pp. 679-680 ◽  
Author(s):  
Jean-Marc Costa ◽  
Pauline Ernault
Keyword(s):  
Dna Analysis ◽  
Maternal Serum ◽  
Fetal Dna ◽  
Automated Assay
Sign in / Sign up

Export Citation Format

Share Document