Problems in prenatal diagnosis of β-thalassaemia by fetal blood analysis: β-chain variant comigrating with gamma chains

1988 ◽  
Vol 8 (5) ◽  
pp. 393-397
Author(s):  
C. Rosatelli ◽  
F. Schettini ◽  
G. Monni ◽  
T. Tuveri ◽  
M. T. Scalas ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Blood Analysis ◽  
Fetal Blood ◽  
Β Chain ◽  
Chain Variant

Usefulness of automated chromatography for rapid fetal blood analysis for second trimester prenatal diagnosis of ?-thalassemia

2002 ◽  
Vol 22 (2) ◽  
pp. 153-157 ◽  
Author(s):  
Mahrukh R. Wadia ◽  
Supriya P. Phanasgaokar ◽  
Anita H. Nadkarni ◽  
Reema R. Surve ◽  
Ajit C. Gorakshakar ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Blood Analysis ◽  
Second Trimester ◽  
Fetal Blood

Prenatal diagnosis of thalassemia major by fetal blood analysis: Experience with 1000 cases

1986 ◽  
Vol 6 (3) ◽  
pp. 159-167 ◽  
Author(s):  
Antonio Cao ◽  
Angela M. Falchi ◽  
Teresa Tuveri ◽  
Maria T. Scalas ◽  
Giovanni Monni ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Thalassemia Major ◽  
Blood Analysis ◽  
Fetal Blood

Synthesis of hemoglobin Cranston, an elongated β chain variant

1980 ◽  
Vol 140 (3) ◽  
pp. 377-389 ◽  
Author(s):  
Joseph R. Shaeffer ◽  
Geoffrey J. Schmidt ◽  
Robert E. Kingston ◽  
H.Franklin Bunn
Keyword(s):  
Β Chain ◽  
Chain Variant

Hb Hinwil or β38(C4)THR→ASN: A new β Chain Variant Detected in a Swiss Family

Hemoglobin ◽  
1996 ◽  
Vol 20 (1) ◽  
pp. 31-40 ◽  
Author(s):  
H. Frischknecht ◽  
M. Ventruto ◽  
D. Hess ◽  
P. Hunziker ◽  
M. C. Rosatelli ◽  
...  
Keyword(s):  
Β Chain ◽  
Chain Variant

scholarly journals Concentration of Fetal Red Blood Cells From a Mixture of Maternal and Fetal Blood by Anti-i Serum—An Aid to Prenatal Diagnosis of Hemoglobinopathies

Blood ◽  
1974 ◽  
Vol 43 (3) ◽  
pp. 411-415 ◽  
Author(s):  
Yuet Wai Kan ◽  
David G. Nathan ◽  
Gabriel Cividalli ◽  
Marie C. Crookston
Keyword(s):  
Prenatal Diagnosis ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Red Cells ◽  
Fetal Blood ◽  
Fetal Cells

Abstract Fetal red blood cells were concentrated from mixtures of maternal and fetal cells by differential agglutination with anti-i serum. This method will be useful for prenatal diagnosis of hemoglobinopathies when blood obtained from the fetus is heavily contaminated by maternal cells. The method is practical, except in very rare cases in which the maternal red cells are strongly agglutinated by anti-i.

Non-invasive prenatal diagnosis for fetal blood group status

2014 ◽  
pp. 173-182
Author(s):  
Geoff Daniels ◽  
Kirstin Finning ◽  
Peter Martin ◽  
Edwin Massey
Keyword(s):  
Prenatal Diagnosis ◽  
Blood Group ◽  
Fetal Blood ◽  
Group Status ◽  
Non Invasive

Immunological Cross Reaction Between Chains of Human Fibrinogen

1975 ◽  
Author(s):  
B. Oårdlund
Keyword(s):  
Electrophoretic Mobility ◽  
Thick Layer ◽  
Strong Reaction ◽  
Human Fibrinogen ◽  
Crossed Immunoelectrophoresis ◽  
Antigenic Sites ◽  
Barbital Buffer ◽  
Β Chain ◽  
Immunological Cross Reaction ◽  
Chain Variant

Employing the crossed immunoelectrophoresis technique (CIE) the antigenic composition of fibrinogen chains and heterogeneity in their electrophoretic mobility was investigated.Highly purified chains of fibrinogen were prepared by chromatography on CM-cellulose (1). Antisera against chains were prepared by immunizing rabbits with Aα, Bβ and γ chains respectively. The CIE was performed on 10 × 10 glass plates with an 1 mm thick layer of 1% agarose in 0.04 M barbital buffer pH 8.6. The electrophoresis was run for 1 h with 12 V/cm. Four fifth of the gel was cut off and replaced with the same agarose solution containing antibodies. The second dimension electrophoresis was run for about 20 h with 2 V/cm.Using anti Bβ chain serum a partial immunological identity was revealed between the Bβ chain and the γ chain. The Bβ chain has except for the antigenic sites in common with the γ chain additional sites that are unique for the B β chain. The results were confirmed with the anti γ chain serum which gave a strong reaction with both the γ chain and the B β chain preparations. The Aα chain did not react with γ antiserum, nor did the γ chain react with anti Aα chain serum. The immunological relation between the Aα chain and the B β chain is under investigation.These experiments also concluded that the γ chain preparation is contaminated with a B β chain variant that has an electrophoretic mobility similar to that of the γ chain. Also the Bβ chain seemed to be impure due to contamination by an Aα chain derivative with higher electrophoretic mobility.(1) Murano, G., Wiman, B., Blomböck, B. and Blomböck, M. : Preparation and isolation of the S-carboxymethyl derivative chains of human fibrinogen. F.E.B.S. Letters: 14, 37, 1971.

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