Rapid prenatal diagnosis of common trisomies: discordant results between QF-PCR analysis and karyotype analysis on long-term culture for a case of trisomy 18 detected in CVS

2006 ◽  
Vol 26 (12) ◽  
pp. 1160-1167 ◽  
Author(s):  
S. K. Allen ◽  
A. Luharia ◽  
C. P. Gould ◽  
F. MacDonald ◽  
S. Larkins ◽  
...  
Keyword(s):  
Prenatal Diagnosis ◽  
Karyotype Analysis ◽  
Trisomy 18 ◽  
Pcr Analysis ◽  
Long Term Culture

scholarly journals qPCR analysis of mesenchymal stem cell marker expression during the long-term culture of canine adipocyte derived stem cells

2020 ◽  
Vol 8 (4) ◽  
pp. 139-145
Author(s):  
Rut Bryl ◽  
Claudia Dompe ◽  
Maurycy Jankowski ◽  
Katarzyna Stefańska ◽  
Afsaneh Golkar Narenji ◽  
...  
Keyword(s):  
Stem Cells ◽  
Molecular Mechanisms ◽  
Cultured Cells ◽  
Stem Cell Marker ◽  
Pcr Analysis ◽  
Marker Genes ◽  
Long Term Culture ◽  
Marker Expression

AbstractDue to its availability and accessibility, adipose tissue has been the subject of various studies in many different medical fields and is believed to be a useful source of stem cells. The ability of ASCs to differentiate towards different cell lineages, with possibility of directing this differentiation, increases their possible clinical applications, and they have been widely employed in multiple therapies and treatment of different pathologies. However, a deeper understanding of the molecular mechanisms underlying the ASCs osteoblastic and chondrocyte differentiation may lead to novel applications treating a multitude of different bone-related diseases through techniques more likely meeting worldwide consensus. In this study, the RT-qPCR method was used to determine the changes in expression of ASC specific markers (CD105, CD73, CD14, CD34, CD90 and CD45) before and after long-term (14-day) in vitro cultures. To confirm the identity of the investigated cells, flow cytometry was used to evaluate the presence of positive (CD44, CD90) and negative (CD45, CD34) ASC markers. Overall, the results of the PCR analysis showed a significant change in expression of most of the marker genes, indicating significant changes in the cultured cells caused by their long-term culture, potentially altering their original stem-like characteristics.Running title: ASC marker expression during long-term in vitro culture

Rapid prenatal diagnosis by fluorescent in situ hybridization of chorionic villi: An adjunct to long-term culture and karyotype

1992 ◽  
Vol 167 (6) ◽  
pp. 1522-1525 ◽  
Author(s):  
Mark I. Evans ◽  
Katherine W. Klinger ◽  
Nelson B. Isada ◽  
Donna Shook ◽  
Wolfgang Holzgreve ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Prenatal Diagnosis ◽  
Fluorescent In Situ Hybridization ◽  
Chorionic Villi ◽  
Long Term Culture

scholarly journals Provision of exogenous lipid interacts with maternal BMI to affect human embryo survival in long term culture

2017 ◽  
Vol 108 (3) ◽  
pp. e59-e60
Author(s):  
R. Kile ◽  
Y. Yuan ◽  
H. Silz ◽  
S. McCormick ◽  
W.B. Schoolcraft ◽  
...  
Keyword(s):  
Human Embryo ◽  
Embryo Survival ◽  
Long Term Culture ◽  
Exogenous Lipid ◽  
Maternal Bmi

scholarly journals Long-term culture of SH-SY5Y neuroblastoma cells in the absence of neurotrophins: a novel model of neuronal ageing

2021 ◽  
pp. 109301
Author(s):  
Lisa Strother ◽  
Gareth B. Miles ◽  
Alison R. Holiday ◽  
Ying Cheng ◽  
Gayle H. Doherty
Keyword(s):  
Neuroblastoma Cells ◽  
Long Term Culture ◽  
Novel Model

Alteration and preservation of cellular characteristics in long-term culture of tetraploid H-1 (ES) cells

Human Cell ◽  
2008 ◽  
Vol 21 (2) ◽  
pp. 18-27 ◽  
Author(s):  
Kohzaburo FUJIKAWA-YAMAMOTO ◽  
Minoru MIYAGOSHI ◽  
Hiroko YAMAGISHI ◽  
Xianwen LUO
Keyword(s):  
Es Cells ◽  
Long Term Culture
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