Forty-eight-hour first-trimester glucose profiles in women with type 1 diabetes mellitus: a report of three cases of congenital malformation

2006 ◽  
Vol 26 (2) ◽  
pp. 123-127 ◽  
Author(s):  
Anneloes Kerssen ◽  
Harold W. de Valk ◽  
Gerard H. A. Visser
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Congenital Malformation ◽  
First Trimester ◽  
Glucose Profiles

scholarly journals Type 1 Diabetes Mellitus and the First Trimester Placenta: Hyperglycemia-Induced Effects on Trophoblast Proliferation, Cell Cycle Regulators, and Invasion

2021 ◽  
Vol 22 (20) ◽  
pp. 10989
Author(s):  
Alejandro Majali-Martinez ◽  
Ursula Weiss-Fuchs ◽  
Heidi Miedl ◽  
Desiree Forstner ◽  
Julia Bandres-Meriz ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Cell Cycle ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
First Trimester ◽  
Trophoblast Invasion ◽  
Cell Cycle Distribution ◽  
Cell Cycle Regulators ◽  
Gestational Week

Type 1 diabetes mellitus (T1DM) is associated with reduced fetal growth in early pregnancy, but a contributing role of the placenta has remained elusive. Thus, we investigated whether T1DM alters placental development in the first trimester. Using a protein array, the level of 60 cell-cycle-related proteins was determined in human first trimester placental tissue (gestational week 5–11) from control (n = 11) and T1DM pregnancies (n = 12). Primary trophoblasts (gestational week 7–12, n = 32) were incubated in the absence (control) or presence of hyperglycemia (25 mM D-glucose) and hyperosmolarity (5.5 mM D-glucose + 19.5 mM D-mannitol). We quantified the number of viable and dead trophoblasts (CASY Counter) and assessed cell cycle distribution (FACS) and trophoblast invasion using a transwell assay. T1DM was associated with a significant (p < 0.05) downregulation of Ki67 (−26%), chk1 (−25%), and p73 (−26%). The number of viable trophoblasts was reduced under hyperglycemia (−23%) and hyperosmolarity (−18%), whereas trophoblast invasion was increased only under hyperglycemia (+6%). Trophoblast cell death and cell cycle distribution remained unaffected. Collectively, our data demonstrate that hyperglycemia decreases trophoblast proliferation as a potential contributing factor to the reduced placental growth in T1DM in vivo.

917-P: Features of Glucose Profiles in Newly Diagnosed Fulminant Type 1 Diabetes Mellitus

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 917-P
Author(s):  
LU GAN ◽  
BEISI LIN ◽  
YONGWEN ZHOU ◽  
JINHUA YAN ◽  
WEN XU ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Newly Diagnosed ◽  
Fulminant Type 1 Diabetes ◽  
Glucose Profiles ◽  
Fulminant Type
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