Cytogenetic analysis of trophoblasts by comparative genomic hybridization in embryo-fetal development anomalies

2001 ◽  
Vol 21 (8) ◽  
pp. 613-618 ◽  
Author(s):  
A. C. Tabet ◽  
A. Aboura ◽  
M. C. Dauge ◽  
F. Audibert ◽  
A. Coulomb ◽  
...  
Keyword(s):  
Comparative Genomic Hybridization ◽  
Fetal Development ◽  
Cytogenetic Analysis ◽  
Comparative Genomic ◽  
Genomic Hybridization

Cytogenetic analysis from DNA by comparative genomic hybridization

2000 ◽  
Vol 43 (3-4) ◽  
pp. 147-154 ◽  
Author(s):  
Gérard Tachdjian ◽  
Azzedine Aboura ◽  
Jean Michel Lapierre ◽  
Franck Viguié
Keyword(s):  
Comparative Genomic Hybridization ◽  
Cytogenetic Analysis ◽  
Comparative Genomic ◽  
Genomic Hybridization

scholarly journals Cytogenetic analysis of spontaneously discharged products of conception by array-based comparative genomic hybridization

SpringerPlus ◽  
2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Nobuaki Ozawa ◽  
Haruhiko Sago ◽  
Kentaro Matsuoka ◽  
Tetsuo Maruyama ◽  
Ohsuke Migita ◽  
...  
Keyword(s):  
Comparative Genomic Hybridization ◽  
Cytogenetic Analysis ◽  
Comparative Genomic ◽  
Genomic Hybridization ◽  
Products Of Conception

scholarly journals Improved Sensitivity in Comparative Genomic Hybridization Analysis of DNA Heteroploid Cell Mixtures after Pre-Enrichment of Subpopulations by Fluorescence Activated Cell Sorting

1999 ◽  
Vol 19 (3-4) ◽  
pp. 119-124 ◽  
Author(s):  
Jacob Larsen ◽  
Maria Kirchhoff ◽  
Hanne Rose ◽  
Tommy Gerdes ◽  
Jan Maahr ◽  
...  
Keyword(s):  
Comparative Genomic Hybridization ◽  
Cell Sorting ◽  
Cytogenetic Analysis ◽  
Dilution Effect ◽  
Comparative Genomic ◽  
Fluorescence Activated Cell Sorting ◽  
Genomic Hybridization ◽  
Flow Sorting ◽  
K 562 Cells ◽  
The Individual

Cytogenetic analysis of solid tumors with comparative genomic hybridization (CGH) is hampered by the dilution of DNA from individual tumor subpopulations with DNA from other cells. We investigated to what extent this dilution effect can be alleviated using fluorescence activated cell sorting (flow sorting) of experimental DNA heteroploid cell mixtures prior to CGH. From mixtures of normal lymphocytes with triploid K‐562 cells the individual components were sorted according to stemline DNA content and processed by CGH in comparison with pure K‐562 samples and the original mixtures. Compared with 30 autosome copy number imbalances found in pure K‐562 samples, a mixture with 32% K‐562 cells showed 16 imbalancies, and none were detected in mixtures with 13% or 5% K‐562 cells. In contrast, 29, 22 and 23 imbalances were detected in K‐562 nuclei sorted from the 32%, 13% and 5% mixtures, respectively. This indicate that CGH analysis of flow sorted DNA aneuploid subpopulations enables a specific cytogenetic analysis of the individual subclones in a DNA heteroploid cell population.

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