Screening for oestrogenic activity of plant and food extracts usingin vitro trout hepatocyte cultures

2004 ◽  
Vol 15 (1) ◽  
pp. 40-45 ◽  
Author(s):  
C. Bennetau-Pelissero ◽  
K. Gontier Latonnelle ◽  
V. Lamothe ◽  
S. Shinkaruk-Poix ◽  
S. J. Kaushik
Keyword(s):  
Hepatocyte Cultures ◽  
Trout Hepatocyte

Two complementary bioassays for screening the estrogenic potency of xenobiotics: recombinant yeast for trout estrogen receptor and trout hepatocyte cultures

1997 ◽  
Vol 19 (3) ◽  
pp. 321-335 ◽  
Author(s):  
F Petit ◽  
P Le Goff ◽  
JP Cravedi ◽  
Y Valotaire ◽  
F Pakdel
Keyword(s):  
Estrogen Receptor ◽  
Polychlorinated Biphenyl ◽  
Estrogenic Activity ◽  
Wide Spectrum ◽  
Direct Interaction ◽  
Recombinant Yeast ◽  
Estrogenic Compounds ◽  
Hepatocyte Cultures ◽  
Trout Hepatocyte

A relation between the chemical structure of a xenobiotic and its steroidal action has not yet been clearly established. Thus, it is not possible to define the estrogenic potency of different xenobiotics. An assessment may be accomplished by the use of different bioassays. We have previously developed a yeast system highly and stably expressing rainbow trout estrogen receptor (rtER) in order to analyze the biological activity of the receptor. The recombinant yeast system appears to be a reliable, rapid and sensitive bioassay for the screening and determination of the direct interaction between ER and estrogenic compounds. This system was used in parallel with a more elaborate biological system, trout hepatocyte aggregate cultures, to examine the estrogenic potency of a wide spectrum of chemicals commonly found in the environment. In hepatocyte cultures, the vitellogenin gene whose expression is principally dependent upon estradiol was used as a biomarker. Moreover, competitive binding assays were performed to determine direct interaction between rtER and xenobiotics. In our study, 50% of the 49 chemical compounds tested exhibited estrogenic activity in the two bioassays: the herbicide diclofop-methyl; the fungicides biphenyl, dodemorph, and triadimefon; the insecticides lindane, methyl parathion, chlordecone, dieldrin, and endosulfan; polychlorinated biphenyl mixtures; the plasticizers or detergents alkylphenols and phthalates; and phytoestrogens. To investigate further biphenyl estrogenic activity, its principal metabolites were also tested in both bioassays. Among these estrogenic compounds, 70% were able to activate rtER in yeast and hepatocytes with variable induction levels according to the system. Nevertheless, 30% of these estrogenic compounds exhibited estrogenic activity in only one of the bioassays, suggesting the implication of metabolites or different pathways in the activation of gene transcription. This paper shows that it is important to combine in vivo bioassays with in vitro approaches to elucidate the mechanism of xenoestrogen actions.

Oxygen availability regulates metabolism and gene expression in trout hepatocyte cultures

2006 ◽  
Vol 291 (5) ◽  
pp. R1507-R1515 ◽  
Author(s):  
Eeva Rissanen ◽  
Hanna K. Tranberg ◽  
Mikko Nikinmaa
Keyword(s):  
Energy Metabolism ◽  
Metabolic Rate ◽  
Metabolic Regulation ◽  
Time Course ◽  
Oxygen Supply ◽  
Hypoxia Inducible Factor ◽  
Atp Content ◽  
Low Oxygen ◽  
Hepatocyte Cultures ◽  
Trout Hepatocyte

We studied the metabolic rate, cellular energetic state, hypoxia-inducible factor-1 (HIF-1) activation, and expression of enzymes involved in energy metabolism using rainbow trout ( Oncorhynchus mykiss) hepatocytes over the oxygen range from 21 to 1 kPa. Oxygen dependence of these factors was assessed by gradually reducing oxygen supply to cells from 21 kPa to 10, 5, 2, and 1 kPa. Moreover, time course experiments for up to 20 h at oxygen tensions of 1 and 2 kPa were carried out. Reduction of oxygen from 21 kPa to 10, 5, 2, and 1 kPa decreased metabolic rate of the cells by 14, 24, 37, and 46%, respectively. This response was instantaneous and fully reversible upon reoxygenation. Cellular ATP content and the expression of all mRNAs studied decreased when oxygen was reduced from 21 to 5 and 2 kPa. The lowest ATP levels, ∼43% of the initial value, were measured at 5 kPa of oxygen, whereas the reduction in mRNA amounts was most pronounced at 2 kPa. At 1 kPa oxygen tension, both ATP content and mRNA amounts returned to normoxic (21 kPa) levels with a concomitant activation of HIF-1, indicating reorganization of energy metabolism in adaptation of cells to low oxygen supply. These results show that oxygen has a direct regulatory effect on metabolism of trout hepatocyte cultures, supporting the view that oxygen has a profound role in metabolic regulation in cells.

Toxicological properties of thymoquinone in primary rat hepatocyte cultures

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
M Khader ◽  
N Bresgen ◽  
P Eckl
Keyword(s):  
Rat Hepatocyte ◽  
Hepatocyte Cultures

scholarly journals THE EXTRACELLULAR MATRIX CONTROL OF ACUTE PHASE RESPONSE IN RAT HEPATOCYTE CULTURES

Shock ◽  
1995 ◽  
Vol 4 (Supplement) ◽  
pp. 52
Author(s):  
E. Adoeva ◽  
T. Stepanova ◽  
I. Diakonov ◽  
A. Polevschlkov
Keyword(s):  
Extracellular Matrix ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Phase Response ◽  
Rat Hepatocyte ◽  
Hepatocyte Cultures ◽  
Matrix Control

scholarly journals Determinants of human apolipoprotein [a] secretion from mouse hepatocyte cultures

2001 ◽  
Vol 42 (1) ◽  
pp. 60-69 ◽  
Author(s):  
Jin Wang ◽  
Jennifer Boedeker ◽  
Helen H. Hobbs ◽  
Ann L. White
Keyword(s):  
Mouse Hepatocyte ◽  
Human Apolipoprotein ◽  
Hepatocyte Cultures ◽  
Apolipoprotein A
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