Comparative LC–MS analysis of tropolone alkaloids from in vitro cultures and native sources of Gloriosa superba by Kendrick mass defect plots

2020 ◽  
Author(s):  
Yancho Zarev ◽  
Pavlinka Popova ◽  
Kenn Foubert ◽  
Iliana Ionkova ◽  
Luc Pieters
Keyword(s):  
In Vitro Cultures ◽  
Mass Defect ◽  
Gloriosa Superba ◽  
Ms Analysis ◽  
Kendrick Mass Defect

LC-MS analysis of alkaloids in bulbs and in vitro cultures of Leucojum aestivum (Amaryllidaceae)

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
A Ptak ◽  
AE Tahchy ◽  
F Dupire ◽  
M Boisbrun ◽  
M Henry ◽  
...  
Keyword(s):  
In Vitro Cultures ◽  
Leucojum Aestivum ◽  
Ms Analysis

scholarly journals Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Indole Alkaloids Isolated from Catharanthus roseus (L.) G. Don Cultivated Conventionally and Derived from In vitro Cultures

2016 ◽  
Vol 44 (1) ◽  
pp. 100-106 ◽  
Author(s):  
Aneta WESOŁOWSKA ◽  
Monika GRZESZCZUK ◽  
Janusz WILAS ◽  
Danuta KULPA
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Catharanthus Roseus ◽  
Indole Alkaloids ◽  
In Vitro Cultures ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Temperature Method

Catharanthus roseus (periwinkle) is a medicinal plant commonly known for its wide biological activity. In many countries different parts of this plant are used for the treatment of diabetes, hypertension and for menstrual regulation. Due to the ability of production of alkaloids, which can be applied in cancer therapy, is still extensively investigated. Two, the mostvaluable alkaloids (vincristine and vinblastine) are present in C. roseus in very low concentrations. Micropropagation is promising technique used to enhance the level of important secondary metabolites. The main objective of present study was alkaloids extraction from plants cultivated conventionally and derived from in vitro cultures. In this order the aerial parts of periwinkle were extracted with 96% ethanol at room temperature (method I) and heated with 96% ethanol at 55 °C for 90 minutes (method II). The obtained mixtures of different indole alkaloids were analyzed by gas chromatography – mass spectrometry (GC-MS). Analysis revealed the presence of 15 alkaloids, among which vindoline,vindorosine,isovindolinineand ajmalicine were the most abundant. The obtained results indicated that the propagation method had a significant effect on the percentage content of alkaloids in C. roseus herb. Plants derived from in vitro cultures were richer in vindorosine and vindoline, while conventionally cultivated – in tetrahydroalstonine and ajmalicine. Moreover, in case of isovindolinine,vindolinine and ajmalicine, extraction at 55 °C was more effective, while for pericyclivine – maceration at room temperature.Interestingly, the pericyclivine was not detected in the mixture of alkaloids obtained from periwinkle herb by the extraction at55 °C.

Lignans from in vitro cultures of transgenic roots of Taxus x media var. Hicksii

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
K Sykłowska-Baranek ◽  
A Pietrosiuk ◽  
M Grech-Baran ◽  
M Bonfill ◽  
P Mistrzak
Keyword(s):  
In Vitro Cultures ◽  
Transgenic Roots ◽  
Taxus X Media

Phenolic compounds from in vitro cultures of Rindera graeca Boiss. & Feldr.

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
K Sykłowska-Baranek ◽  
A Pietrosiuk ◽  
K Graikou ◽  
H Damianakos ◽  
M Jeziorek ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
In Vitro Cultures

HPTLC determination of catechins in in-vitro cultures of two species of the genusPhyllanthus

2008 ◽  
Vol 21 (2) ◽  
pp. 103-106 ◽  
Author(s):  
Barbara Sparzak ◽  
Mirosława Krauze-Baranowska ◽  
Loretta Pobłocka-Olech
Keyword(s):  
In Vitro Cultures

GC-MS analysis and in vitro antibacterial potential of volatile chemical constituents from leaves of Murrayakoenigii L. Spreng

2017 ◽  
Vol VI (I) ◽  
pp. 143-147
Author(s):  
Itzaz Aslam ◽  
Faiza Aslam ◽  
Shahzad Qamar ◽  
Saiqa Ishtiaq ◽  
Muhammad Shaharyar Khan Afridi ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Ms Analysis ◽  
Antibacterial Potential
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