Untargeted metabolite profiling and phytochemical analysis based on RP‐HPLC‐DAD‐QTOF‐MS and MS/MS for discovering new bioactive compounds in Rumex algeriensis flowers and stems

2020 ◽  
Vol 31 (5) ◽  
pp. 616-635
Author(s):  
Sonda Ammar ◽  
Jouda Abidi ◽  
Simon Vlad Luca ◽  
Mahieddine Boumendjel ◽  
Krystyna Skalicka‐Woźniak ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Metabolite Profiling ◽  
Phytochemical Analysis ◽  
Rp Hplc ◽  
Untargeted Metabolite Profiling

Untargeted metabolite profiling and phytochemical analysis of Micromeria fruticosa L. (Lamiaceae) leaves

2019 ◽  
Vol 279 ◽  
pp. 128-143 ◽  
Author(s):  
Ibrahim M. Abu-Reidah ◽  
David Arráez-Román ◽  
Mohammed Al-Nuri ◽  
Ismail Warad ◽  
Antonio Segura-Carretero
Keyword(s):  
Metabolite Profiling ◽  
Phytochemical Analysis ◽  
Untargeted Metabolite Profiling ◽  
Micromeria Fruticosa

scholarly journals Untargeted Metabolite Profiling for Screening Bioactive Compounds in Digestate of Manure under Anaerobic Digestion

Water ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 2420 ◽  
Author(s):  
Jiaxin Lu ◽  
Atif Muhmood ◽  
Wojciech Czekała ◽  
Jakub Mazurkiewicz ◽  
Jacek Dach ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Bioactive Compounds ◽  
Metabolic Pathways ◽  
Metabolite Profiling ◽  
Swine Manure ◽  
Chicken Manure ◽  
Chemical Characteristics ◽  
Chemical Profiling ◽  
Different Types ◽  
Untargeted Metabolite Profiling

Untargeted metabolite profiling was performed on chicken manure (CHM), swine manure (SM), cattle manure (CM), and their respective digestate by XCMS coupled with MetaboAnalyst programs. Through global chemical profiling, the chemical characteristics of different digestates and types of manure were displayed during the anaerobic digestion (AD) process. As the feed for AD, CM had less easily-degradable organics, SM contained the least O-alkyls and anomerics of carbohydrates, and CHM exhibited relatively lower bio-stability. The derived metabolite pathways of different manure during the AD process were identified by MetaboAnalyst. Twelve, 8, and 5 metabolic pathways were affected by the AD process in CHM, SM, and CM, respectively. Furthermore, bioactive compounds of digestate were detected, such as amino acids (L-arginine, L-ornithine, L-cysteine, and L-aspartate), hormones (L-adrenaline, 19-hydroxy androstenedione, and estrone), alkaloids (tryptamine and N-methyltyramine), and vitamin B5, in different types of manure and their digestates. The combination of XCMS and MetaboAnalyst programs can be an effective strategy for metabolite profiling of manure and its anaerobic digestate under different situations.

scholarly journals MITO-Tag Mice enable rapid isolation and multimodal profiling of mitochondria from specific cell types in vivo

2018 ◽  
Vol 116 (1) ◽  
pp. 303-312 ◽  
Author(s):  
Erol C. Bayraktar ◽  
Lou Baudrier ◽  
Ceren Özerdem ◽  
Caroline A. Lewis ◽  
Sze Ham Chan ◽  
...  
Keyword(s):  
Metabolite Profiling ◽  
Cultured Cells ◽  
Transgenic Animals ◽  
Cell Types ◽  
Tissue Level ◽  
Specific Cell ◽  
Mammalian Tissues ◽  
Cell Type Specific ◽  
Untargeted Metabolite Profiling

Mitochondria are metabolic organelles that are essential for mammalian life, but the dynamics of mitochondrial metabolism within mammalian tissues in vivo remains incompletely understood. While whole-tissue metabolite profiling has been useful for studying metabolism in vivo, such an approach lacks resolution at the cellular and subcellular level. In vivo methods for interrogating organellar metabolites in specific cell types within mammalian tissues have been limited. To address this, we built on prior work in which we exploited a mitochondrially localized 3XHA epitope tag (MITO-Tag) for the fast isolation of mitochondria from cultured cells to generate MITO-Tag Mice. Affording spatiotemporal control over MITO-Tag expression, these transgenic animals enable the rapid, cell-type-specific immunoisolation of mitochondria from tissues, which we verified using a combination of proteomic and metabolomic approaches. Using MITO-Tag Mice and targeted and untargeted metabolite profiling, we identified changes during fasted and refed conditions in a diverse array of mitochondrial metabolites in hepatocytes and found metabolites that behaved differently at the mitochondrial versus whole-tissue level. MITO-Tag Mice should have utility for studying mitochondrial physiology, and our strategy should be generally applicable for studying other mammalian organelles in specific cell types in vivo.

scholarly journals Phytochemical Analysis of Curcumin from Turmeric by RP-HPLC

2013 ◽  
Vol 25 (2) ◽  
pp. 995-998 ◽  
Author(s):  
K.J. Lee ◽  
S.D. Choi ◽  
J.Y. Ma
Keyword(s):  
Phytochemical Analysis ◽  
Rp Hplc

scholarly journals MITO-Tag Mice enable rapid isolation and multimodal profiling of mitochondria from specific cell types in vivo

2018 ◽  
Author(s):  
Erol Can Bayraktar ◽  
Lou Baudrier ◽  
Ceren Özerdem ◽  
Caroline A. Lewis ◽  
Sze Ham Chan ◽  
...  
Keyword(s):  
Metabolite Profiling ◽  
Cultured Cells ◽  
Transgenic Animals ◽  
Cell Types ◽  
Tissue Level ◽  
Specific Cell ◽  
Mammalian Tissues ◽  
Cell Type Specific ◽  
Untargeted Metabolite Profiling

ABSTRACTMitochondria are metabolic organelles that are essential for mammalian life, but the dynamics of mitochondrial metabolism within mammalian tissues in vivo remains incompletely understood. While whole-tissue metabolite profiling has been useful for studying metabolism in vivo, such an approach lacks resolution at the cellular and subcellular level. In vivo methods for interrogating organellar metabolites in specific cell-types within mammalian tissues have been limited. To address this, we built on prior work in which we exploited a mitochondrially-localized 3XHA epitope-tag (“MITO-Tag”) for the fast isolation of mitochondria from cultured cells to now generate “MITO-Tag Mice.” Affording spatiotemporal control over MITO-Tag expression, these transgenic animals enable the rapid, cell-type-specific immunoisolation of mitochondria from tissues, which we verified using a combination of proteomic and metabolomic approaches. Using MITO-Tag Mice and targeted and untargeted metabolite profiling, we identified changes during fasted and refed conditions in a diverse array of mitochondrial metabolites in hepatocytes and found metabolites that behaved differently at the mitochondrial versus whole-tissue level. MITO-Tag Mice should have utility for studying mitochondrial physiology and our strategy should be generally applicable for studying other mammalian organelles in specific cell-types in vivo.

Preliminary phytochemical analysis and evaluation of antioxidant, cytotoxic and inhibition of lipopolysaccaride - induced NOS (iNOS) expression in BALB/c mice liver by Ziziphus oenoplia Mill. fruit

2017 ◽  
Vol 14 (2) ◽  
Author(s):  
Ramaraj Thirugnanasampandan ◽  
Gunasekar Ramya ◽  
Gunasekaran Bhuvaneswari ◽  
Srinivasan Aravindh ◽  
Suresh Vaishnavi ◽  
...  
Keyword(s):  
Free Radical ◽  
Bioactive Compounds ◽  
Tamil Nadu ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phytochemical Analysis ◽  
Fruit Extract ◽  
Ms Analysis ◽  
Wide Range ◽  
Mice Liver

AbstractBackground:Mill. is an ethnomedicinal plant and its fruit has been traditionally used by Puliar tribes of Anamalai Hills, Tamil Nadu, India to treat various ailments. Phytochemical analysis, antioxidant, cytotoxic and inducible nitric oxide synthase (Methods:To explore bioactive compounds present in the ripened fruits, high-performance thin-layer chromatography (HPTLC) and gas chromatography/mass spectrometry (GC–MS) analysis were done. Free radical scavenging, hepatoprotective, inhibition ofResults:Total flavonoid content of ZOFwas estimated as 69 µg/mg catechin equivalent. HPTLC densitogram confirmed the presence of quercetin and GC–MS analysis showed a total of 16 compounds of 87.66 % with quinic acid as a major compound which accounted for 22.29 %. Free radical-scavenging activity of ethanolic fruit extract was ranged from 160.12 to 650.23 µg/mL. An amount of 1.5 µg lipopolysaccharide (LPS)- induced severe inflammation in BALB/c mice liver, followed by treatment with ethanolic fruit extract of 100 µg concentration, exhibited significant hepatoprotection and reverse transcriptase polymerase (RT-PCR) analysis showed downregulation ofConclusions:Findings of the present study prove that ZOF is a rich source of bioactive compounds with a wide range of pharmacological activities. Hence, consumption of this wild edible fruit will be a cost-effective and easily available natural nutritional source for health protection.

RP-HPLC-based phytochemical analysis and diverse pharmacological evaluation of Quercus floribunda Lindl. ex A. Camus nuts extracts

2019 ◽  
pp. 1-6
Author(s):  
Madiha Ahmed ◽  
Muhammad Adil ◽  
Ihsan-ul- Haq ◽  
Muhammad Khalid Tipu ◽  
Muhammad Qasim ◽  
...  
Keyword(s):  
Phytochemical Analysis ◽  
Pharmacological Evaluation ◽  
Rp Hplc ◽  
Quercus Floribunda
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