scholarly journals Metabolomics approach to investigate the ergogenic effect of Morinda citrifolia L. leaf extract on obese Sprague Dawley rats

2019 ◽  
Vol 31 (2) ◽  
pp. 191-203 ◽  
Author(s):  
Nordiana Abdul Majid ◽  
Azizah Abdul Hamid ◽  
Syafiq Zikri Salleh ◽  
Nazamid Saari ◽  
Faridah Abas ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Ergogenic Effect ◽  
Morinda Citrifolia ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals EFFECT OF HIGH-DOSE MORINDA CITRIFOLIA (MC) LEAF ETHANOLIC EXTRACT ON SWIMMING ENDURANCE IN FEMALE SPRAGUE DAWLEY RATS

2018 ◽  
Vol 80 (5) ◽  
Author(s):  
M. Z. Zainuddin ◽  
Kamisah Y. ◽  
Farida H. ◽  
Faizah O.
Keyword(s):  
Animal Model ◽  
Leaf Extract ◽  
Oral Intake ◽  
Ethanolic Extract ◽  
Morinda Citrifolia ◽  
High Dose ◽  
Physical Fatigue ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Significant Difference

Morinda citrifolia (MC) is a herb that is famous for its various medicinal properties. However, there has been little research on its effect on increased stamina and physical fatigue reduction. The purpose of this study was to determine the effect of high-dose oral intake of 50% ethanol MC leaf extract on exercise performance and physical fatigue in an animal model. Thirty female Sprague-Dawley rats were divided into five groups; Control (C); Sedentary (S); Exercise (EX); MC leaf extract 1000 mg/kg/day (MC); MC leaf extract 1000 mg/kg/day with Exercise (MC+EX). The endurance test showed a significant difference in endurance time between EX and MC groups, whereby intake of MC leaf extract without daily exercise lowered endurance level by 68.75%. These findings correlated with serum fatigue indicator analysis whereby significant increases in CK levels of MC group, and LDH levels in MC and MC+EX groups were noted when compared to other groups. Electron microscopy analysis on cardiac muscle tissue showed ultrastructural injuries in muscle fibers of the EX, MC and MC+EX groups whereas injuries to the gastrocnemius myofibres were not so prominent. Based on the study findings, it can be concluded that the consumption of 1000 mg/kg/day 50% ethanol MC extract disturbed internal physiology and resulted in injury to the muscle tissues thus lowering rats endurance capability. This shows that a high dose has unfavourable effects on an animal model while a lower dose may be more appropriate.

Ergogenic property of Morinda citrifolia L. leaf extract affects energy metabolism in obese Sprague Dawley rats

2021 ◽  
Author(s):  
Syafiq Zikri Salleh ◽  
Azizah Abdul Hamid ◽  
Ahmad Haniff Jaafar ◽  
Nor Diana Abdul Majid ◽  
Nazamid Saari ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Leaf Extract ◽  
Morinda Citrifolia ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals EVALUATION OF ANTIDIARRHEAL ACTIVITY OF ETHANOLIC LEAF EXTRACT OF ERIOBOTRYA JAPONICA LINDL

2018 ◽  
Vol 11 (9) ◽  
pp. 502
Author(s):  
Davie Rexon Kamadyaapa ◽  
Mavuto Masopera Gondwe ◽  
Mathulo Shauli ◽  
Constance Sewani Rusike ◽  
Jehu Iputo
Keyword(s):  
Castor Oil ◽  
Leaf Extract ◽  
Eriobotrya Japonica ◽  
Cytotoxicity Test ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Adrenoceptor Blocker ◽  
Antidiarrheal Activity ◽  
Pre Treatment ◽  
Ethanolic Leaf Extract

Objective: The study was carried out to investigate the antidiarrheal activity of ethanolic leaf extract of Eriobotrya japonica (EEJ) using various models of experimental diarrhea.Methods: Antidiarrheal property of EEJ at 100, 200, and 400 mg/kg/bwt was evaluated using castor oil-induced diarrhea, castor oil-induced enteropooling, and gastrointestinal propulsive models of experimental diarrhea in Sprague Dawley rats of both sexes, weighing 200–250 g. Cytotoxicity test of EEJ was performed using brine shrimp bioassay.Results: Toxicity assay of EEJ showed a lethal concentration value of 1225 μg/ml suggesting non-toxicity. EEJ significantly (p<0.05) and dose-dependently (100, 200 and 400 mg/kg/bwt) inhibited castor oil-induced diarrhea by 38.1%, 76.19%, and 100%, respectively, and enteropooling by 28%, 56%, and 88%, respectively, compared with control. Pre-treatment with yohimbine, α2-adrenoceptor blocker significantly reversed the protective effect of EEJ (400 mg/kg) against castor oil-induced diarrhea and against castor oil-induced enteropooling, suggesting the involvement of α2-adrenoceptors in antidiarrheal property of EEJ. Furthermore, EEJ significantly (p<0.05) and dosedependently (100, 200, and 400 mg/Kg/bwt) inhibited gastrointestinal motility by 28%, 62%, and 83.92%, respectively.Conclusion: The study has demonstrated the antidiarrheal potential of ethanolic leaf extract of EEJ, which may be attributable to its dual antisecretory and antimotility activities probably through activation of the sympathetic α2-adrenergic pathway.

scholarly journals Effects of Aqueous Quassia amara L. (Korales) Leaf Extract on the Cardiovascular and Respiratory Functions of Male Sprague-Dawley Rats

2018 ◽  
Vol 52 (6) ◽  
Author(s):  
Maria Concepcion C. Sison ◽  
Lynn Crisanta R. Panganiban ◽  
Daisy Mae A. Bagaoisan ◽  
Nelia P. Cortes-Maramba
Keyword(s):  
Blood Pressure ◽  
Adult Male ◽  
Leaf Extract ◽  
Sprague Dawley ◽  
Treatment Groups ◽  
Respiratory Flow ◽  
Sprague Dawley Rats ◽  
Parallel Group ◽  
Aqueous Leaf Extract ◽  
Quassia Amara

Objective. To To evaluate potential effects of the aqueous extract of Quassia amara L. leaves on the cardiovascular and respiratory systems of adult male Sprague- Dawley rats. Methods. The cardiovascular and respiratory effects of the Quassia amara L. leaf extract on adult male SpragueDawley rats were assessed using non-invasive blood pressure (NIBP) determination and head-out plethysmography, respectively, in a randomized, parallel group study. Mean observations of blood pressure and heart rate were recorded at different time periods after dosing. Respiratory flow and irritation effects were evaluated using mean observations of respiratory rate (RR), tidal volume (TV), mid-expiratory flow rate (EF50), time of inspiration (TI) and expiration (TE), and time of break (TB) and pause (TP). Results. There were no significant differences among the control and the treatment groups in SBP, DBP and HR parameters. The extract showed statistically significant effect on mean RR by time period (F=2.45, p=0.0234), trends over time of TV among the dose groups (F=2.00, p=0.0202), and EF50 among dose groups ((F=3.11, p=0.0422). However, these did not correlate with the changes in the time of break (TB) and time of pause (TP) which are more sensitive and specific tests for respiratory irritation. Conclusion. Aqueous leaf extract of Quassia appeared to have no significant effects on SBP, DPB, Pulse pressure, and HR. There are no conclusive dose-related respiratory flow or pulmonary irritation effects.

scholarly journals Antiplasmodial and Antipyretic Activity and Safety Evaluation of the Methanolic Leaf Extract of Murraya exotica (L.)

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Arnold Donkor Forkuo ◽  
Kwesi Boadu Mensah ◽  
Elvis Ofori Ameyaw ◽  
Aaron Opoku Antwi ◽  
Nana Kofi Kusi-Boadum ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Antimalarial Activity ◽  
Baker’S Yeast ◽  
Antiplasmodial Activity ◽  
Rodent Models ◽  
Baker's Yeast ◽  
Sprague Dawley ◽  
Antipyretic Activity ◽  
Sprague Dawley Rats ◽  
Murraya Exotica

Background. The increasing mortality and morbidity of malaria in Africa coupled with the recent reports of antimalarial drug resistance reinforces the need for novel antimalarial agents from natural plant products with folkloric use for the disease. Murraya exotica (L.) (Rutaceae) is widely used as an ornamental plant used indigenously to treat fever, cough, and infectious wounds and eliminate pain from injury and trauma. This study was conducted to evaluate extracts of the leaves of Murraya exotica (L.) (Rutaceae) for its safety and antipyretic and antimalarial activity in rodent models. Method. In this study, the Peters 4-day suppressive and curative test in Plasmodium berghei-infected mice was used to demonstrate the antiplasmodial activity of the methanolic leaf extract of Murraya exotica (L.) (MEE). The study also evaluated the subacute toxicity study and the antipyretic activity of MEE on baker’s yeast-induced hyperthermia in rodent models. Results. Murraya exotica (L.) extract demonstrated curative antimalarial activity, with a percentage suppression of 45.84, 64.32±0.33, 56.74±2.16, and 64.61±0.67 at doses of 50, 100, 300, and 600 mg/kg, respectively. In the Peters 4-day suppressive test, MEE at dose 600 mg/kg had the highest chemosuppression (76.02±1.38%) compared with artesunate (2 mg/kg, p.o.) (82.56±0.97%). Subacute oral toxicity studies in Sprague-Dawley rats documented no deaths, with no significant changes in clinical signs, organ weights, and hematological and biochemical parameters. The LD50 of MEE was estimated to be above 1000 mg/kg in Sprague-Dawley rats. All doses of MEE and paracetamol reduced pyrexia in 1 h and 2 h after their administration. The percentage reduction of rectal temperature (TR) for the positive control (paracetamol, 150 mg/kg, p.o.) was 44.36% while the Murraya exotica extract at doses 50 mg/kg, 100 mg/kg, 300 mg/kg, and 600 mg/kg recorded 67.74%, 40.78%, 66.42%, and 59.42%, respectively. Murraya exotica at dose 100 mg/kg exhibited significant reduction (p<0.05) in baker’s yeast-induced pyrexia. Conclusions. The findings in this study show the antipyretic, curative, and suppressive antiplasmodial activity as well as the safety of the methanolic leaf extract of Murraya exotica (L.) supporting its traditional use for malaria and fever.

scholarly journals ACUTE TOXICITY OF CHITOSAN NANOPARTICLES CONTAINING MAHKOTA DEWA (PHALERIA MACROCARPA) LEAF EXTRACT AND ANTI-INFLAMMATORY EFFECTS IN A DEXTRAN SODIUM SULFATE-INDUCED MOUSE MODEL OF ULCERATIVE COLITIS

2018 ◽  
Vol 10 (1) ◽  
pp. 6
Author(s):  
Ari Estuningtyas ◽  
Santi Widiasari ◽  
Kusmardi Kusmardi
Keyword(s):  
Acute Toxicity ◽  
Leaf Extract ◽  
Chitosan Nanoparticles ◽  
Toxicity Testing ◽  
Inflammatory Cells ◽  
Negative Control ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Phaleria Macrocarpa ◽  
Anti Inflammatory

Objective: The plant mahkota dewa (Phaleria macrocarpa) is known to have anti-inflammatory effects. This study aimed to determine whetherchitosan nanoparticles containing mahkota dewa leaf extract would yield superior anti-inflammatory effects in the colon of a mouse model of dextransodium sulfate (DSS)-induced ulcerative colitis, compared with ethanol extract alone after testing the acute toxicities (lethal dose) of both preparations.Methods: For acute toxicity testing, 10 Sprague-Dawley rats were administered 6000 mg/kg body weight (BW) of leaf extract alone or with nanoparticles.Subsequently, mice were divided into the following six groups to determine the anti-inflammatory effects: Untreated, negative control (DSS 2% w/v), leafextract at 12.5 or 25 mg/kg BW, and leaf extract in chitosan nanoparticles at 6.25 or 12.5 mg/kg BW. To induce colitis, DSS (2% w/v) was administeredthrough drinking water for 6 weeks. The anti-inflammatory effect was observed histopathologically by imaging the inflammatory cells of the mice colonwith hematoxylin-eosin (HE) staining.Results: For acute toxicity testing, 10 Sprague-Dawley rats were administered 6000 mg/kg BW of leaf extract alone or with nanoparticles. Subsequently,mice were divided into the following six groups to determine the anti-inflammatory effects: Untreated, negative control (DSS 2% w/v), leaf extract at12.5 or 25 mg/kg BW, and leaf extract in chitosan nanoparticles at 6.25 or 12.5 mg/kg BW. To induce colitis, DSS (1% w/v) was administered throughdrinking water for 6 weeks. The anti-inflammatory effect was observed histopathologically by imaging the inflammatory cells of the mice colon withHE staining.Conclusion: Chitosan nanoparticles containing mahkota dewa leaf extract can be included in the practically non-toxic class of materials. However, anethanol extract of mahkota dewa leaf effectively inhibited DSS-induced inflammation in the mouse colon, regardless of delivery vehicle.

scholarly journals Hepatoprotective Effects of Morinda citrifolia Leaf Extract on Ovariectomized Rats Fed with Thermoxidized Palm Oil Diet: Evidence at Histological and Ultrastructural Level

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
C. L. G. Chong ◽  
F. Hussan ◽  
F. Othman
Keyword(s):  
Palm Oil ◽  
Leaf Extract ◽  
Normal Diet ◽  
Morinda Citrifolia ◽  
Ovariectomized Rats ◽  
Oxidative Stress Marker ◽  
High Dose ◽  
Therapeutic Effects ◽  
Sprague Dawley ◽  
Hepatoprotective Effects

Morinda citrifolia (Rubiaceae) or Noni was previously reported to have leaf with broad therapeutic property whereas the fruit was rarely described as medicinal. Ironically, extensive research and review has been done on the fruit and little was known about the therapeutic activity of the leaf as a medicinal food. The aim of this study was to investigate the therapeutic effects of Morinda citrifolia (MC) ethanolic leaf extract on the hepatic structure and function in postmenopausal rats fed with thermoxidized palm oil (TPO) diet. Thirty eight female Sprague Dawley rats were divided into five groups: sham (Sham), ovariectomized (OVX), ovariectomized and treated with simvastatin 10 mg/kg (OVX+ST), ovariectomized and supplemented with low dose MC 500 mg/kg (OVX+MCLD), and ovariectomized and supplemented with high dose MC 1000 mg/kg (OVX+MCHD). All the ovariectomized groups were fed with TPO diet whereas the Sham group was fed with normal diet. Consumption of TPO diet in postmenopausal rats resulted in obesity, significantly elevated (P<0.05) liver oxidative stress marker; malondialdehyde (MDA), diffuse microvesicular steatosis, and defective mitochondria. Treatment with MC leaf extract prevented hepatic steatosis by significantly increasing (P<0.05) the liver antioxidant enzyme SOD and GPx, significantly increasing (P<0.05) ALP, decreasing liver lipids infiltration, preventing mitochondrial damage, and overall maintaining the normal liver histology and ultrastructure. In conclusion, we provided detailed histological and ultrastructural evidence showing hepatoprotective effects of MC leaf extract through its antioxidant mechanism.

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