Simultaneous separation and determination of praeruptorin A, B and C by micellar electrokinetic chromatography using sodium dodecyl sulphate and sodium cholate as mixed micelles

2018 ◽  
Vol 29 (5) ◽  
pp. 425-431
Author(s):  
Meng Chen ◽  
Ruimiao Chang ◽  
Liying Xu ◽  
Yayun Huang ◽  
Hongfen Zhang ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Micellar Electrokinetic Chromatography ◽  
Mixed Micelles ◽  
Sodium Dodecyl ◽  
Sodium Cholate ◽  
Electrokinetic Chromatography ◽  
Simultaneous Separation

scholarly journals Analysis of Seven Biogenic Amines and Two Amino Acids in Wines Using Micellar Electrokinetic Chromatography

2019 ◽  
Vol 9 (6) ◽  
pp. 1193 ◽  
Author(s):  
Chih-Ying Huang ◽  
Yu-Xian Wang ◽  
Xin-Zhi Wang ◽  
Cho-Chun Hu ◽  
Tai-Chia Chiu
Keyword(s):  
Amino Acids ◽  
Biogenic Amines ◽  
Micellar Electrokinetic Chromatography ◽  
Low Cost ◽  
Background Electrolyte ◽  
Sodium Dodecyl ◽  
Fast Method ◽  
Electrokinetic Chromatography ◽  
Optimal Separation

A low-cost, simple, and fast method utilizing micellar electrokinetic chromatography for the simultaneous determination of seven biogenic amines and two amino acids was developed. A background electrolyte containing 5 mM phosphate buffer (pH 3.7) and 20 mM sodium dodecyl sulfate was used. The optimal separation of nine investigated analytes was achieved in 11 min, with limits of detection (S/N = 3) ranging from 0.11 to 0.61 µM. The linear ranges for all analytes were observed between 0.55 and 10.0 μM (R2 > 0.990). The developed approach was extended to the analysis of analytes in commercial wine and beer samples. The recoveries of the proposed method ranged from 98.8% to 115.6%.

scholarly journals A fast and efficient method for the study of caffeine levels in energy drinks using micellar electrokinetic chromatography (MEKC)

2012 ◽  
Vol 32 (2) ◽  
pp. 401-404 ◽  
Author(s):  
Cristiano Augusto Ballus ◽  
Adriana Dillenburg Meinhart ◽  
Carolina Schaper Bizzotto ◽  
José Teixeira Filho ◽  
Helena Teixeira Godoy
Keyword(s):  
Micellar Electrokinetic Chromatography ◽  
Brain Activity ◽  
Low Cost ◽  
Sodium Dodecyl ◽  
Energy Drinks ◽  
Electrokinetic Chromatography ◽  
The Central Nervous System ◽  
The Mean ◽  
High Doses

Energy drinks are becoming popular in Brazil and in the world due to their stimulant properties. Caffeine is present in energy drinks with the aim of stimulating the central nervous system and intensifying brain activity. On the other hand, the ingestion of high doses of caffeine can cause undesirable symptoms such as anxiety and tachycardia. Therefore, it is necessary to monitor the caffeine content added to energy drinks to guarantee that the levels in the final product are in accordance with the labeling and within the legislation limits. The goal of this work was to validate a fast, efficient, and low-cost method for the determination of caffeine in energy drinks by micellar electrokinetic chromatography (MEKC). A total of seven brands were analyzed, each in three lots. The electrolyte was prepared with 50 mmol.L-1 of sodium dodecyl sulfate (SDS) and 10 mmol.L-1 of sodium carbonate (pH 11.0). The mean concentration of caffeine ranged from 122.8 to 318.6 mg.L-1. None of the brands had caffeine levels above the maximum limit. Considering the interval of confidence (95%), 72% of the samples had less caffeine than the amount informed on the product label.

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