Sensitive and Selective Determination of Phenolic Compounds from Aromatic Plants Using an Electrochemical Detection Coupled with HPLC Method

2014 ◽  
Vol 25 (3) ◽  
pp. 247-254 ◽  
Author(s):  
Alberto Cantalapiedra ◽  
Mª Jesús Gismera ◽  
Mª Teresa Sevilla ◽  
Jesús R. Procopio
Keyword(s):  
Phenolic Compounds ◽  
Electrochemical Detection ◽  
Hplc Method ◽  
Aromatic Plants ◽  
Selective Determination

Selective Determination of Entecavir in the Presence of Its Oxidative Degradate by Spectrophotometric and Chromatographic Methods

2021 ◽  
Author(s):  
Heba M El-Sayed ◽  
Laila E Abdel Fattah ◽  
Hisham E Abdellatef ◽  
Maha A Hegazy ◽  
Mai M Abd El-Aziz
Keyword(s):  
High Selectivity ◽  
Oxidative Degradation ◽  
Hplc Method ◽  
Peak Amplitude ◽  
Quality Analysis ◽  
Official Method ◽  
Selective Determination ◽  
Significant Difference ◽  
Development And Validation

Abstract Background Entecavir (ENT) is an antiretroviral agent prescribed for treatment of HBV and HIV. Objective Development and validation of three simple, sensitive, selective, and precise methods for determination of ENT in presence of its oxidative degradation product (ENT deg.). Methods The first method was based on second derivative (D2) spectrophotometry through measuring the peak amplitude of D2 spectra at 293.6 nm. The second one is mean centering of the ratio spectra (MCR), which allowed measuring the peak amplitude at 280.0 nm. While the third method was HPLC; where ENT was separated from ENT deg. using Zobrax C18column and methanol: water (30:70, v/v), pH 3 as a mobile phase. The three developed methods were validated according to ICH guidelines. Results Linearity range of ENT was 5.00–50.00 μg/mL for both D2and MCR. However, higher sensitivity was achieved using HPLC (1.00–50.00 μg/mL). Accuracy of ENT were 100.60%±0.547, 101.55%±1.2071 and 100.61%±1.207 for D2, MCR and HPLC methods, respectively, and precision was within 1.280. Conclusions The developed methods were successfully applied for the determination of ENT in Tecavir® tablets without interference from ENT deg. They showed no significant difference compared with the official method as well as they could be applied in the quality analysis of ENT with high selectivity, accuracy, and precision. Highlights ENT was quantified using two spectrophotometric (D2 and MCR) methods and an HPLC method in presence of ENT deg. The proposed methods were applied to analysis of ENT tablets with high selectivity, sensitivity, and accuracy.

scholarly journals Membrane Sensors for the Selective Determination of Donepezil Hydrochloride

2010 ◽  
Vol 93 (2) ◽  
pp. 549-555 ◽  
Author(s):  
Gamal Abdel Hafiz Mostafa ◽  
Mohamed Hefnawy ◽  
Abdulrahman Al-Majed
Keyword(s):  
Direct Determination ◽  
Pharmaceutical Preparations ◽  
Ion Association ◽  
Phosphomolybdic Acid ◽  
Hplc Method ◽  
Pvc Membrane ◽  
Selective Determination ◽  
Response Characteristics ◽  
Sodium Tetraphenyl Borate

Abstract The construction and electrochemical response characteristics of polyvinylchloride (PVC) membrane sensors for donepezil HCl (DP) are described. The sensing membranes incorporated ion-association complexes of DP cation and sodium tetraphenyl borate (sensor 1), phosphomolybdic acid (sensor 2), or phosphotungstic acid (sensor 3) as electroactive materials. The sensors displayed a fast, stable, and near-Nernstian response over a relatively wide DP concentration range (1 102 to 1 106 M), with cationic slopes of 53.0, 54.0, and 51.0 mV/ concentration decade over a pH range of 4.0 to 8.0. The sensors showed good discrimination of DP from several inorganic and organic compounds. The direct determination of 2.54000.0 g/mL DP showed average recoveries of 99.0, 99.5, and 98.5, and mean RSDs of 1.6, 1.5, and 1.7 at 100.0 g/mL for sensors 1, 2, and 3, respectively. The proposed sensors have been applied for direct determination of DP in two pharmaceutical preparations. The results obtained for determination of DP in tablets using the proposed sensors compared favorably with those obtained using an HPLC method. The sensors have been used as indicator electrodes for potentiometric titration of DP.

scholarly journals A facile molecularly imprinted electrochemical sensor based on graphene: application to the selective determination of thiamethoxam in grain

RSC Advances ◽  
2017 ◽  
Vol 7 (62) ◽  
pp. 38884-38894 ◽  
Author(s):  
Tianjiao Xie ◽  
Min Zhang ◽  
Pan Chen ◽  
Haitian Zhao ◽  
Xin Yang ◽  
...  
Keyword(s):  
Electrochemical Sensor ◽  
Electrochemical Detection ◽  
Molecularly Imprinted Polymer ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Selective Determination

In this study, we report a facile method for the preparation of molecularly imprinted polymer based graphene for the electrochemical detection of thiamethoxam residue.

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