One- and Two-dimensional High-performance Thin-layer Chromatography as an Alternative Analytical Tool for Investigating Polyphenol-Protein Interactions

2013 ◽  
Vol 24 (5) ◽  
pp. 436-445 ◽  
Author(s):  
Kathrin Tscherch ◽  
Julia Biller ◽  
Mareen Lehmann ◽  
Maria Trusch ◽  
Sascha Rohn
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Protein Interactions ◽  
High Performance ◽  
Analytical Tool ◽  
Two Dimensional ◽  
Layer Chromatography

High Performance Thin Layer Chromatography: A Mini Review

2016 ◽  
Vol 2 (4) ◽  
pp. 219-226
Author(s):  
S. V. Saibaba ◽  
P. Shanmuga Pandiyan
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Separation Efficiency ◽  
Analytical Tool ◽  
Combinatorial Approach ◽  
Herbal Drug ◽  
Biomedical Analysis ◽  
Layer Chromatography

High Performance Thin layer Chromatography (HPTLC) technique is a sophisticated and automated form of the thin-layer chromatography (TLC) with better and advanced separation efficiency and detection limits, and is often an excellent alternative to GC and HPLC. Applications of HPTLC include phytochemical and biomedical analysis, herbal drug quantification, active ingredient quantification, fingerprinting of formulations, and check for adulterants in the formulations. HPTLC is useful in detecting chemicals of forensic concern. Various advance techniques in reference to HPTLC like hyphenations in HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser have made HPTLC a power analytical tool in the field of analysis. Experts are of the opinion that HPTLC future to combinatorial approach and the utilization of instrumental HPTLC toward the analysis of drug formulations, bulk drugs, and natural products will increase in the future

scholarly journals Application of High-Performance, Thin-Layer Chromatography to Quality Control of Antimetabolite Analogue Infusion Bags

2001 ◽  
Vol 84 (4) ◽  
pp. 1296-1300 ◽  
Author(s):  
Laurent Perello ◽  
Sylvie Demirdjian ◽  
Alexandre Dory ◽  
Philippe Bourget
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Double Standard ◽  
Analytical Tool ◽  
Quality Program ◽  
Chemotherapy Infusion ◽  
Standard Calibration ◽  
Layer Chromatography

Abstract A global postproduction quality program was developed to secure chemotherapy infusion at the Gustave Roussy Institute. Despite rigorous procedures and computerized prescriptions, an analytical check was necessary to improve the quality of ready-to-use solutions of cytotoxic drugs in our Centralized Antineoplastics Reconstitution Unit. High-performance, thin-layer chromatography was selected as the analytical tool to assay 12 anticancer drugs. One of the analytical methods can separate 4 antimetabolite substances, i.e., fludarabine (FDB), cytarabine (CTB), gemcitabine (GTB), and fluorouracil (5 FU). For all infusion bags manufactured, up to 26 samples could be assayed per series using a double standard calibration (GTB and 5 FU).

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