Prevention and monitoring of invasive fungal infections in pediatric patients with cancer and hematologic disorders

2006 ◽  
Vol 48 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Liisa Hovi ◽  
Harri Saxen ◽  
Ulla M. Saarinen-Pihkala ◽  
Kim Vettenranta ◽  
Taru Meri ◽  
...  
Keyword(s):  
Pediatric Patients ◽  
Fungal Infections ◽  
Invasive Fungal Infections ◽  
Hematologic Disorders ◽  
Patients With Cancer

scholarly journals 113 Invasive fungal infections among pediatric patients with hematologic malignancies at KFSH&RC/KFCCC&R

2006 ◽  
Vol 10 ◽  
pp. S63
Author(s):  
I. Bin-Hussain ◽  
F. Al Kordy ◽  
R. Serhan ◽  
A. Al Ahmari ◽  
A. Belgaumi ◽  
...  
Keyword(s):  
Pediatric Patients ◽  
Fungal Infections ◽  
Hematologic Malignancies ◽  
Invasive Fungal Infections

scholarly journals Non-Invasive Methods to Diagnose Fungal Infections in Pediatric Patients with Hematologic Disorders

2016 ◽  
Vol 9 (11) ◽  
Author(s):  
Parisa Badiee ◽  
Zahra Hashemizadeh ◽  
Mani Ramzi ◽  
Mohammad Karimi ◽  
Rasoul Mohammadi
Keyword(s):  
Pediatric Patients ◽  
Fungal Infections ◽  
Hematologic Disorders ◽  
Non Invasive

scholarly journals 78. Non-Invasive Prediction of Invasive Fungal Infection by Plasma-Based Microbial Cell-Free DNA Next-Generation Sequencing (mcfDNA NGS) in Pediatric Patients with Relapsed or Refractory Leukemia

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S51-S51
Author(s):  
Joshua Wolf ◽  
Joshua Wolf ◽  
Gabriela Maron ◽  
Kathryn Goggin ◽  
Kim J Allison ◽  
...  
Keyword(s):  
Fungal Infection ◽  
Invasive Fungal Infection ◽  
Fungal Pathogen ◽  
Pediatric Patients ◽  
Hematopoietic Cell Transplantation ◽  
Fungal Infections ◽  
Invasive Fungal Infections ◽  
Non Invasive ◽  
Clinical Onset ◽  
Fungal Dna

Abstract Background Diagnosis of invasive fungal infections (IFIs), a life-threatening complication of cancer therapy or hematopoietic cell transplantation (HCT) can be challenging, and IFI has poor outcomes. Prediction or early non-invasive diagnosis of IFI in high-risk hosts before onset of symptoms could reduce morbidity and mortality. Because non-invasive plasma mcfDNA NGS can detect invasive fungal infections, and may predict bloodstream infections in immunocompromised patients, we hypothesized that mcfDNA NGS might also predict invasive fungal infection before clinical presentation. Methods In a prospective study, serial remnant plasma samples were collected from pediatric patients undergoing treatment for relapsed or refractory leukemia. IFI events were classified according to EORTC criteria by 2 independent experts, and episodes empirically treated for suspected IFI, but not meeting ‘possible’ criteria were classified as ‘suspected’. All samples collected within 30 days before clinical diagnosis of non-fungemic IFI were tested for fungal DNA by mcfDNA NGS using a research-use only assay by Karius, Inc. optimized for fungi; because of overlapping clinical syndromes, non-fungal DNA was not considered in this study. Results There were 15 episodes of suspected IFI in 14 participants with ≥1 sample available from either diagnostic (within 1 day of diagnosis) or predictive (2 to 30 days prior to diagnosis) periods (5 “suspected”, and 4 probable and 6 proven by EORTC definitions). Of 10 probable or proven IFIs, 6 (60%) had a relevant fungal pathogen identified mcfDNA NGS at diagnosis. In each of these cases the fungal DNA was also detectable prior to clinical onset of IFI (Range 2 to 41 days; Figure 1). In an additional case, manual review of sequence data identified the fungal DNA at diagnosis and during the prior month. Of 5 “suspected” IFI episodes, all were determined by expert review as not representing fungal infection; fungal DNA was identified by mcfDNA NGS in 2/54 (3.7%) of samples from these episodes. Table 1. Characteristics of Invasive Fungal Infections Conclusion mcfDNA NGS can identify fungal pathogen DNA before clinical onset of IFI, so might predict IFI in immunocompromised hosts, and may help differentiate fungal infection from other etiologies of lung nodules or infiltrates. Disclosures Joshua Wolf, MBBS, PhD, FRACP, Karius Inc. (Research Grant or Support) Joshua Wolf, MBBS, PhD, FRACP, Nothing to disclose Radha Duttagupta, PhD, Karius inc (Employee) Lily Blair, PhD, Karius Inc. (Employee) Asim A. Ahmed, MD, Karius, Inc. (Employee)

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