Ductal epithelial proliferations of the breast: a biological continuum? Comparative genomic hybridization and high-molecular-weight cytokeratin expression patterns

2001 ◽  
Vol 195 (4) ◽  
pp. 415-421 ◽  
Author(s):  
Werner Boecker ◽  
Horst Buerger ◽  
Klaus Schmitz ◽  
Ian A. Ellis ◽  
Paul J. van Diest ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Comparative Genomic Hybridization ◽  
Expression Patterns ◽  
Comparative Genomic ◽  
Genomic Hybridization ◽  
Cytokeratin Expression

scholarly journals Molecular features and vulnerabilities of recurrent chordomas

2021 ◽  
Vol 40 (1) ◽  
Author(s):  
Carolin Seeling ◽  
André Lechel ◽  
Michael Svinarenko ◽  
Peter Möller ◽  
Thomas F. E. Barth ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Comparative Genomic Hybridization ◽  
Cell Lines ◽  
Array Comparative Genomic Hybridization ◽  
Expression Patterns ◽  
Comparative Genomic ◽  
Genomic Hybridization ◽  
Culture Models ◽  
Cell Culture Models

Abstract Background Tumor recurrence is one of the major challenges in clinical management of chordoma. Despite R0-resection, approximately 50% of chordomas recur within ten years after initial surgery. The underlying molecular processes are poorly understood resulting in the lack of associated therapeutic options. This is not least due to the absence of appropriate cell culture models of this orphan disease. Methods The intra-personal progression model cell lines U-CH11 and U-CH11R were compared using array comparative genomic hybridization, expression arrays, RNA-seq, and immunocytochemistry. Cell line origin was confirmed by short tandem repeat analysis. Inter-personal cell culture models (n = 6) were examined to validate whether the new model is representative. Cell viability after HOX/PBX complex inhibition with small peptides was determined by MTS assays. Results Using whole genome microarray analyses, striking differences in gene expression between primary and recurrent chordomas were identified. These expression differences were confirmed in the world’s first intra-personal model of chordoma relapse consisting of cell lines established from a primary (U-CH11) and the corresponding recurrent tumor (U-CH11R). Array comparative genomic hybridization and RNA-sequencing analyses revealed profound genetic similarities between both cell lines pointing to transcriptomic reprogramming as a key mechanism of chordoma progression. Network analysis of the recurrence specific genes highlighted HOX/PBX signaling as a common dysregulated event. Hence, HOX/PBX complexes were used as so far unknown therapeutic targets in recurrent chordomas. Treating chordoma cell lines with the complex formation inhibiting peptide HXR9 induced cFOS mediated apoptosis in all chordoma cell lines tested. This effect was significantly stronger in cell lines established from chordoma relapses. Conclusion Clearly differing gene expression patterns and vulnerabilities to HOX/PBX complex inhibition in highly therapy resistant chordoma relapses were identified using the first intra-personal loco-regional and further inter-personal chordoma progression models. For the first time, HOX/PBX interference was used to induce cell death in chordoma and might serve as the basic concept of an upcoming targeted therapy for chordomas of all progression stages.

566: Comparative Genomic Hybridization of Chemotherapy Survived Wilms' Tumor Cells

2004 ◽  
Vol 171 (4S) ◽  
pp. 150-151
Author(s):  
Thorsten Schlomm ◽  
Bastian Gunawan ◽  
Hans J. Schulten ◽  
Norbert Graf ◽  
Ivo Leuschner ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Comparative Genomic Hybridization ◽  
Wilms Tumor ◽  
Comparative Genomic ◽  
Genomic Hybridization
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