Somatic copy number alterations by whole-exome sequencing implicates YWHAZ and PTK2 in castration-resistant prostate cancer. J Pathol 2013; 231: 505-516

2014 ◽  
Vol 233 (1) ◽  
pp. 101-101 ◽  
Keyword(s):  
Prostate Cancer ◽  
Exome Sequencing ◽  
Whole Exome Sequencing ◽  
Copy Number ◽  
Copy Number Alterations ◽  
Castration Resistant Prostate Cancer ◽  
Castration Resistant ◽  
Whole Exome ◽  
Somatic Copy Number Alterations

Integrative Exome Sequencing Analysis in Castration-Resistant Prostate Cancer in Chinese Population

2020 ◽  
Vol 21 (2) ◽  
pp. 140-148
Author(s):  
Lifang Hao ◽  
Hui Li ◽  
Su Zhang ◽  
Yanlei Yang ◽  
Zhenzhen Xu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Exome Sequencing ◽  
Molecular Mechanism ◽  
Whole Exome Sequencing ◽  
Ffpe Tissue ◽  
Sequencing Analysis ◽  
Castration Resistant Prostate Cancer ◽  
Castration Resistant ◽  
Phosphorylation Level ◽  
Whole Exome

Background: Castration-resistant Prostate Cancer (CRPC) is a fatal disease with rapid growth. The malignancy usually presents with metastasis and poor prognosis, and causes 100% mortality. Therefore, the treatment of CRPC is extremely challenging, and its pathogenesis need to be elucidated urgently. Objective: The high throughput sequencing technology was used to sequence the whole exome associated with CRPC, to explore the molecular mechanism of CRPC, and to find the potential therapeutic targets. Methods: We performed whole-exome sequencing of FFPE tissue from 11 Chinese adult male patients. Genomic DNA was fragmented and enriched for whole-exome sequencing using the QiAamp DNA FFPE Tissue KIT, sequenced on an Illumina HiSeq2000 platform, and the relevant genes were analyzed using biological information. Finally, immunohistochemistry method was used to detect the phosphorylation level of LATS1 in CRPC tissues of MST1 mutant and non-mutant patients. Results: We have screened 85 significant mutant genes with relatively high mutation rates of TP53, AR, KMT2, DMAPK1, PIK3R1, SH2B3, WHSC1, KMT2D, MST1 and MAPK1. We first found that MST1 has multiple mutations in CRPC patients, and the MST1 plays an important role in the Hippo pathway. Immunohistochemistry results showed that the phosphorylation level of LATS1 in the mutant patients was significantly lower than that in the non-mutant patients. Conclusion: We speculate that MST1 would be a new potential target for the treatment of CRPC by regulating Hippo signaling pathway. The results provided an important clue to the molecular mechanism of CRPC.

Abstract C27: Whole-exome sequencing identifies potential therapeutic targets for castration-resistant prostate cancer

2012 ◽  
Vol 72 (4 Supplement) ◽  
pp. C27-C27
Author(s):  
Roopika Menon ◽  
Mario Deng ◽  
Diana Boehm ◽  
Falko Fend ◽  
Detlef Boehm ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Exome Sequencing ◽  
Whole Exome Sequencing ◽  
Therapeutic Targets ◽  
Castration Resistant Prostate Cancer ◽  
Castration Resistant ◽  
Whole Exome

scholarly journals Whole-Exome Sequencing Characterizes the Landscape of Somatic Mutations and Copy Number Alterations in Adrenocortical Carcinoma

2015 ◽  
Vol 100 (3) ◽  
pp. E493-E502 ◽  
Author(s):  
C. Christofer Juhlin ◽  
Gerald Goh ◽  
James M. Healy ◽  
Annabelle L. Fonseca ◽  
Ute I. Scholl ◽  
...  
Keyword(s):  
Exome Sequencing ◽  
Whole Exome Sequencing ◽  
Adrenocortical Carcinoma ◽  
Copy Number ◽  
Somatic Mutations ◽  
Copy Number Alterations ◽  
Whole Exome
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