The pathological roles of BART miRNAs in nasopharyngeal carcinoma

2012 ◽  
Vol 227 (4) ◽  
pp. 392-403 ◽  
Author(s):  
Angela K-F Lo ◽  
Christopher W Dawson ◽  
Dong-Yan Jin ◽  
Kwok-Wai Lo
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Bart Mirnas

scholarly journals NF-κB Signaling Regulates Expression of Epstein-Barr Virus BART MicroRNAs and Long Noncoding RNAs in Nasopharyngeal Carcinoma

2016 ◽  
Vol 90 (14) ◽  
pp. 6475-6488 ◽  
Author(s):  
Rob J. A. Verhoeven ◽  
Shuang Tong ◽  
Gaohong Zhang ◽  
Jingfeng Zong ◽  
Yixin Chen ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Noncoding Rnas ◽  
Viral Proteins ◽  
Long Noncoding Rnas ◽  
Barr Virus ◽  
Iκb Kinase ◽  
Lmp1 Expression ◽  
Epstein Barr ◽  
Bart Mirnas

ABSTRACTEpstein-Barr virus (EBV) expresses few viral proteins in nasopharyngeal carcinoma (NPC) but high levels of BamHI-A rightward transcripts (BARTs), which include long noncoding RNAs (lncRNAs) and BART microRNAs (miRNAs). It is hypothesized that the mechanism for regulation of BARTs may relate to EBV pathogenesis in NPC. We showed that nuclear factor-κB (NF-κB) activates the BART promoters and modulates the expression of BARTs in EBV-infected NPC cells but that introduction of mutations into the putative NF-κB binding sites abolished activation of BART promoters by NF-κB. Binding of p50 subunits to NF-κB sites in the BART promoters was confirmed in electrophoretic mobility shift assays (EMSA) and further demonstratedin vivousing chromatin immunoprecipitation (ChIP) analysis. Expression of BART miRNAs and lncRNAs correlated with NF-κB activity in EBV-infected epithelial cells, while treatment of EBV-harboring NPC C666-1 cells with aspirin (acetylsalicylic acid [ASA]) and the IκB kinase inhibitor PS-1145 inhibited NF-κB activity, resulting in downregulation of BART expression. Expression of EBV LMP1 activates BART promoters, whereas an LMP1 mutant which cannot induce NF-κB activation does not activate BART promoters, further supporting the idea that expression of BARTs is regulated by NF-κB signaling. Expression of LMP1 is tightly regulated in NPC cells, and this study confirmed that miR-BART5-5p downregulates LMP1 expression, suggesting a feedback loop between BART miRNA and LMP1-mediated NF-κB activation in the NPC setting. These findings provide new insights into the mechanism underlying the deregulation of BARTs in NPC and identify a regulatory loop through which BARTs support EBV latency in NPC.IMPORTANCENasopharyngeal carcinoma (NPC) cells are ubiquitously infected with Epstein-Barr virus (EBV). Notably, EBV expresses very few viral proteins in NPC cells, presumably to avoid triggering an immune response, but high levels of EBV BART miRNAs and lncRNAs which exhibit complex functions associated with EBV pathogenesis. The mechanism for regulation of BARTs is critical for understanding NPC oncogenesis. This study provides multiple lines of evidence to show that expression of BARTs is subject to regulation by NF-κB signaling. EBV LMP1 is a potent activator of NF-κB signaling, and we demonstrate that LMP1 can upregulate expression of BARTs through NF-κB signaling and that BART miRNAs are also able to downregulate LMP1 expression. It appears that aberrant NF-κB signaling and expression of BARTs form an autoregulatory loop for maintaining EBV latency in NPC cells. Further exploration of how targeting NF-κB signaling interrupts EBV latency in NPC cells may reveal new options for NPC treatment.

An Established Epithelial Cell Line (NPC/HK1) from a Nasopharyngeal Carcinoma - II. A Sem Study

1982 ◽  
Vol 40 ◽  
pp. 224-225
Author(s):  
Li C.L. ◽  
Chew E.C. ◽  
Huang D.P. ◽  
Ho H.C. ◽  
Mak L.S. ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cell ◽  
Surface Morphology ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Cells In Culture ◽  
Sem Study ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.

Studies of Epstein-Barr Virus Antigens Using Immunoperoxidase and Immunofluorescence Techniques

1975 ◽  
Vol 33 ◽  
pp. 342-343
Author(s):  
R. Stephens ◽  
K. Traul ◽  
D. Woolf ◽  
P. Gaudreau
Keyword(s):  
Electron Microscopy ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
If Technique ◽  
Infected Cells ◽  
Virus Antigens ◽  
Epstein Barr ◽  
Virus Capsid Antigen ◽  
Antigen Reactivity

A number of antigens have been found associated with persistent EBV infections of lymphoblastoid cells. Identification and localization of these antigens were principally by immunofluorescence (IF) techniques using sera from patients with nasopharyngeal carcinoma (NPC), Burkitt lymphoma (BL), and infectious mononucleosis (IM). Our study was mainly with three of the EBV related antigens, a) virus capsid antigen (VCA), b) membrane antigen (MA), and c) early antigens (EA) using immunoperoxidase (IP) techniques with electron microscopy (EM) to elucidate the sites of reactivity with EBV and EBV infected cells.Prior to labeling with horseradish peroxidase (HRP), sera from NPC, IM, and BL cases were characterized for various reactivities by the indirect IF technique. Modifications of the direct IP procedure described by Shabo and the indirect IP procedure of Leduc were made to enhance penetration of the cells and preservation of antigen reactivity.

A Phase II Trial of Proton Radiotherapy with Chemotherapy for Nasopharyngeal Carcinoma

2014 ◽  
Vol 75 (S 02) ◽  
Author(s):  
Annie Chan ◽  
E. Weyman ◽  
T. Goldsmith ◽  
L. Wirth ◽  
J. Clark ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Phase Ii ◽  
Phase Ii Trial ◽  
Proton Radiotherapy

Sinonasal Morbidity Following Chemoradiation in Nasopharyngeal Carcinoma Patients

2020 ◽  
Author(s):  
Matthew Stewart ◽  
Chandala Chitguppi ◽  
Kira Murphy ◽  
Tawfiq Koury ◽  
Mackenzie O'Connor ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma

Painful ophthalmoplegia secondary to nasopharyngeal carcinoma: a case report

1990 ◽  
Vol 4 (2) ◽  
pp. 112
Author(s):  
Young Bae Roh ◽  
Jhoon Ho Kim ◽  
Joo Young Song ◽  
Boo Sup Oum
Keyword(s):  
Case Report ◽  
Nasopharyngeal Carcinoma ◽  
Painful Ophthalmoplegia

Two Cases of Pediatric Nasopharyngeal Carcinoma

2009 ◽  
Vol 52 (10) ◽  
pp. 845
Author(s):  
Joon Kyoo Lee ◽  
Hyun Seok Choi ◽  
Dae Woong Lee ◽  
Sung Woo Cho
Keyword(s):  
Nasopharyngeal Carcinoma
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