Comprehensive genetic and functional characterization of IPH-926: a novelCDH1-null tumour cell line from human lobular breast cancer

2009 ◽  
Vol 217 (5) ◽  
pp. 620-632 ◽  
Author(s):  
Matthias Christgen ◽  
Henriette Bruchhardt ◽  
Catarina Hadamitzky ◽  
Cornelia Rudolph ◽  
Doris Steinemann ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Tumour Cell ◽  
Functional Characterization ◽  
Tumour Cell Line ◽  
Lobular Breast Cancer

scholarly journals Characterization of human foetal intestinal alkaline phosphatase. Comparison with the isoenzymes from the adult intestine and human tumour cell lines

1983 ◽  
Vol 211 (3) ◽  
pp. 553-558 ◽  
Author(s):  
C M Behrens ◽  
C A Enns ◽  
H H Sussman
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Line ◽  
Tumour Cell ◽  
Tumour Cell Line ◽  
Subunit Structure ◽  
Human Tumour ◽  
Intestinal Alkaline Phosphatase ◽  
Alkaline Phosphatases ◽  
Inhibition Studies

The molecular structure of human foetal intestinal alkaline phosphatase was defined by high-resolution two-dimensional polyacrylamide-gel electrophoresis and amino acid inhibition studies. Comparison was made with the adult form of intestinal alkaline phosphatase, as well as with alkaline phosphatases isolated from cultured foetal amnion cells (FL) and a human tumour cell line (KB). Two non-identical subunits were isolated from the foetal intestinal isoenzyme, one having same molecular weight and isoelectric point as placental alkaline phosphatase, and the other corresponding to a glycosylated subunit of the adult intestinal enzyme. The FL-cell and KB-cell alkaline phosphatases were also found to contain two subunits similar to those of the foetal intestinal isoenzyme. Characterization of neuraminidase digests of the non-placental subunit showed it to be indistinguishable from the subunits of the adult intestinal isoenzyme. This implies that no new phosphatase structural gene is involved in the transition from the expression of foetal to adult intestinal alkaline phosphatase, but that the molecular changes involve suppression of the placental subunit and loss of neuraminic acid from the non-placental subunit. Enzyme-inhibition studies demonstrated an intermediate response to the inhibitors tested for the foetal intestinal, FL-cell and KB-cell isoenzymes when compared with the placental, adult intestinal and liver forms. This result is consistent with the mixed-subunit structure observed for the former set of isoenzymes. In summary, this study has defined the molecular subunit structure of the foetal intestinal form of alkaline phosphatase and has demonstrated its expression in a human tumour cell line.

Establishment and Characterization of a Novel Primitive Yolk Sac Tumour Cell Line, TC587

2020 ◽  
Vol 40 (2) ◽  
pp. 759-766 ◽  
Author(s):  
TAKESHI IWASAKI ◽  
KENICHI KOHASHI ◽  
MASASUKE OHNO ◽  
TOMOAKI TAGUCHI ◽  
YOSHINAO ODA
Keyword(s):  
Cell Line ◽  
Tumour Cell ◽  
Yolk Sac ◽  
Tumour Cell Line ◽  
Yolk Sac Tumour

Immortalization and characterization of a new canine mammary tumour cell line FR37-CMT

2016 ◽  
Vol 15 (3) ◽  
pp. 952-967 ◽  
Author(s):  
L. R. Raposo ◽  
C. Roma-Rodrigues ◽  
P. Faísca ◽  
M. Alves ◽  
J. Henriques ◽  
...  
Keyword(s):  
Cell Line ◽  
Tumour Cell ◽  
Mammary Tumour ◽  
Tumour Cell Line ◽  
Canine Mammary Tumour

Derivation and characterization of a Wilms' tumour cell line, WiT 49

2003 ◽  
Vol 107 (3) ◽  
pp. 365-374 ◽  
Author(s):  
Jennifer Alami ◽  
Bryan R. Williams ◽  
Herman Yeger
Keyword(s):  
Cell Line ◽  
Tumour Cell ◽  
Tumour Cell Line ◽  
Wilms Tumour
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