Diagnostic and prognostic significance of clonal T-cell receptor beta gene rearrangements in lymph nodes of patients with mycosis fungoides

1993 ◽  
Vol 170 (3) ◽  
pp. 249-255 ◽  
Author(s):  
Victor Bakels ◽  
Johan W. Van Oostveen ◽  
Marie-Louise Geerts ◽  
Roel L. J. Gordin ◽  
Jan M. M. Walboomers ◽  
...  
Keyword(s):  
T Cell ◽  
Lymph Nodes ◽  
Mycosis Fungoides ◽  
T Cell Receptor ◽  
Prognostic Significance ◽  
Cell Receptor ◽  
Gene Rearrangements ◽  
T Cell Receptor Beta ◽  
Beta Gene ◽  
Receptor Beta

Unexpected consistent involvement of V beta gene segments in inappropriate T-cell receptor beta gene rearrangements occurring in B- lineage acute lymphoblastic leukemias

Blood ◽  
1992 ◽  
Vol 80 (10) ◽  
pp. 2614-2621 ◽  
Author(s):  
H Dombret ◽  
P Loiseau ◽  
JC Bories ◽  
F Sigaux
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Genomic Library ◽  
Cell Receptor ◽  
Gene Rearrangements ◽  
Specific Pcr ◽  
T Cell Receptor Beta ◽  
B Lineage ◽  
Beta Gene ◽  
Receptor Beta

Abstract T-cell receptor beta (TCR beta) gene rearrangements occur in a third of early B-cell acute lymphoblastic leukemias (ALLs). V, D, and J segments involved in these inappropriate rearrangements remain unknown and are of interest, both because partial D beta J beta and complete V beta D beta J beta recombinations occur at distinct stages of thymic maturation and because these rearrangements are regulated differently. We have therefore studied in detail seven cases of B-lineage ALL that show inappropriate clonal TCR beta gene rearrangements. Analysis of genomic DNA by Southern hybridization with C beta, J beta 1, V beta 8, and V beta 11 probes suggested the involvement of V beta segment in tumor cell rearrangements. A complete genomic library constructed from one case was screened with a C beta probe, and the TCR beta gene rearrangement was cloned and fully sequenced to show an out of frame V beta 2.2-J beta 2.6 recombination. TCR beta gene rearrangements occurring in other cases were further analyzed by polymerase chain reaction (PCR) using J beta and V beta primers and the resulting specific PCR products were sequenced. Evidence of clonal V beta rearrangements was obtained in all cases. These unexpected findings represent the first definitive demonstration that complete V beta(D beta)J beta rearrangements can occur in B-lineage cells and contrast with the previously reported lack of V beta(D beta)J beta rearrangement in B cells from V beta-J beta-C beta-E mu transgenic mice. In the context of increasing evidence that rearrangements are linked to transcription of unrearranged gene segments, these data prompt a search in B-lineage ALL cells for the presence of germline V beta transcripts whose deregulated expression may be linked to early transforming events.

scholarly journals Unexpected consistent involvement of V beta gene segments in inappropriate T-cell receptor beta gene rearrangements occurring in B- lineage acute lymphoblastic leukemias

Blood ◽  
1992 ◽  
Vol 80 (10) ◽  
pp. 2614-2621
Author(s):  
H Dombret ◽  
P Loiseau ◽  
JC Bories ◽  
F Sigaux
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Genomic Library ◽  
Cell Receptor ◽  
Gene Rearrangements ◽  
Specific Pcr ◽  
T Cell Receptor Beta ◽  
B Lineage ◽  
Beta Gene ◽  
Receptor Beta

T-cell receptor beta (TCR beta) gene rearrangements occur in a third of early B-cell acute lymphoblastic leukemias (ALLs). V, D, and J segments involved in these inappropriate rearrangements remain unknown and are of interest, both because partial D beta J beta and complete V beta D beta J beta recombinations occur at distinct stages of thymic maturation and because these rearrangements are regulated differently. We have therefore studied in detail seven cases of B-lineage ALL that show inappropriate clonal TCR beta gene rearrangements. Analysis of genomic DNA by Southern hybridization with C beta, J beta 1, V beta 8, and V beta 11 probes suggested the involvement of V beta segment in tumor cell rearrangements. A complete genomic library constructed from one case was screened with a C beta probe, and the TCR beta gene rearrangement was cloned and fully sequenced to show an out of frame V beta 2.2-J beta 2.6 recombination. TCR beta gene rearrangements occurring in other cases were further analyzed by polymerase chain reaction (PCR) using J beta and V beta primers and the resulting specific PCR products were sequenced. Evidence of clonal V beta rearrangements was obtained in all cases. These unexpected findings represent the first definitive demonstration that complete V beta(D beta)J beta rearrangements can occur in B-lineage cells and contrast with the previously reported lack of V beta(D beta)J beta rearrangement in B cells from V beta-J beta-C beta-E mu transgenic mice. In the context of increasing evidence that rearrangements are linked to transcription of unrearranged gene segments, these data prompt a search in B-lineage ALL cells for the presence of germline V beta transcripts whose deregulated expression may be linked to early transforming events.

Increased thymocyte CD4+CD8+ cells and T-cell receptor beta gene rearrangements in thymoma

2003 ◽  
Vol 51 (10) ◽  
pp. 481-487 ◽  
Author(s):  
Motoki Sakuraba ◽  
Toshiko Motoji ◽  
Sumio Nitta ◽  
Hideaki Mizoguchi ◽  
Takamasa Ohnuki
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Gene Rearrangements ◽  
Cd8 Cells ◽  
T Cell Receptor Beta ◽  
Beta Gene ◽  
Receptor Beta

scholarly journals Deletion of the mouse T-cell receptor beta gene enhancer blocks alphabeta T-cell development.

1996 ◽  
Vol 93 (15) ◽  
pp. 7877-7881 ◽  
Author(s):  
G. Bouvier ◽  
F. Watrin ◽  
M. Naspetti ◽  
C. Verthuy ◽  
P. Naquet ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
T Cell Development ◽  
Cell Receptor ◽  
Cell Development ◽  
Gene Enhancer ◽  
T Cell Receptor Beta ◽  
Beta Gene ◽  
Receptor Beta

scholarly journals Similar rearrangements of T-cell receptor beta gene in cell lines and uncultured cells from patients with large granular lymphocyte leukemia

Blood ◽  
1988 ◽  
Vol 72 (2) ◽  
pp. 613-615 ◽  
Author(s):  
TP Jr Loughran ◽  
G Starkebaum ◽  
FW Ruscetti
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Lines ◽  
Interleukin 2 ◽  
Cell Receptor ◽  
Large Granular Lymphocyte ◽  
Phenotypic Analysis ◽  
T Cell Receptor Beta ◽  
Beta Gene ◽  
Receptor Beta

Abstract We established interleukin-2-(IL-2) dependent cell lines from three patients with large granular lymphocyte (LGL) leukemia. Phenotypic analysis demonstrated retention of the CD3+, CD8+ phenotype that was observed in the original leukemic LGL. Unique rearrangements of T-cell receptor beta gene occurring in uncultured leukemic LGL, were also found in cell lines, which suggests that the cell lines were derived from the original leukemic LGL clone in each case.

scholarly journals Interleukin-7: a cofactor for V(D)J rearrangement of the T cell receptor beta gene

Science ◽  
1993 ◽  
Vol 261 (5117) ◽  
pp. 93-95 ◽  
Author(s):  
K Muegge ◽  
M. Vila ◽  
S. Durum
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Interleukin 7 ◽  
T Cell Receptor Beta ◽  
Beta Gene ◽  
Receptor Beta
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