Delayed hypersensitivity reactions in guinea-pigs using cotton pellets; Demonstration of macrophage migration inhibition activity in the cell free exudate

1979 ◽  
Vol 127 (1) ◽  
pp. 1-10 ◽  
Author(s):  
S. Yamamoto ◽  
A. Ishikura
Keyword(s):  
Guinea Pigs ◽  
Delayed Hypersensitivity ◽  
Macrophage Migration ◽  
Hypersensitivity Reactions ◽  
Inhibition Activity ◽  
Migration Inhibition ◽  
Macrophage Migration Inhibition

scholarly journals SEPARABLE POPULATIONS OF ACTIVATED THYMUS-DERIVED LYMPHOCYTES IDENTIFIED IN TWO ASSAYS FOR CELL-MEDIATED IMMUNITY TO MURINE TUMOR ALLOGRAFTS

1974 ◽  
Vol 140 (1) ◽  
pp. 267-289 ◽  
Author(s):  
Robert E. Tigelaar ◽  
R. M. Gorczynski
Keyword(s):  
Cytotoxic Activity ◽  
Velocity Sedimentation ◽  
Target Cells ◽  
Cell Mediated Immunity ◽  
Macrophage Migration ◽  
Inhibition Activity ◽  
Migration Inhibition ◽  
Spleen Cells ◽  
Macrophage Migration Inhibition

The immune response of C57BL mice to a DBA/2 tumor allograft has been assessed in two assays of cell-mediated immunity, the in vitro lysis of 51Cr-labeled target cells and the antigen-mediated inhibition of macrophage migration. Both assays were shown to be measuring a T-cell-mediated reaction. Three types of experiments suggested that distinct subpopulations of T cells mediate these reactions. The tissue distributions of these activities was distinctive; both activities were present in spleens from i.p. immunized mice, but only macrophage migration inhibition activity was found in the peripheral lymph nodes (PLN) of such mice. Adoptive transfer of immune spleen cells into irradiated syngeneic recipients revealed that while a substantial amount of migration inhibition activity could subsequently be found in PLN, cytotoxic activity was found predominantly in the spleens of these adoptive hosts. Velocity sedimentation analysis of immune cells 14 days after i.p. immunization indicated that while the majority of cytotoxic activity was associated with small and medium lymphocytes, the majority of migration inhibition activity was associated with medium and large lymphocytes. In addition, normal spleen cells were fractionated by velocity sedimentation immediately before allosensitization in vitro. Subsequent analysis of the sensitized fractions revealed that the activity profiles for cytotoxicity and macrophage migration inhibition were not coincident. The implications of these observations are discussed.

scholarly journals IN VITRO STUDIES OF THE SUPPRESSION OF DELAYED HYPERSENSITIVITY BY THE INDUCTION OF PARTIAL TOLERANCE

1970 ◽  
Vol 131 (3) ◽  
pp. 603-610 ◽  
Author(s):  
Yves Borel ◽  
John R. David
Keyword(s):  
In Vitro Studies ◽  
Delayed Hypersensitivity ◽  
Macrophage Migration ◽  
Factor Activity ◽  
Migration Inhibition ◽  
Macrophage Migration Inhibition ◽  
Induction Of Tolerance

Suppression of delayed hypersensitivity in vivo is correlated in vitro with the absence of macrophage migration inhibition in the presence of the antigen used to induce partial tolerance. The suppression of delayed hypersensitivity is antigen-specific in vivo as well as in vitro. The lymphocytes, and not the macrophages, are the cells involved in the induction of tolerance in terms of delayed hypersensitivity which is characterized by an absence of migratory factor activity.

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