Comparison of some simple methods of preparing γ-globulin and antiglobulin sera for use in the indirect immunofluorescence technique

1969 ◽  
Vol 98 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Ann Mostratos ◽  
T. S. L. Beswick
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunofluorescence Technique ◽  
Indirect Immunofluorescence Technique

scholarly journals Prevalence of antinuclear autoantibodies in the serum of normal blood dornors

2003 ◽  
Vol 58 (6) ◽  
pp. 315-319 ◽  
Author(s):  
Solange Assuncion Villagra Fernandez ◽  
Alice Zoghbi Coelho Lobo ◽  
Zilda Najjar Prado de Oliveira ◽  
Ligia Maria Ichimura Fukumori ◽  
Alexandre Marques Périgo ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Clinical Condition ◽  
Blood Donors ◽  
Antinuclear Antibodies ◽  
Normal Blood ◽  
Healthy Population ◽  
Age Group ◽  
Immunofluorescence Technique ◽  
Antinuclear Autoantibodies ◽  
Indirect Immunofluorescence Technique

OBJECTIVE:To examine the presence of serum antinuclear autoantibodies in a healthy population. METHODS: Serum of 500 normal blood donors between 18 and 60 years of age were tested for the presence of autoantibodies. Antinuclear antibodies were detected by indirect immunofluorescence technique using HEp-2 epithelial cells as the substrate. The presence of dnaN was detected by indirect immunofluorescence technique using Critidia lucillae as the substrate. Anti-SSA (RO), anti-SSB (LA), anti-Sm, and anti-RNP were determined by double radial immunodiffusion. RESULTS: In the evaluation of the presence of serum antibodies, antinuclear antibodies were detected in 22.6% of the sera. The presence of other antibodies was not significant. The majority of the titers were 1:40. CONCLUSION: The presence of autoantibodies is not necessarily pathologic and has to be related to the age group, gender, and clinical condition of the patient.

scholarly journals Limited reliability of the indirect immunofluorescence technique for the detection of anti-Rib-P antibodies

2008 ◽  
Vol 10 (6) ◽  
pp. R131 ◽  
Author(s):  
Michael Mahler ◽  
Jennifer T Ngo ◽  
Johannes Schulte-Pelkum ◽  
Tanja Luettich ◽  
Marvin J Fritzler
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunofluorescence Technique ◽  
Indirect Immunofluorescence Technique

Development of an indirect immunofluorescence technique for the diagnosis of toxoplasmosis in bottlenose dolphins

2013 ◽  
Vol 113 (2) ◽  
pp. 451-455 ◽  
Author(s):  
María José Bernal-Guadarrama ◽  
Joan Salichs ◽  
Javier Almunia ◽  
Daniel García-Parraga ◽  
Nuhacet Fernández-Gallardo ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Bottlenose Dolphins ◽  
Immunofluorescence Technique ◽  
Indirect Immunofluorescence Technique

scholarly journals Localization of Lipoxygenases 1 and 2 in Germinating Soybean Seeds by an Indirect Immunofluorescence Technique

1983 ◽  
Vol 73 (2) ◽  
pp. 262-267 ◽  
Author(s):  
Marjan Vernooy-Gerritsen ◽  
Adri L. M. Bos ◽  
Gerrit A. Veldink ◽  
Johannes F. G. Vliegenthart
Keyword(s):  
Indirect Immunofluorescence ◽  
Soybean Seeds ◽  
Immunofluorescence Technique ◽  
Indirect Immunofluorescence Technique

Cellular actin and junction formation during reaggregation of adult rat hepatocytes into epithelial cell sheets

1978 ◽  
Vol 31 (1) ◽  
pp. 341-353
Author(s):  
A. Miettinen ◽  
I. Virtanen ◽  
E. Linder
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cell ◽  
Indirect Immunofluorescence ◽  
Rat Hepatocytes ◽  
Immunofluorescence Technique ◽  
Cell Sheets ◽  
Adult Rat ◽  
Junction Formation ◽  
Adult Rat Hepatocytes ◽  
Indirect Immunofluorescence Technique

Aggregation of adult rat hepatocytes, isolated by the collagenase perfusion technique, was studied by ultrastructural methods and the indirect immunofluorescence technique using anti-actin antibodies. In a primary culture the cells rapidly made contact with each other by filopodia-like structures, as seen by scanning electron microscopy. In a few hours this led to stable adhesion of the cells. No identifiable junction formation occurred during the first 17 h in culture. Within 48 h the cells had formed epithelial cell sheets with junctional complexes consisting of tight junctions, bile canaliculus-like structures and zonula adhaerens-type junctions. The distribution of cytoplasmic actin fluorescence remained homogenous in the contacting cells during the first 24 h in culture, as seen with anti-actin antibodies by the indirect immunofluorescence technique. The first short, fluorescent actin filaments appeared in the periphery of the developing lamellipodia of the spreading cell islands. In organized epithelial cell sheets these filaments were seen as long fibres ending at the perinuclear region of the marginal cells. In the submarginal cells fluorescent actin fibres were distinct at the junctional regions of the cells. This filamentous fluorescence seemed to extend throughout the entire cell sheet in an organized manner and correspond to the apical layer of parallel microfilaments seen in transmission electron microscopy. Our results suggest that filamentous actin plays a role in the contact-induced spreading of the cells and in the maintenance of the internal organization of the epithelial cell sheets.

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