Maternal prediabetes and the fœtal pancreas

1957 ◽  
Vol 74 (1) ◽  
pp. 223-226 ◽  
Author(s):  
N. Woolf ◽  
W. P. U. Jackson
Keyword(s):  
Foetal Pancreas

Cultured epithelial cells derived from human foetal pancreas as a model for the study of cystic fibrosis: further analyses on the origins and nature of the cell types

1988 ◽  
Vol 90 (1) ◽  
pp. 73-77
Author(s):  
A. Harris ◽  
L. Coleman
Keyword(s):  
Cystic Fibrosis ◽  
Tissue Culture ◽  
Epithelial Cells ◽  
Culture System ◽  
Cultured Cells ◽  
Cell Types ◽  
Cultured Epithelial Cells ◽  
Different Cell Types ◽  
Foetal Pancreas

The establishment of a tissue-culture system for epithelial cells derived from human foetal pancreas has recently been reported. Further analyses have now been made on these cells in vitro, together with parallel investigation of the distribution of different cell types within the intact foetal pancreas. Results support the view that the cultured cells are ductal in origin and nature. Pancreatic epithelial cell cultures have also been established from foetuses with cystic fibrosis.

The immunology of the human foetal pancreas aged 8–13 gestational weeks

1991 ◽  
Vol 4 (1) ◽  
pp. 195-199 ◽  
Author(s):  
L. C. Koo Seen Lin ◽  
K. I. Welsh ◽  
C. G. Koffman ◽  
I. McColl
Keyword(s):  
Foetal Pancreas

Ontogeny of salivary peptide P-C like immunoreactivity in human pancreatic B-cells

1983 ◽  
Vol 103 (4) ◽  
pp. 552-557 ◽  
Author(s):  
Seiki Ito ◽  
Satoko Isemura ◽  
Eiichi Saitoh ◽  
Kazuo Sanada ◽  
Toshimitsu Suzuki ◽  
...  
Keyword(s):  
B Cells ◽  
Insulin Release ◽  
Protein C ◽  
Calcium Concentration ◽  
Salivary Protein ◽  
Human Pancreas ◽  
Amino Acid Residues ◽  
Salivary Peptide ◽  
Pancreatic B Cells ◽  
Foetal Pancreas

Abstract. An immunohistochemical study using antisera against proline rich salivary peptide P-C and insulin, glucagon, somatostatin and pancreatic polypeptide antisera was carried out on the foetal pancreas at different stages and on the newborn infant's, infant's, child's and adult pancreas to examine the time at which salivary peptide P-C like immunoreactivity appeared in the human pancreas. Salivary peptide P-C like immunoreactive cells first appeared as a few scattered cells in the foetal pancreas after 16 weeks of gestation and gradually increased in numbers during gestation. The cells corresponded only to insulin immunoreactive cells in the foetal, newborn infant's, infant's, child's and adult pancreas. Only some of the insulin immunoreactive cells in the foetal pancreas contained salivary peptide P-C like immunoreactivity while the majority of those in the infant's pancreas and all those in the child's and adult pancreas did so. The findings, together with the fact that the full sequence of salivary peptide P-C is identical to the COOH-terminal 44 amino acid residues of Salivary Protein C, led to the possibility that peptide P-C like immunoreactivity in the human pancreatic B-cells was not a moiety of the precursor of insulin and pro-insulin, but a moiety of Salivary Protein C. It has been suggested that, in saliva, Salivary Protein C aids in maintenance of the calcium concentration. Based on the hypothesis that peptide P-C like immunoreactivity in the human pancreatic B-cells may play some role in insulin release through the maintenance of the calcium concentration, the present finding seems to explain the fact that the mechanism for insulin release in the foetal pancreas is immature in spite of sufficient biosynthesis of insulin.

INSULIN RELEASE FROM HUMAN FOETAL PANCREAS IN TISSUE CULTURE

1973 ◽  
Vol 59 (1) ◽  
pp. 65-79 ◽  
Author(s):  
F. N. LEACH ◽  
M. A. ASHWORTH ◽  
A. J. BARSON ◽  
R. D. G. MILNER
Keyword(s):  
Tissue Culture ◽  
Insulin Release ◽  
Culture Medium ◽  
Calf Serum ◽  
Insulin Degradation ◽  
Optimal Conditions ◽  
Plasma Clot ◽  
Collagenase Digestion ◽  
Incubation Experiments ◽  
Foetal Pancreas

SUMMARY Various methods of tissue culture were studied in an attempt to grow human foetal pancreas under conditions favourable for insulin release. Simple dicing of pancreas was superior to plasma clot adhesion or collagenase digestion for the preparation of tissue for culture. The culture medium described by Kahri (1966) (containing 25% heated postnatal calf serum) was the most suitable of four tested for the study of insulin release from the tissue culture. In this medium insulin could be measured quantitatively by radioimmunoassay and insulin degradation occurred at the rate of 20–25%/24 h. In other media the pancreas grew less well or insulin degradation was much greater. Human foetal pancreas grown under optimal conditions released insulin for up to 34 days. Insulin released into the culture medium did not appear to inhibit the further release of insulin. In some experiments the total amount of insulin released into the culture medium was several-fold greater than that in the pancreas originally seeded. In acute incubation experiments barium and theophylline stimulated insulin release from pancreas cultures. It proved impossible to identify the cells from which insulin was released but they did not appear to be in the monolayer which was composed of fibroblasts.

THE SECRETION OF INSULIN FROM FOETAL AND POSTNATAL RABBIT PANCREAS IN VITRO IN RESPONSE TO VARIOUS SUBSTANCES

1969 ◽  
Vol 44 (2) ◽  
pp. 267-272 ◽  
Author(s):  
R. D. G. MILNER
Keyword(s):  
Insulin Secretion ◽  
Incubation Medium ◽  
Β Cell ◽  
Functional Competence ◽  
Stimulate Insulin Secretion ◽  
Rabbit Pancreas ◽  
Foetal Pancreas

SUMMARY Pieces of pancreas from 24-day and 30-day rabbit foetuses, 1-day-old rabbits and rabbits aged approximately 8 weeks were incubated in vitro and insulin secretion into the incubation medium was measured in response to a variety of stimuli. Glucagon, leucine, ouabain and potassium were effective stimuli at all ages studied. By the criteria of response chosen for these experiments, glucose did not stimulate insulin secretion from 24-day foetal pancreas but did so when pancreas from older animals was studied. It was concluded that the foetal β cell of the rabbit on the 24th day of gestation, although morphologically immature, shows evidence of functional competence.

INSULIN RELEASE FROM HUMAN FOETAL PANCREAS IN RESPONSE TO GLUCOSE, LEUCINE AND ARGININE

1972 ◽  
Vol 52 (3) ◽  
pp. 497-505 ◽  
Author(s):  
R. D. G. MILNER ◽  
M. A. ASHWORTH ◽  
A. J. BARSON
Keyword(s):  
Body Weight ◽  
Insulin Release ◽  
Basal Insulin ◽  
Exocrine Pancreas ◽  
Significant Rise ◽  
Free Medium ◽  
Β Cell ◽  
Foetal Pancreas ◽  
Human Foetuses

SUMMARY Pieces of pancreas from human foetuses of 14–24 weeks gestational age and weighing between 50 and 625 g were incubated in vitro. Insulin release was studied under control conditions and in media supplemented with glucose (3 mg/ml), leucine (5 mmol/1) or arginine (5 mmol/1). Glucose never caused a significant rise in insulin release from the pancreas. The failure of mannoheptulose (3 mg/ml) and 2-deoxyglucose (3 mg/ml) to suppress basal insulin release in a glucose-free medium indicated that basal insulin release was not governed by the liberation of glucose from glycogen in the exocrine pancreas. Arginine stimulated insulin release in four experiments using pancreas from foetuses weighing more than 200 g, but failed to cause insulin release from the pancreas of foetuses weighing less than 200 g in three experiments. Leucine consistently stimulated insulin release from the pancreas of foetuses of less than 200 g body weight but was only variably effective in causing insulin release from pancreas of foetuses weighing more than 200 g. The experimental results illustrate the development of different mechanisms for the release of insulin from the human foetal β cell.

INSULIN CONTENT AND ENZYME HISTOCHEMISTRY OF THE HUMAN FOETAL PANCREATIC ISLET

1966 ◽  
Vol 36 (2) ◽  
pp. 151-NP ◽  
Author(s):  
T. ADESANYA ◽  
I. GRILLO ◽  
K. SHIMA
Keyword(s):  
Pancreatic Islet ◽  
Enzyme Histochemistry ◽  
Islet Cells ◽  
Histochemical Study ◽  
Fluorescent Antibody ◽  
Oxidative Enzymes ◽  
Fluorescent Antibody Technique ◽  
Pancreatic Islet Cells ◽  
Antibody Technique ◽  
Foetal Pancreas

SUMMARY The occurrence of insulin in the developing human pancreatic islet was investigated histochemically and by immunoassay. A parallel histochemical study of enzymes in each foetal pancreas was also made. Oxidative enzymes and the phosphatases, glucose-6-phosphatase and acid phosphatase were localized in pancreatic islet cells from foetuses of 80 mm. crown-rump (CR) length onwards. Insulin was demonstrated both by the fluorescent antibody technique and by immunoassay in the pancreatic extracts of all foetuses of over 80 mm. CR length. No insulin was present in the extract of the pancreas of a 35 mm. foetus. Since β-granulation occurred in foetuses of 90–110 mm. CR length it became obvious that insulin formation starts before β-granulation.

THE EFFECT OF MATERNALLY ADMINISTERED CORTISONE AND ACTH UPON THE PANCREAS OF THE FOETUS

1956 ◽  
Vol 14 (3) ◽  
pp. 284-291 ◽  
Author(s):  
J. LEE ◽  
P. A. RING
Keyword(s):  
Birth Weight ◽  
Treated Group ◽  
Diabetic Mother ◽  
Marked Variation ◽  
Early Maturation ◽  
Foetal Mortality ◽  
Islet Tissue ◽  
Foetal Pancreas ◽  
Maternal Administration

SUMMARY ACTH was administered to pregnant rabbits in doses of 5, 3 and 1·5mg twice daily from the 19th to 26th day of pregnancy. Cortisone 4 mg/kg daily was given to rabbits from the 20th to 27th day of pregnancy. The maternal administration of ACTH (except in the lowest doses) was associated with a high foetal mortality, and few of the liveborn rabbitssurvived. Cortisone produced a similar mortality and a marked depression of birth weight. Sections of the pancreas of foetal rabbits showed early maturation and associated degeneration of the islet tissue. The changes after the administration of cortisone were more marked than those following ACTH. ACTH 0·4 mg twice daily, or cortisone 1 mg daily, was given to rats from the 13th to 20th day of pregnancy. Little effect on foetal mortality or average foetal weight was observed, but there was a marked variation in the weight of rats in the same litter in the cortisone-treated group. Some reduction in the amount of islet tissue at birth was seen, but premature maturation was only seen in one 19-day foetus. An attempt has been made to relate the changes produced in the foetal pancreas by the maternal administration of ACTH and cortisone to those seen in the foetus of the diabetic mother.

scholarly journals Persistence of BK Virus in Human Foetal Pancreas Cells

1986 ◽  
Vol 67 (7) ◽  
pp. 1485-1490 ◽  
Author(s):  
J. van der Noordaa ◽  
A. Van Strien ◽  
C. J. A. Sol
Keyword(s):  
Bk Virus ◽  
Foetal Pancreas
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