On certain properties of antisera prepared against human serum and its various protein fractions: Their use in the detection of sensitisation of human red cells with “incomplete” Rh antibody, and on the nature of this antibody

1947 ◽  
Vol 59 (1-2) ◽  
pp. 105-111 ◽  
Author(s):  
R. R. A. Coombs ◽  
A. E. Mourant
Keyword(s):  
Human Serum ◽  
Red Cells ◽  
Protein Fractions ◽  
Human Red Cells

scholarly journals Lymphocyte-mediated lysis of antibody coated human red cells in the presence of human serum

Blood ◽  
1979 ◽  
Vol 53 (6) ◽  
pp. 1197-1202
Author(s):  
RJ Kurlander ◽  
WF Rosse
Keyword(s):  
Human Serum ◽  
Culture Medium ◽  
Human Albumin ◽  
Peripheral Blood Lymphocytes ◽  
Red Cells ◽  
The Other ◽  
High Concentration ◽  
The Third ◽  
Human Red Cells

When peripheral blood lymphocytes and human red cells coated with IgG were incubated in vitro in culture medium, antibody-dependent lymphocyte-mediated lysis was observed. This lysis was markedly inhibited by the addition of purified monoclonal IgG1 (1000 microgram/ml) to the culture medium. In contrast, lysis by lymphocytes of sensitized red cells in the presence of undiluted human serum was equal to or greater than lysis in medium alone, even in the presence of IgG1 at 1000 microgram/ml, despite the high concentration of IgG in human serum (6000--19,000 microgram/ml). Serum heated to 56 degrees C for 30 min also restored lysis in the presence of IgG1. When serum was separated into three fractions by passage through a Sephadex G-200 column, the third fraction, which contained proteins with a molecular weight of less than 100,000 d (but neither of the other two fractions nor purified human albumin), restored lymphocyte-mediated lysis in the presence of IgG1.

scholarly journals Lymphocyte-mediated lysis of antibody coated human red cells in the presence of human serum

Blood ◽  
1979 ◽  
Vol 53 (6) ◽  
pp. 1197-1202 ◽  
Author(s):  
RJ Kurlander ◽  
WF Rosse
Keyword(s):  
Human Serum ◽  
Culture Medium ◽  
Human Albumin ◽  
Peripheral Blood Lymphocytes ◽  
Red Cells ◽  
The Other ◽  
High Concentration ◽  
The Third ◽  
Human Red Cells

Abstract When peripheral blood lymphocytes and human red cells coated with IgG were incubated in vitro in culture medium, antibody-dependent lymphocyte-mediated lysis was observed. This lysis was markedly inhibited by the addition of purified monoclonal IgG1 (1000 microgram/ml) to the culture medium. In contrast, lysis by lymphocytes of sensitized red cells in the presence of undiluted human serum was equal to or greater than lysis in medium alone, even in the presence of IgG1 at 1000 microgram/ml, despite the high concentration of IgG in human serum (6000--19,000 microgram/ml). Serum heated to 56 degrees C for 30 min also restored lysis in the presence of IgG1. When serum was separated into three fractions by passage through a Sephadex G-200 column, the third fraction, which contained proteins with a molecular weight of less than 100,000 d (but neither of the other two fractions nor purified human albumin), restored lymphocyte-mediated lysis in the presence of IgG1.

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

Metabolic control of the K+ channel of human red cells

1990 ◽  
Vol 116 (1) ◽  
pp. 19-29 ◽  
Author(s):  
Pedro J. Romero ◽  
Carlos E. Ortíz ◽  
Carmelo Melitto
Keyword(s):  
Metabolic Control ◽  
Red Cells ◽  
K Channel ◽  
Human Red Cells

Interactions between sphered human red cells in tube flow: Technique for measuring the strength of antigen-antibody bonds

1982 ◽  
Vol 23 (2) ◽  
pp. 231-238 ◽  
Author(s):  
Harry L. Goldsmith ◽  
Phil Gold ◽  
Joseph Shuster ◽  
Koichi Takamura
Keyword(s):  
Red Cells ◽  
Tube Flow ◽  
Antigen Antibody ◽  
Human Red Cells
Sign in / Sign up

Export Citation Format

Share Document