Incorporation of two‐dimensional nanomaterials into silk fibroin nanofibers for cardiac tissue engineering

2019 ◽  
Vol 31 (2) ◽  
pp. 248-259 ◽  
Author(s):  
Hojjatollah Nazari ◽  
Asieh Heirani‐Tabasi ◽  
Maryam Hajiabbas ◽  
Masoud Khalili ◽  
Mohammadhossein Shahsavari Alavijeh ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Two Dimensional ◽  
Silk Fibroin Nanofibers

Enhancing mechanical and biodegradation properties of polyvinyl alcohol/silk fibroin nanofibers composite patches for Cardiac Tissue Engineering

2019 ◽  
Vol 255 ◽  
pp. 126510 ◽  
Author(s):  
Mostafa M. Sayed ◽  
Hamouda M. Mousa ◽  
M.R. El-Aassar ◽  
Nehal M. El-Deeb ◽  
Nouby M Ghazaly ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Polyvinyl Alcohol ◽  
Silk Fibroin ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Silk Fibroin Nanofibers

Incorporation of SPION‐casein core‐shells into silk‐fibroin nanofibers for cardiac tissue engineering

2019 ◽  
Vol 121 (4) ◽  
pp. 2981-2993 ◽  
Author(s):  
Hojjatollah Nazari ◽  
Asieh Heirani‐Tabasi ◽  
Maryam Hajiabbas ◽  
Milad Salimi Bani ◽  
Mahnaz Nazari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Silk Fibroin Nanofibers

Application potential of three-dimensional silk fibroin scaffold using mesenchymal stem cells for cardiac regeneration

2021 ◽  
pp. 088532822110185
Author(s):  
Yuksel Cetin ◽  
Merve G Sahin ◽  
Fatma N Kok
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Silk Fibroin ◽  
Cardiac Tissue ◽  
Troponin I ◽  
Cardiac Tissue Engineering ◽  
Swelling Ratio ◽  
Differentiation Potential ◽  
Cardiomyogenic Differentiation

Cardiac tissue engineering focusing on biomaterial scaffolds incorporating cells from different sources has been explored to regenerate or repair damaged area as a lifesaving approach.The aim of this study was to evaluate the cardiomyocyte differentiation potential of human adipose mesenchymal stem cells (hAD-MSCs) as an alternative cell source on silk fibroin (SF) scaffolds for cardiac tissue engineering. The change in surface morphology of SF scaffolds depending on SF concentration (1–6%, w/v) and increase in their porosity upon application of unidirectional freezing were visualized by scanning electron microscopy (SEM). Swelling ratio was found to increase 2.4 fold when SF amount was decreased from 4% to 2%. To avoid excessive swelling, 4% SF scaffold with swelling ratio of 10% (w/w) was chosen for further studies.Biodegradation rate of SF scaffolds depended on enzymatic activity was found to be 75% weight loss of SF scaffolds at the day 14. The phenotype of hAD-MSCs and their multi-linage potential into chondrocytes, osteocytes, and adipocytes were shown by flow cytometry and immunohistochemical staining, respectively.The viability of hAD-MSCs on 3D SF scaffolds was determined as 90%, 118%, and 138% after 1, 7, and 14 days, respectively. The use of 3D SF scaffolds was associated with increased production of cardiomyogenic biomarkers: α-actinin, troponin I, connexin 43, and myosin heavy chain. The fabricated 3D SF scaffolds were proved to sustain hAD-MSCs proliferation and cardiomyogenic differentiation therefore, hAD-MSCs on 3D SF scaffolds may useful tool to regenerate or repair damaged area using cardiac tissue engineering techniques.

Conductive polypyrrole-encapsulated silk fibroin fibers for cardiac tissue engineering

Biomaterials ◽  
2021 ◽  
pp. 121008
Author(s):  
Yeshi Liang ◽  
Aleksandr Mitriashkin ◽  
Ting Ting Lim ◽  
James Cho-Hong Goh
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Conductive Polypyrrole

scholarly journals Conductive silk–polypyrrole composite scaffolds with bioinspired nanotopographic cues for cardiac tissue engineering

2018 ◽  
Vol 6 (44) ◽  
pp. 7185-7196 ◽  
Author(s):  
Jonathan H. Tsui ◽  
Nicholas A. Ostrovsky-Snider ◽  
David M. P. Yama ◽  
Jordan D. Donohue ◽  
Jong Seob Choi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Silk Fibroin ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Human Stem Cell ◽  
Composite Scaffolds ◽  
Polypyrrole Composite

Imparting electroconductive and nanotopographical cues to biodegradable silk–fibroin films enhanced the maturation of cultured human stem cell-derived cardiomyocytes.

scholarly journals Extrinsically Conductive Nanomaterials for Cardiac Tissue Engineering Applications

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 914
Author(s):  
Arsalan Ul Haq ◽  
Felicia Carotenuto ◽  
Paolo Di Nardo ◽  
Roberto Francini ◽  
Paolo Prosposito ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Scar Tissue ◽  
Cardiac Tissue Engineering ◽  
Assistive Device ◽  
Long Run ◽  
Fibrotic Scar ◽  
Regeneration Capability ◽  
Conductive Nanomaterials

Myocardial infarction (MI) is the consequence of coronary artery thrombosis resulting in ischemia and necrosis of the myocardium. As a result, billions of contractile cardiomyocytes are lost with poor innate regeneration capability. This degenerated tissue is replaced by collagen-rich fibrotic scar tissue as the usual body response to quickly repair the injury. The non-conductive nature of this tissue results in arrhythmias and asynchronous beating leading to total heart failure in the long run due to ventricular remodelling. Traditional pharmacological and assistive device approaches have failed to meet the utmost need for tissue regeneration to repair MI injuries. Engineered heart tissues (EHTs) seem promising alternatives, but their non-conductive nature could not resolve problems such as arrhythmias and asynchronous beating for long term in-vivo applications. The ability of nanotechnology to mimic the nano-bioarchitecture of the extracellular matrix and the potential of cardiac tissue engineering to engineer heart-like tissues makes it a unique combination to develop conductive constructs. Biomaterials blended with conductive nanomaterials could yield conductive constructs (referred to as extrinsically conductive). These cell-laden conductive constructs can alleviate cardiac functions when implanted in-vivo. A succinct review of the most promising applications of nanomaterials in cardiac tissue engineering to repair MI injuries is presented with a focus on extrinsically conductive nanomaterials.

scholarly journals Designing of spider silk proteins for hiPSC-based cardiac tissue engineering

2021 ◽  
pp. 100114
Author(s):  
Tilman U. Esser ◽  
Vanessa T. Trossmann ◽  
Sarah Lentz ◽  
Felix B. Engel ◽  
Thomas Scheibel
Keyword(s):  
Tissue Engineering ◽  
Spider Silk ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Silk Proteins

scholarly journals Cardiac Organoids to Model and Heal Heart Failure and Cardiomyopathies

Biomedicines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 563
Author(s):  
Magali Seguret ◽  
Eva Vermersch ◽  
Charlène Jouve ◽  
Jean-Sébastien Hulot
Keyword(s):  
Tissue Engineering ◽  
Drug Testing ◽  
Cardiac Tissue ◽  
Cardiac Tissue Engineering ◽  
Cardiac Cells ◽  
Cardiac Functions ◽  
Different Types ◽  
Recent Developments ◽  
Human Cardiac Tissue ◽  
Key Aspects

Cardiac tissue engineering aims at creating contractile structures that can optimally reproduce the features of human cardiac tissue. These constructs are becoming valuable tools to model some of the cardiac functions, to set preclinical platforms for drug testing, or to alternatively be used as therapies for cardiac repair approaches. Most of the recent developments in cardiac tissue engineering have been made possible by important advances regarding the efficient generation of cardiac cells from pluripotent stem cells and the use of novel biomaterials and microfabrication methods. Different combinations of cells, biomaterials, scaffolds, and geometries are however possible, which results in different types of structures with gradual complexities and abilities to mimic the native cardiac tissue. Here, we intend to cover key aspects of tissue engineering applied to cardiology and the consequent development of cardiac organoids. This review presents various facets of the construction of human cardiac 3D constructs, from the choice of the components to their patterning, the final geometry of generated tissues, and the subsequent readouts and applications to model and treat cardiac diseases.

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