scholarly journals Cell enrichment and separation technique using active hydrogel membranes

PAMM ◽  
2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Ramona Mehling ◽  
Denise Gruner ◽  
Adrian Ehrenhofer ◽  
Andreas Richter ◽  
Thomas Wallmersperger
Keyword(s):  
Separation Technique ◽  
Hydrogel Membranes ◽  
Cell Enrichment

scholarly journals PCGF1 promotes epigenetic activation of stemness markers and colorectal cancer stem cell enrichment

2021 ◽  
Vol 12 (7) ◽  
Author(s):  
Guangyu Ji ◽  
Wenjuan Zhou ◽  
Jingyi Du ◽  
Juan Zhou ◽  
Dong Wu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Stem Cell ◽  
Cancer Progression ◽  
Molecular Mechanisms ◽  
Tumour Progression ◽  
Stem Cell Markers ◽  
Epigenetic Regulator ◽  
Cell Enrichment ◽  
Histone Trimethylation

AbstractColorectal cancer (CRC) stem cells are resistant to cancer therapy and are therefore responsible for tumour progression after conventional therapy fails. However, the molecular mechanisms underlying the maintenance of stemness are poorly understood. In this study, we identified PCGF1 as a crucial epigenetic regulator that sustains the stem cell-like phenotype of CRC. PCGF1 expression was increased in CRC and was significantly correlated with cancer progression and poor prognosis in CRC patients. PCGF1 knockdown inhibited CRC stem cell proliferation and CRC stem cell enrichment. Importantly, PCGF1 silencing impaired tumour growth in vivo. Mechanistically, PCGF1 bound to the promoters of CRC stem cell markers and activated their transcription by increasing the H3K4 histone trimethylation (H3K4me3) marks and decreasing the H3K27 histone trimethylation (H3K27me3) marks on their promoters by increasing expression of the H3K4me3 methyltransferase KMT2A and the H3K27me3 demethylase KDM6A. Our findings suggest that PCGF1 is a potential therapeutic target for CRC treatment.

scholarly journals A New Radiocarbon Pretreatment Method for Molluscan Shell Using Density Fractionation of Carbonates in Bromoform

Radiocarbon ◽  
2010 ◽  
Vol 52 (3) ◽  
pp. 1301-1311 ◽  
Author(s):  
Christopher M Russo ◽  
Jennifer A Tripp ◽  
Katerina Douka ◽  
Thomas F G Higham
Keyword(s):  
Radiocarbon Dating ◽  
Separation Technique ◽  
Archaeological Sites ◽  
Density Fractionation ◽  
Pretreatment Method ◽  
Density Separation ◽  
Molluscan Shell ◽  
Archaeological Artifacts ◽  
Organic Remains

Coastal archaeological sites that lack organic remains for radiocarbon dating are often abundant sources of molluscan shells. As a substitute for materials such as bone and charcoal, shells can be analyzed with 14C dating to determine a site's age. Despite their being convenient, non-mobile archaeological artifacts, molluscan shells are plagued by several issues, including carbonate remodeling, in which aragonite in shells is converted to calcite as predicted by thermodynamics. We present here a carbonate density separation technique that addresses the issue of carbonate remodeling. Using a density fractionation with bromoform, aragonite concentrations are enriched in shells that have undergone significant remodeling. The technique has been applied to archaeological shells and has returned dates that are younger than those previously determined for the same shells.

scholarly journals Verification of Outstanding Claim Provisions—Separation Technique

1977 ◽  
Vol 9 (1-2) ◽  
pp. 26-32 ◽  
Author(s):  
R. E. Beard
Keyword(s):  
Distribution Function ◽  
Automatic Verification ◽  
Separation Technique ◽  
Implicit Assumption ◽  
Subjective Judgment ◽  
Constant Rate ◽  
The Future ◽  
Average Distribution ◽  
Chain Ladder ◽  
A Chain

In reference [1] Dr. G. C. Taylor has described a useful advance in the techniques available for verification of outstanding claims estimates when the data provided is the cohort development of numbers and amounts of claims. In this note it is assumed that the numbers relate to settled claims and that the amounts relate to claim payments, so there is an implicit assumption that the pattern of partial payments is constant. If the amounts of settled claims were to be used, there would be a one/one relationship between the numbers and amounts, but the effect of the exogeneous factor would be blurred because the settlements in a year other than the first include partial payments made some time previously, and, by hypothesis, based on different factors. If information relating to partial payments is available the data can be examined for any major fluctuation in the pattern and allowance made accordingly.In paragraph (2) of reference [1] a brief description is given of a standard routine calculation in which the average distribution function of claim payments in time is estimated from the triangle of payments by a chain ladder technique. This distribution function is then used to estimate the expected development of the incomplete cohorts, the implicit assumption being made that the function was stable in time. With a constant rate of inflation the results obtained by this technique were found to be satisfactory but with a rapid increase in the rate of inflation the distribution function changed so that projection led to underestimates of the future claims payments. Various methods of adjusting the projections to allow for the change in the rate of inflation have been investigated, but they all involve an important element of subjective judgment and so far no generally suitable basis for “automatic” verification by this particular technique has been discovered. See however reference [2].

scholarly journals Cancer stem cell enrichment is associated with enhancement of nicotinamide N‐methyltransferase expression

IUBMB Life ◽  
2020 ◽  
Vol 72 (7) ◽  
pp. 1415-1425 ◽  
Author(s):  
Valentina Pozzi ◽  
Eleonora Salvolini ◽  
Guendalina Lucarini ◽  
Alessia Salvucci ◽  
Roberto Campagna ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Cell Enrichment

Human breast cancer cell enrichment by Dean flow driven microfluidic channels

2015 ◽  
Vol 22 (3) ◽  
pp. 645-652 ◽  
Author(s):  
M. Zuvin ◽  
N. Mansur ◽  
S. Z. Birol ◽  
L. Trabzon ◽  
A. Sayı Yazgan
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Human Breast Cancer ◽  
Human Breast Cancer Cell ◽  
Microfluidic Channels ◽  
Human Breast ◽  
Dean Flow ◽  
Cell Enrichment

A novel separation technique - gas carrying evaporation: theory and application

2003 ◽  
Vol 78 (2-3) ◽  
pp. 173-180 ◽  
Author(s):  
Feng Ma ◽  
Zhijia Yu ◽  
Jun Wang ◽  
Jie Ding ◽  
Weiqin Xu ◽  
...  
Keyword(s):  
Separation Technique
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