scholarly journals Characterization of Highly Efficient RNA-Cleaving DNAzymes that Function at Acidic pH with No Divalent Metal-Ion Cofactors

ChemistryOpen ◽  
2016 ◽  
Vol 6 (1) ◽  
pp. 46-56 ◽  
Author(s):  
Aleksandra Kasprowicz ◽  
Kamila Stokowa-Sołtys ◽  
Małgorzata Jeżowska-Bojczuk ◽  
Jan Wrzesiński ◽  
Jerzy Ciesiołka
Keyword(s):  
Metal Ion ◽  
Divalent Metal ◽  
Acidic Ph ◽  
Highly Efficient ◽  
Divalent Metal Ion ◽  
Metal Ion Cofactors

Characterization of α-Farnesene Synthase from `Delicious' Apples

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 532d-532
Author(s):  
H.P.V. Rupasinghe ◽  
G. Paliyath ◽  
D.P. Murr
Keyword(s):  
Enzyme Activity ◽  
Metal Ion ◽  
Divalent Metal ◽  
Farnesyl Pyrophosphate ◽  
Anaerobic Conditions ◽  
Ph Optimum ◽  
Divalent Metal Ion ◽  
Apple Cultivars ◽  
Inherent Nature

α-Farnesene metabolism is associated with the occurrence of superficial scald in pome fruits. Trans,trans-α-farnesene synthase, which catalyzes the terminal step of α-farnesene biosynthesis viz. conversion of farnesyl pyrophosphate to α-farnesene, has been characterized in the extract from skin tissues of `Delicious' apples (Malus domestica Borkh.). The total and specific activities of the enzyme were the highest in the cytosolic fraction when compared to that in membrane fractions. The enzyme possessed a pH optimum of 5.6 and required a divalent metal ion (Mg+2 and Mn+2 were preferred). The activity was highest between 10 and 20 °C, although 50 % of the activity was still retained at 0 °C. The presence of thiol reagents, pyridine dinucleotide effectors, anaerobic conditions or antioxidants did not significantly affect enzyme activity. α-Farnesene synthase activity was similar in the extract of skin tissue from scald-developing and non-scald-developing apples. The enzyme activity was not correlated to the inherent nature of scald-susceptibility or resistance in eight different apple cultivars tested.

New divalent metal ion complexes with 1,8-diaminonapthalene -2-thione: Synthesis, Spectroscopic, anti-bacterial and anticancer activity studies

2021 ◽  
pp. 131291
Author(s):  
Ahmed S. Faihan ◽  
Mohammad R. Hatshan ◽  
Ali S. Alqahtani ◽  
Fahd A. Nasr ◽  
Subhi A. Al-Jibori ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Metal Ion ◽  
Divalent Metal ◽  
Metal Ion Complexes ◽  
Divalent Metal Ion

Divalent metal ion-sensitive holographic sensors

2005 ◽  
Vol 528 (2) ◽  
pp. 219-228 ◽  
Author(s):  
Blanca Madrigal González ◽  
Graham Christie ◽  
Colin A.B. Davidson ◽  
Jeff Blyth ◽  
Christopher R. Lowe
Keyword(s):  
Metal Ion ◽  
Divalent Metal ◽  
Divalent Metal Ion
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