scholarly journals Collection and storage of human blood and adipose for genomic analysis of clinical samples

2011 ◽  
Vol 34 (5) ◽  
pp. 408-418 ◽  
Author(s):  
Ann K. Cashion ◽  
Reba A. Umberger ◽  
Shirlean B. Goodwin ◽  
Thomas R. Sutter
Keyword(s):  
Human Blood ◽  
Genomic Analysis ◽  
Clinical Samples ◽  
And Storage

The Sampling and Storage of Arterial Human Blood

2015 ◽  
pp. 14-19
Author(s):  
O. M�ller-Plathe ◽  
H. Schlebusch
Keyword(s):  
Human Blood ◽  
And Storage

scholarly journals A LAMP-SNP Assay Detecting C580Y Mutation in Pfkelch13 Gene from Clinically Dried Blood Spot Samples

2021 ◽  
Vol 59 (1) ◽  
pp. 15-22
Author(s):  
Thunchanok Khammanee ◽  
Nongyao Sawangjaroen ◽  
Hansuk Buncherd ◽  
Aung Win Tun ◽  
Supinya Thanapongpichat
Keyword(s):  
Field Study ◽  
Artemisinin Resistance ◽  
Plasmodium Species ◽  
Dried Blood Spot ◽  
Clinical Samples ◽  
Simple Method ◽  
Infected Blood ◽  
And Storage ◽  
C580y Mutation ◽  
Blood Spot

Artemisinin resistance (ART) has been confirmed in Greater Mekong Sub-region countries. Currently, C580Y mutation on Pfkelch13 gene is known as the molecular marker for the detection of ART. Rapid and accurate detection of ART in field study is essential to guide malaria containment and elimination interventions. A simple method for collection of malaria-infected blood is to spot the blood on filter paper and is fast and easy for transportation and storage in the field study. This study aims to evaluate LAMP-SNP assay for C580Y mutation detection by introducing an extra mismatched nucleotide at the 3’ end of the FIP primer. The LAMP-SNP assay was performed in a water bath held at a temperature of 56°C for 45 min. LAMP-SNP products were interpreted by both gel-electrophoresis and HNB-visualized changes in color. The method was then tested with 120 P. falciparum DNA from dried blood spot samples. In comparing the LAMP-SNP assay results with those from DNA sequencing of the clinical samples, the 2 results fully agreed to detect C580Y. The sensitivity and specificity of the LAMP-SNP assay showed 100%. There were no cross-reactions with other Plasmodium species and other Pfkelch13 mutations. The LAMP-SNP assay performed in this study was rapid, reliable, and useful in detecting artemisinin resistance in the field study.

scholarly journals Genomic investigation of clinically significant coagulase-negative staphylococci

2021 ◽  
Author(s):  
Kevin Cole ◽  
Bridget Atkins ◽  
Martin Llewelyn ◽  
John Paul
Keyword(s):  
High Resolution ◽  
Genomic Analysis ◽  
Staphylococcal Infection ◽  
Clinical Samples ◽  
Future Research ◽  
Emerging Pathogens ◽  
Coagulase Negative Staphylococci ◽  
Content Type ◽  
History Of ◽  
Clinically Significant

Introduction. Coagulase-negative staphylococci have been recognized both as emerging pathogens and contaminants of clinical samples. High-resolution genomic investigation may provide insights into their clinical significance. Aims. To review the literature regarding coagulase-negative staphylococcal infection and the utility of genomic methods to aid diagnosis and management, and to identify promising areas for future research. Methodology. We searched Google Scholar with the terms ( Staphylococcus ) AND (sequencing OR (infection)). We prioritized papers that addressed coagulase-negative staphylococci, genomic analysis, or infection. Results. A number of studies have investigated specimen-related, phenotypic and genetic factors associated with colonization, infection and virulence, but diagnosis remains problematic. Conclusion. Genomic investigation provides insights into the genetic diversity and natural history of colonization and infection. Such information allows the development of new methodologies to identify and compare relatedness and predict antimicrobial resistance. Future clinical studies that employ suitable sampling frames coupled with the application of high-resolution whole-genome sequencing may aid the development of more discriminatory diagnostic approaches to coagulase-staphylococcal infection.

scholarly journals Clinical characterization and Genomic analysis of COVID-19 breakthrough infections during second wave in different states of India

2021 ◽  
Author(s):  
Nivedita Gupta ◽  
Harmanmeet Kaur ◽  
Pragya Yadav ◽  
Labanya Mukhopadhyay ◽  
Rima R Sahay ◽  
...  
Keyword(s):  
Hospital Admission ◽  
Clinical Data ◽  
Throat Swab ◽  
Genomic Analysis ◽  
Clinical Samples ◽  
Post Vaccination ◽  
Genome Coverage ◽  
Nasal Swabs ◽  
Second Wave ◽  
Lineage Iv

During March to June 2021 India has experienced a deadly second wave of COVID19 with an increased number of post vaccination breakthrough infections reported across the country. To understand the possible reason of these breakthroughs we collected 677 clinical samples (throat swab/ nasal swabs) of individuals who had received two doses (n=592) and one dose (n=85) of vaccines (Covishield and Covaxin,) and tested positive for COVID19, from 17 states/Union Territories of country. These cases were telephonically interviewed and clinical data was analyzed. A total of 511 SARS-CoV-2 genomes were recovered with genome coverage of higher than 98% from both the cases. Analysis of both the cases determined that 86.69% (n=443) of them belonged to the Delta variant along with Alpha, Kappa, Delta AY.1 and Delta AY.2. The Delta variant clustered into 4 distinct sub-lineages. Sub-lineage I had mutations: ORF1ab, A1306S, P2046L, P2287S, V2930L, T3255I, T3446A, G5063S, P5401L, A6319V and N G215C; Sub lineage II : ORF1ab P309, A3209V, V3718A, G5063S, P5401L and ORF7a L116F; Sub lineage III : ORF1ab A3209V, V3718A, T3750I, G5063S, P5401L and Spike A222V; Sub-lineage IV ORF1ab P309L, D2980N, F3138S and spike K77T. This study indicated that majority of the clinical cases in the breakthrough were infected with the Delta variant and only 9.8% cases required hospitalization while fatality was observed in only 0.4% cases. This clearly suggests that the vaccination does provide reduction in hospital admission and mortality.

scholarly journals Coenzyme Q10 in human blood: Native levels and determinants of oxidation during processing and storage

2010 ◽  
Vol 48 (12) ◽  
pp. 1610-1617 ◽  
Author(s):  
Adrian A. Franke ◽  
Cynthia M. Morrison ◽  
Jesse L. Bakke ◽  
Laurie J. Custer ◽  
Xingnan Li ◽  
...  
Keyword(s):  
Human Blood ◽  
Coenzyme Q10 ◽  
Processing And Storage ◽  
And Storage

scholarly journals Collection and Storage of Human Blood Cells for mRNA Expression Profiling: A 15-Month Stability Study

2005 ◽  
Vol 51 (7) ◽  
pp. 1250-1252 ◽  
Author(s):  
Jean-Brice Marteau ◽  
Steve Mohr ◽  
Michèle Pfister ◽  
Sophie Visvikis-Siest
Keyword(s):  
Mrna Expression ◽  
Human Blood ◽  
Expression Profiling ◽  
Blood Cells ◽  
Stability Study ◽  
Human Blood Cells ◽  
Mrna Expression Profiling ◽  
And Storage

The stability of 25-hydroxyvitamin D in human blood during different sampling and storage conditions

Bone ◽  
2009 ◽  
Vol 44 ◽  
pp. S364
Author(s):  
S. við Streym Thomsen⁎ ◽  
L. Rejnmark ◽  
P. Vestergaard ◽  
L. Heickendorff ◽  
L. Mosekilde
Keyword(s):  
Human Blood ◽  
Storage Conditions ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
The Stability ◽  
And Storage

Antifungal susceptibility of non-albicans Candida spp. isolated from raw milk and human blood in Alborz and Tehran provinces

2020 ◽  
Author(s):  
Zahra Namvar ◽  
Abbas Akhavan Sepahy ◽  
Robab Rafiei Tabatabaei ◽  
Sassan Rezaie
Keyword(s):  
Human Blood ◽  
Fungal Infections ◽  
Antifungal Susceptibility ◽  
Raw Milk ◽  
Term Treatment ◽  
Clinical Samples ◽  
Candida Spp ◽  
Long Term Treatment ◽  
High Prevalence ◽  
Quality Of Milk

Background and Objectives: Recent reports indicate high prevalence of fungal infections due to non-albicans Candida spp. which are present in various environments such as raw milk. The quality of milk for fungal normal flora was investigated in this study. Materials and Methods: A total of 262 milk samples were collected directly from milk collection tanks indesignated dairy farms and cultured in SDA media. By further analysis of grown yeasts, 69 non-albicans Candida strains were identified. Antifungal susceptibility of the isolated species, were evaluated against amphotericin B, itraconazole, fluconazole and flucytosine. Fifty two non-albicans clinical samples isolated from human blood have been evaluated along. Results: Antifungal susceptibility evaluation in non-albicans strains isolated from milk revealed Candida glabrata and Candida tropicalis to be 100% sensitive to flucytosine and fluconazole. Candida krusei showed 94% and 80% sensitivity to flucytosine and fluconazole respectively. Candida parapsilosis indicated 72.72% sensitivity to fluconazole. Conclusion: Evaluation of non-albicans Candida species in raw milk and antifungal susceptibility patterns of these isolatescompare with non-albicansisolates from human blood, may help physicians to choose an appropriate medication for diseases needing long-term treatment, especially for diseases caused by local strains.

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