Directional control of neurite outgrowth from cultured hippocampal neurons is modulated by the lectin concanavalin A

1992 ◽  
Vol 23 (4) ◽  
pp. 354-363 ◽  
Author(s):  
Lynne M. Farmer ◽  
J�rg Hagmann ◽  
Daniel Dagan ◽  
Andrew I. Matus ◽  
Irwin B. Levitan
Keyword(s):  
Neurite Outgrowth ◽  
Concanavalin A ◽  
Hippocampal Neurons ◽  
Directional Control ◽  
Cultured Hippocampal Neurons

scholarly journals Differential neurite outgrowth is required for axon specification by cultured hippocampal neurons

2012 ◽  
Vol 123 (6) ◽  
pp. 904-910 ◽  
Author(s):  
Hideaki Yamamoto ◽  
Takanori Demura ◽  
Mayu Morita ◽  
Gary A. Banker ◽  
Takashi Tanii ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Hippocampal Neurons ◽  
Axon Specification ◽  
Cultured Hippocampal Neurons

Serotonin (5-HT) regulates neurite outgrowth through 5-HT1Aand 5-HT7receptors in cultured hippocampal neurons

2014 ◽  
Vol 92 (8) ◽  
pp. 1000-1009 ◽  
Author(s):  
Paulina S. Rojas ◽  
David Neira ◽  
Mauricio Muñoz ◽  
Sergio Lavandero ◽  
Jenny L. Fiedler
Keyword(s):  
Neurite Outgrowth ◽  
Hippocampal Neurons ◽  
Cultured Hippocampal Neurons

scholarly journals Suppression of kinesin expression in cultured hippocampal neurons using antisense oligonucleotides.

1992 ◽  
Vol 117 (3) ◽  
pp. 595-606 ◽  
Author(s):  
A Ferreira ◽  
J Niclas ◽  
R D Vale ◽  
G Banker ◽  
K S Kosik
Keyword(s):  
Neurite Outgrowth ◽  
Cell Body ◽  
Heavy Chain ◽  
Antisense Oligonucleotides ◽  
Hippocampal Neurons ◽  
Full Recovery ◽  
Synapsin I ◽  
Neurite Length ◽  
Protein Levels ◽  
Cultured Hippocampal Neurons

Kinesin, a microtubule-based force-generating molecule, is thought to translocate organelles along microtubules. To examine the function of kinesin in neurons, we sought to suppress kinesin heavy chain (KHC) expression in cultured hippocampal neurons using antisense oligonucleotides and study the phenotype of these KHC "null" cells. Two different antisense oligonucleotides complementary to the KHC sequence reduced the protein levels of the heavy chain by greater than 95% within 24 h after application and produced identical phenotypes. After inhibition of KHC expression for 24 or 48 h, neurons extended an array of neurites often with one neurite longer than the others; however, the length of all these neurites was significantly reduced. Inhibition of KHC expression also altered the distribution of GAP-43 and synapsin I, two proteins thought to be transported in association with membranous organelles. These proteins, which are normally localized at the tips of growing neurites, were confined to the cell body in antisense-treated cells. Treatment of the cells with the corresponding sense oligonucleotides affected neither the distribution of GAP-43 and synapsin I, nor the length of neurites. A full recovery of neurite length occurred after removal of the antisense oligonucleotides from the medium. These data indicate that KHC plays a role in the anterograde translocation of vesicles containing GAP-43 and synapsin I. A deficiency in vesicle delivery may also explain the inhibition of neurite outgrowth. Despite the inhibition of KHC and the failure of GAP-43 and synapsin I to move out of the cell body, hippocampal neurons can extend processes and acquire as asymmetric morphology.

scholarly journals Amphiphysin I Antisense Oligonucleotides Inhibit Neurite Outgrowth in Cultured Hippocampal Neurons

1998 ◽  
Vol 18 (1) ◽  
pp. 93-103 ◽  
Author(s):  
Olaf Mundigl ◽  
Gian-Carlo Ochoa ◽  
Carol David ◽  
Vladimir I. Slepnev ◽  
Alexander Kabanov ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Antisense Oligonucleotides ◽  
Hippocampal Neurons ◽  
Cultured Hippocampal Neurons

scholarly journals Dexmedetomidine alleviates LPS-induced neuronal dysfunction via AKT/GSK-3β/CRMP-2 pathway

2020 ◽  
Author(s):  
Wei Zeng ◽  
Chunyuan Zhang ◽  
Qingshan Long ◽  
Yalan Li
Keyword(s):  
Patch Clamp ◽  
Synaptic Transmission ◽  
Neurite Outgrowth ◽  
Signaling Pathway ◽  
Hippocampal Neurons ◽  
Hippocampal Neuron ◽  
Time Dependent ◽  
Dependent Manner ◽  
Gsk 3Β ◽  
Cultured Hippocampal Neurons

Abstract Background Dexmedetomidine (Dex), an α2-adrenergic receptor agonist, shows intervention effect on cognitive dysfunction in elderly patients after general anesthesia. However, the underlying pathological mechanism remains to be further elucidated. The aim of the study was to investigate effects of Dex on LPS-induced neuronal damages in cultured hippocampal neurons. Methods We applied LPS to mimic the post-surgical inflammation in cultured hippocampal neuron. Cultured hippocampal neurons were treated with LPS in a dose- and time- dependent manner, then were administrated with or without Dex. The neuronal morphology including neurite outgrowth and synaptic transmission was observed, and mEPSCs was recorded by electrophysiological patch clamp. Cell lysates were subjected for western blot to assess the explore the potential underlying mechanism. Results Compared to control group, administration of LPS significantly impaired the neurite outgrowth in a concentration- and time-dependent manner. Dex treatment markedly reversed LPS-induced impairment of neurite outgrowth in hippocampal neurons. Electrophysiological patch clamp results showed that LPS induced synaptic transmission dysfunction, which could be restored after Dex addition. Furthermore, western blotting assays showed that LPS suppressed the AKT/GSK-3β/CRMP-2 signaling pathway and Dex administration significantly re-activated this pathway to encounter the inhibitory effect of LPS. Conclusion Addition of Dex showed significant protection effect on LPS-induced hippocampal neuron damages, including neurite outgrowth and synaptic transmission. Dex functioned via the activation of GSK-3β/CRMP-2 signaling pathway to alleviate LPS-induced neurological dysfunctions.

scholarly journals Ginsenoside Rg1 protects against neurodegeneration by inducing neurite outgrowth in cultured hippocampal neurons

2016 ◽  
Vol 11 (2) ◽  
pp. 319 ◽  
Author(s):  
Qiong-lan Yuan ◽  
Liang Huang ◽  
Li-feng Liu ◽  
Juan Liu ◽  
Ling Dou ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Hippocampal Neurons ◽  
Ginsenoside Rg1 ◽  
Cultured Hippocampal Neurons
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