scholarly journals Unique neuronal tracers show migration and differentiation of SVZ progenitors in organotypic slices

2001 ◽  
Vol 49 (4) ◽  
pp. 326-338 ◽  
Author(s):  
S. De Marchis ◽  
A. Fasolo ◽  
M. Shipley ◽  
A. Puche
Keyword(s):  
Neuronal Tracers

Segmental repetition of neuronal phenotype sets in the chick embryo hindbrain

Development ◽  
1993 ◽  
Vol 118 (1) ◽  
pp. 151-162 ◽  
Author(s):  
J.D. Clarke ◽  
A. Lumsden
Keyword(s):  
Chick Embryo ◽  
Cell Types ◽  
Spatial Segregation ◽  
Differential Migration ◽  
Neuronal Phenotype ◽  
Regional Diversity ◽  
Early Chick Embryo ◽  
Data Support ◽  
Neuronal Types ◽  
Neuronal Tracers

The neurons within the segmented hindbrain of the early chick embryo have been mapped with the neuronal tracers HRP and fluorescent lysinated dextran. We have categorised neurons according to their axonal pathways and have then compared rhombomeres with respect to the number and class of neurons present. The results indicate that most rhombomeres are similar in that they contain the same set of basic neuronal types but differ in that particular neuronal types are more abundant in some rhombomeres than others. The data support the concept that the hindbrain develops according to ‘variations on a segmental theme’ rather than ‘each segment is unique’. Many of the cell types occupy distinct mediolateral domains that are probably established by both the differential migration of some neuronal classes and the spatial segregation of distinct precursors. The caudal rhombomeres 7 and 8 are exceptional in that they do not have the full set of basic neuronal types and also contain two additional medial cell types that are not present rostrally. The mechanisms that may generate the regional diversity apparent in the more mature hindbrain are discussed.

scholarly journals Carbocyanine Postmortem Neuronal Tracing: Influence of Different Parameters on Tracing Distance and Combination with Immunocytochemistry

1998 ◽  
Vol 46 (8) ◽  
pp. 901-910 ◽  
Author(s):  
Julius-Robert Lukas ◽  
Martin Aigner ◽  
Michaela Denk ◽  
Harald Heinzl ◽  
Martin Burian ◽  
...  
Keyword(s):  
Cervical Spinal Cord ◽  
Trigeminal Ganglia ◽  
Detailed Data ◽  
Animal Tissues ◽  
Neuronal Tracing ◽  
Calcitonin Gene ◽  
Sciatic Nerves ◽  
Neuronal Tracers ◽  
Incubation Temperatures ◽  
Newborn Animals

SUMMARY Carbocyanines (DiI, DiA, DiO) are able to travel along membranes by diffusion and therefore have been used as postmortem neuronal tracers in aldehyde-fixed tissues. Surprisingly, detailed data on the influence of different parameters on tracing distances are still missing. This study was carried out to optimize tracing procedures and to reveal the validity of the combination of postmortem tracing with immunocytochemistry. Carbocyanine crystals were applied to the cervical spinal cord, sciatic nerves, and brachial plexuses of humans and guinea pigs. Incubation in the dark at 37C for 12-15 weeks proved optimal to achieve longest tracing distances (28.9 ± 2.2 mm) in human and animal tissues. Longer incubation times and incubation temperatures higher than 37C did not result in longer tracing distances. No differences were evident between adult and newborn animals and between central and peripheral nervous system. The diffusion coefficient for DiI was calculated to be 2.5 × 10-7 cm2sec-1. After application of DiI to nerves of guinea pig extraocular muscles, DiI-positive afferent perikarya were observed in the anteromedial part of the trigeminal ganglion. These perikarya were identified by calcitonin gene-related peptide immunoreactivity (CGRP-IR). The percentage of CGRP-IR neurons after tracing was concordant with the percentage of CGRP-IR in trigeminal ganglia exclusively processed for CGRP-IR without previous postmortem tracing. These results demonstrate carbocyanines to be specific tracers for exact neuronal mapping studies.

Fluorescent Neuronal Tracers

1984 ◽  
pp. 307-340 ◽  
Author(s):  
H.G.J.M. Kuypers ◽  
A.M. Huisman
Keyword(s):  
Neuronal Tracers

scholarly journals Multicolor Polymeric Nanoparticle Neuronal Tracers

2020 ◽  
Vol 6 (3) ◽  
pp. 436-445
Author(s):  
Nanzhi Zang ◽  
John B. Issa ◽  
Treffly B. Ditri ◽  
Dante S. Bortone ◽  
Mollie A. Touve ◽  
...  
Keyword(s):  
Polymeric Nanoparticle ◽  
Neuronal Tracers

Morphological analysis of mechanoreceptors in the rat skin identified by intraganglionic injections of neuronal tracers

2010 ◽  
Vol 68 ◽  
pp. e158
Author(s):  
Satomi Ebara ◽  
Knarik Bagdasarian ◽  
Takahiro Fruta ◽  
Taro Koike ◽  
Inbal Meir ◽  
...  
Keyword(s):  
Morphological Analysis ◽  
Rat Skin ◽  
Neuronal Tracers

P3.3 A new surgical approach for applying neuronal tracers on the rat heart

2009 ◽  
Vol 149 (1-2) ◽  
pp. 83
Author(s):  
I. Grković ◽  
V. Košta ◽  
M. Marinović Guić ◽  
A. Čarić
Keyword(s):  
Surgical Approach ◽  
Rat Heart ◽  
Neuronal Tracers
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