scholarly journals First report of Sweet potato virus G in sweet potato in Italy

2021 ◽  
Vol 44 (2) ◽  
Author(s):  
G. Parrella ◽  
E. Troiano
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
First Report ◽  
Sweet Potato Virus

scholarly journals First Report of Sweet potato virus G and Sweet potato virus 2 Infecting Sweetpotato in North Carolina

Plant Disease ◽  
2013 ◽  
Vol 97 (11) ◽  
pp. 1516-1516 ◽  
Author(s):  
C. V. Almeyda ◽  
J. A. Abad ◽  
Z. Pesic-VanEsbroeck
Keyword(s):  
North Carolina ◽  
Sweet Potato ◽  
Potato Virus ◽  
Simultaneous Detection ◽  
The United States ◽  
Rt Pcr ◽  
Indicator Plants ◽  
First Report ◽  
Sweet Potato Virus ◽  
Experimental Plots

Sweet potato virus G (SPVG) and Sweet potato virus 2 (SPV2) are two members of the genus Potyvirus, distinct from Sweet potato feathery mottle virus (SPFMV) (1,2,4). The significance of SPVG and SPV2 to sweetpotato (Ipomoea batatas Lam.) is that each virus can synergistically interact with Sweet potato chlorotic stunt virus (SPCSV) inducing sweet potato virus disease (SPVD) (1,2,4). During the summer of 2012, susceptible indicator plants (I. setosa) were evenly distributed in sweetpotato experimental plots at two research stations (Clinton and Kinston) in North Carolina (NC). Naturally infected indicator plants (n = 129) showing virus-like symptoms including vein clearing, chlorotic mosaic, and chlorotic spots were collected and tested for the presence of viruses. Sap extract from plants tested positive for SPVG and SPV2 by nitrocellulose immune-dot blot, using SPVG antiserum obtained from the International Potato Center (Lima, Peru) and SPV2 antiserum kindly provided by C. A. Clark, Louisiana State University. Total RNA was extracted from 200 mg of symptomatic leaf tissue by using the QIAGEN RNeasy Plant Mini Kit (Hilden, Germany) adding 2% PVP-40 and 1% 2-mercaptoethanol to the extraction buffer. Multiplex RT-PCR was carried out using the SuperScript III One-Step RT-PCR System (Invitrogen, Carlsbad, CA) with specific primers designed for simultaneous detection and differentiation of four closely related sweetpotato potyviruses (3). Amplicons were cloned using the pGEM-T Easy cloning kit (Promega, Madison, WI) and sequenced. Quantitative RT-PCR was used for SPCSV detection. Results confirmed the presence of SPVG and SPV2 in single infections on 7% and 0.8% of samples, respectively; and in mixed infections on 54% and 3% of samples, respectively. SPVG was found as the most prevalent in all viral combinations where 14% of samples were infected with SPVG and SPFMV; and 15% of samples were infected with SPVG, SPFMV, and Sweet potato virus C (SPVC). SPV2 was detected in less common combinations (0.8%) associated with SPVG and SPFMV. The mixed infection SPVG and SPCSV as well as the combination SPV2 and SPCSV was detected in 0.8% of samples. Sequence analyses of the samples at nucleotide level (GenBank Accession Nos. KC962218 and KC962219, respectively) showed 99% similarity to SPVG isolates from Louisiana (4) and SPV2 isolates from South Africa (1). Scions from infected indicator plants were wedge grafted onto healthy sweetpotatoes (cvs. Beauregard and Covington). Eight weeks after grafting, chlorotic mosaic was observed on plants with mixed potyvirus infections whereas plants with single potyvirus infection showed no obvious symptoms. RT-PCR testing and sequencing of amplicons corroborate the presence of both viruses initially detected in indicator plants. Additionally, naturally infected sweetpotato samples (n = 102) were collected in the same experimental plots. SPVG and SPV2 were detected and identified following the described methodology. In the United States, SPVG has been shown to be prevalent in Louisiana (4) and the results presented here indicate that SPVG is spreading in NC. Our results confirm the presence of SPVG and SPV2 in NC. To our knowledge, this is the first report of SPVG and SPV2 in sweetpotato fields in NC. References: (1) E. M. Ateka et al. Arch Virol 152:479, 2007. (2) F. Li et al. Virus Genes 45:118, 2012. (3) F. Li et al. J. Virol. Methods 186:161, 2012. (4) E. R. Souto et al. Plant Dis 87:1226, 2003.

scholarly journals First Report of Sweet potato golden vein associated virus Infecting Sweet Potato in Korea

Plant Disease ◽  
2014 ◽  
Vol 98 (8) ◽  
pp. 1163-1163
Author(s):  
E.-J. Kil ◽  
J. Kim ◽  
H.-S. Byun ◽  
H.-R. Kwak ◽  
M.-K. Kim ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
Leaf Roll ◽  
The United States ◽  
Viral Gene ◽  
Sweet Potatoes ◽  
Potato Leaf ◽  
Viral Dna ◽  
First Report ◽  
Sweet Potato Virus

Sweet potato (Ipomoea batatas) is one of the most important crops in eastern Asia, including Korea. Consumption of sweet potato is increasing gradually because of its growing reputation as a health food. Recently, outbreaks of viruses infecting sweet potatoes have increased all over the world, probably because sweet potatoes are produced via vegetative propagation (1,2). In Korea, most sweet potatoes in fields have been infected by a begomovirus, Sweet potato leaf curl virus (SPLCV), and other viruses such as Sweet potato feathery mottle virus, Sweet potato virus G, and Sweet potato latent virus (3). Many countries have monitored sweet potato virus infections in fields as well as in germplasm collections to select virus-free stocks. In 2013, 20 sweet potato plants showing leaf roll symptoms in Muan, South Korea, were collected and analyzed. Total DNA was isolated from sweet potato leaves (Viral Gene-spin Viral DNA/RNA Extraction Kit, iNtRON Biotechnology, Seongnam, Korea) and viral DNA was amplified by rolling circle amplification (RCA, TempliPhi Amplification Kit, GE Healthcare Life Sciences, Uppsala, Sweden) following the manufacturer's instructions. Amplicons were digested by restriction enzyme SacI (TaKaRa Bio, Shiga, Japan) and products were run on a 1.5% agarose gel. A 2.8-kb DNA fragment was purified from a gel, ligated into a pGEM-T easy vector (Promega, Madison, WI), and sequenced (Macrogen, Seoul, Korea). Based on a BLAST search, most of the sequences (36/38) were identified as SPLCV, but two independent clones 2,824 nt in length from sweet potato cv. Sincheonmi were similar to Sweet potato golden vein associated virus (SPGVaV) isolate US:MS:1B-3 (94.38%, GenBank Accession No. HQ333143). The complete genome sequence of the SPGVaV-Korea isolate contained six ORFs, as expected for a typical monopartite begomovirus. The sequence was deposited in GenBank under accession number KF803170. SPGVaV is a whitefly (Bemisia tabaci)-transmitted virus (genus Begomovirus, family Geminiviridae). A phylogenetic analysis that included other begomoviruses that infect sweet potato showed SPGVaV-Korea to segregate with other SPGVaV isolates. SPGVaV has previously only been reported in Brazil and the United States (1). This is the first report of SPGVaV in sweet potato outside of the Americas. References: (1) L. C. Albuquerque et al. Virol. J. 9:241, 2012. (2) E. Choi et al. Acta Virol. 56:187, 2012. (3) H. R. Kwak et al. Plant Pathol. J. 22:239, 2006.

scholarly journals First Report of Sweet potato virus G Infecting Sweet Potato in Taiwan

Plant Disease ◽  
2013 ◽  
Vol 97 (9) ◽  
pp. 1260-1260 ◽  
Author(s):  
L.-Y. Wang ◽  
Y.-H. Cheng ◽  
N.-Y. Wang ◽  
K.-C. Chen ◽  
S.-D. Yeh
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
Ipomoea Batatas ◽  
Agricultural Research ◽  
The United States ◽  
French Polynesia ◽  
Nucleotide Identity ◽  
Yield Reduction ◽  
First Report ◽  
Sweet Potato Virus

Sweet potato, Ipomoea batatas (L.) Lam., is an important root crop grown mainly in the counties of Changhua, Yunlin, Tainan, and Pingtung in Taiwan where Sweet potato feathery mottle virus (SPFMV) and Sweet potato latent virus (SPLV) have been reported. Commercial sweet potato grown in Nantou in 2009 and in Hualian in 2010 exhibited downward leaf curling and vein clearing, indicative of viral infection, yet symptoms were distinct from those caused by SPFMV, SPLV, or mixed infection of both viruses. Total RNA was extracted from two symptomatic plants from each county with RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and analyzed by reverse transcription (RT)-PCR using the potyvirusdegenerate primer Hrp5 (1) and oligo-dT18 with BamHI site at the 5′ end (5′-GGATCCTTTTTTTTTTTTTTTTTT-3′). Two healthy plants served as negative controls. An approximately 1.5-kb amplicon covering the region from the 3′-end of the nuclear inclusion protein b (NIb) gene to the 3′-untranslated region (3′-UTR) was amplified from all symptomatic plants, while the healthy controls remained negative. Subsequently, one sample from each location was cloned and sequenced (GenBank Accession No. HQ171932-TW1 [Nantou] and JN205346-TW2 [Hualian]). Based on sequence comparison, the two isolates shared only 86.7% nucleotide identity. BLAST analysis of the CP gene of the isolate TW1 revealed 99% nucleotide identity with the corresponding sequence of Sweet potato virus G (SPVG)-CH2 from China (Z83314). Isolate TW2, however, only shared 86% nucleotide identity with SPVG-CH2, indicating isolate TW2 is genetically different from other isolates and probably represents a new strain of SPVG. The presence of SPVG was further confirmed in symptomatic plants by indirect ELISA using SPVG antiserum developed by Y.-H. Cheng of the Agricultural Research Institute. Since co-infection of different viruses in sweet potato can cause severe leaf symptoms and significant yield reduction (3), a preliminary field survey was also conducted to determine the extent of co-infection with more than one potyvirus using three different primer pairs, SPVGup (5′-ACCGAGCTTTACCCCAGGTAGAGAG-3′)/SPVdw (5′-CGCGCAAGACTCATRTCAGTCAAAT-3′) for SPVG, FM16 (5′-GAATTTAAAGATGCAGGTGTGAAC-3′)/FM895 (5′-GAGGTTATGTATATTTCTAGTAAC-3′) for SPFMV, and L166 (5′-GACAGAGATATCAACACTGGCACC-3′)/L841 (5′-TCCAAGTAGTGTGTGTATGTTCCG-3′) for SPLV. Forty-six of 128 (36%) sweet potato samples collected from Nantou, Hualian, Yunlin, Tainan, and Chiayi counties during 2010 and 2011 tested positive for SPVG. Of the 46 samples that tested positive for SPVG, six were co-infected with SPLV, 19 were co-infected with SPFMV, and two were co-infected with all three viruses. Of the samples that tested negative for SPVG, 10 were infected with SPLV, eight were infected with SPFMV, and two were infected with both SPLV and SPFMV. To date, SPVG has been detected in China, the United States, Peru, Egypt, Ethiopia, Zimbabwe, South Africa, Spain, Java, New Zealand, Hawaii, French Polynesia, and Easter Island (2). To our knowledge, this is the first report of SPVG infecting sweet potato in Taiwan. SPVG could become a new and potentially serious threat to sweet potato production in Taiwan. References: (1) C. C. Chen et al. Bot. Stud. 47:369, 2006. (2) M. Rännäli et al. Plant Dis. 92:1313, 2008. (3) M. Untiveros et al. Plant Dis. 91:669, 2007.

Control strategies for sweet potato virus disease in Africa

2004 ◽  
Vol 100 (1) ◽  
pp. 115-122 ◽  
Author(s):  
Richard W. Gibson ◽  
Valentine Aritua ◽  
Emmanuel Byamukama ◽  
Isaac Mpembe ◽  
James Kayongo
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
Virus Disease ◽  
Control Strategies ◽  
Potato Virus Disease ◽  
Sweet Potato Virus ◽  
Sweet Potato Virus Disease

scholarly journals The Role of Aphid Abundance, Species Diversity, and Virus Titer in the Spread of Sweetpotato Potyviruses in Louisiana and Mississippi

Plant Disease ◽  
2013 ◽  
Vol 97 (1) ◽  
pp. 53-61 ◽  
Author(s):  
E. N. Wosula ◽  
J. A. Davis ◽  
C. A. Clark ◽  
T. P. Smith ◽  
R. A. Arancibia ◽  
...  
Keyword(s):  
Species Diversity ◽  
Virus Infection ◽  
Sweet Potato ◽  
Potato Virus ◽  
Virus Titer ◽  
The United States ◽  
Aphid Species ◽  
Sentinel Plants ◽  
Sweet Potato Virus ◽  
Aphid Abundance

Sweet potato feathery mottle virus (SPFMV), Sweet potato virus G (SPVG), and Sweet potato virus 2 (SPV2) are sweetpotato (Ipomoea batatas) potyviruses nonpersistently transmitted by aphids. Our objective was to determine how aphid abundance, aphid species diversity, and virus titers relate to the spread of SPFMV, SPVG, and SPV2 in Louisiana and Mississippi sweetpotato fields. The most abundant aphid species were Aphis gossypii, Myzus persicae, Rhopalosiphum padi, and Therioaphis trifolii. Aphids were captured during the entire crop cycle but virus infection of sentinel plants occurred mainly during the months of June to August. SPFMV was more commonly detected than SPVG or SPV2 in sentinel plants. Virus titers for SPFMV were higher in samples beginning in late June. Because significant aphid populations were present during April to June when virus titers were low in sweetpotato and there was very little virus infection of sentinel plants, low virus titers may have limited aphid acquisition and transmission opportunities. This is the first study to comprehensively examine aphid transmission of potyviruses in sweetpotato crops in the United States and includes the first report of R. maidis and R. padi as vectors of SPFMV, though they were less efficient than A. gossypii or M. persicae.

scholarly journals Identification of Sweet potato virus G isolated from sweet potato in Japan

2009 ◽  
Vol 75 (2) ◽  
pp. 102-108 ◽  
Author(s):  
S. YAMASAKI ◽  
J. SAKAI ◽  
S. KAMISOYAMA ◽  
K. HANADA
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
Sweet Potato Virus

scholarly journals Evaluation of Selected Zambian Popular Sweet Potato Genotypes for Response to Sweet Potato Virus Disease

2020 ◽  
pp. 44-51
Author(s):  
Joseph Banda ◽  
Patrick Chiza Chikoti ◽  
Langa Tembo
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
Virus Disease ◽  
Beta Carotene ◽  
Yield Reduction ◽  
Tuber Weight ◽  
Carotene Content ◽  
Potato Virus Disease ◽  
Sweet Potato Virus ◽  
Sweet Potato Virus Disease

Aim: The objective of this study was to determine the effect of sweet potato virus disease (SPVD) on the beta carotene content, tuber weight and vine weight of selected popular sweet potato genotypes. Study Design: The experiment was laid as a randomized complete block design (RCBD) with three replications. Place and Duration of Study: The experiment was conducted for two cropping seasons (2015/16 and 2016/17) at the Zambia Agriculture Research Institute in Chilanga district of Zambia. Methodology: The uninfected (control) genotypes of Kanga, Chiwoko and Chingovwa were evaluated alongside their SPVD infected genotypes. Genotypic infection was confirmed using molecular approaches, and data was collected at harvest on beta carotene content, tuber weight and vine weight. Results: The results showed that SPVD affects the yield and beta carotene content of sweet potato. Significant differences (P< .001) for yield performance and beta carotene were observed. The yield reduction in percentage across seasons for all genotypes between the uninfected and infected genotypes ranged from 77% to 79% and 67% to 76% for tuber weight and vine weight respectively. Only Chiwoko exhibited higher levels of beta carotene among the genotypes. However, the SPVD infected Chiwoko genotype compared to the uninfected treatment produced mean beta carotene content of 39.1 µg/g and 91.5 µg/g respectively. Conclusion: SPVD reduces the tuber weight, vine weight and beta carotene content in infected sweet potato genotypes.

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