scholarly journals First report of Diplodia africana from Fraxinus excelsior in New Zealand

2021 ◽  
Vol 44 (2) ◽  
Author(s):  
K. Dobbie ◽  
J. Baskarathevan ◽  
T. Waters ◽  
R. O'Neill
Keyword(s):  
New Zealand ◽  
Fraxinus Excelsior ◽  
First Report

Theileria orientalis, a blood parasite of cattle. first report in New Zealand

1984 ◽  
Vol 32 (9) ◽  
pp. 154-156 ◽  
Author(s):  
M.P. James ◽  
B.W. Saunders ◽  
Leslie A. Guy ◽  
E.O. Brookbanks ◽  
W.A.G. Charleston ◽  
...  
Keyword(s):  
New Zealand ◽  
Blood Parasite ◽  
Theileria Orientalis ◽  
First Report

scholarly journals First Report of Alternaria carotiincultae on Carrot Seed Produced in New Zealand

Plant Disease ◽  
2010 ◽  
Vol 94 (9) ◽  
pp. 1168-1168
Author(s):  
R. S. Trivedi ◽  
J. G. Hampton ◽  
J. M. Townshend ◽  
M. V. Jaspers ◽  
H. J. Ridgway
Keyword(s):  
New Zealand ◽  
Morphological Characteristics ◽  
New Taxon ◽  
First Report ◽  
Agar Plug ◽  
Carrot Seed ◽  
Agar Media ◽  
Leaves And Roots ◽  
Seed Crops ◽  
Β Tubulin

Carrot (Daucus carota L.) seed lots produced in Canterbury, New Zealand are commonly infected by the fungal pathogen Alternaria radicina, which can cause abnormal seedlings and decayed seeds. In 2008, samples of 400 seeds from each of three carrot seed crops were tested for germination on moistened paper towels. On average, 30% of the seeds developed into abnormal seedlings or were decayed and were plated onto A. radicina selective agar (2) and acidified potato dextrose agar media and grown for 15 days at 22°C (10 h/14 h light/dark cycle) to confirm the presence of this pathogen (3). However, another fungus was isolated from an average of 8% of the seeds sampled. Colonies of the latter fungus grew faster than those of A. radicina, had smoother margins, and did not produce dendritic crystals or yellow pigment in the agar media. Although conidial size (30 to 59 × 18 to 20 μm), shape (long and ellipsoid), and color (dark olive-brown) were similar for the two fungi, conidia of this novel fungus had more transverse septa (average 3.6 cf. 3.0 per conidium) than those of A. radicina. On the basis of these morphological characteristics, the isolated fungus was identified as A. carotiincultae and the identity was confirmed by sequence analysis. PCR amplification of the β-tubulin gene from three isolates, using primers Bt1a (5′ TTCCCCCGTCTCCACTTCTTCATG 3′) and Bt1b (5′ GACGAGATCGTTCATGTTGAACTC 3′) (1), produced a 420-bp product for each isolate that was sequenced and compared with β-tubulin sequences present in GenBank. Sequences of all three New Zealand isolates (Accession Nos. HM208752, HM208753, and HM208754) were identical to each other and to six sequences in GenBank (Accession Nos. EU139354/57/58/59/61/62). There was a 2- to 4-bp difference between these sequences and those of A. radicina present in GenBank. Pathogenicity of the three New Zealand isolates of A. carotiincultae was verified on leaves and roots of 3-month-old carrot plants grown in a greenhouse (three plants per pot with 10 replicate pots per isolate). For each isolate, intact leaves of each plant were inoculated with 0.5 ml of a suspension of 106 conidia/ml and the tap root of each plant was inoculated with a 7-mm agar plug colonized by the isolate. Ten pots of control plants were treated similarly with sterile water and noncolonized agar plugs. Each pot was covered with a plastic bag for 12 h and then placed in a mist chamber in a greenhouse with automatic misting every 30 min. At 72 h after inoculation, symptoms comprising medium brown-to-black lesions on the leaves and dark brown-to-black sunken lesions on the roots were clearly visible on inoculated plants but not on the control plants. Reisolation attempts from roots and leaves demonstrated A. carotiincultae to be present in symptomatic leaves and roots of all inoculated plants but not in leaves or roots of the control plants. Symptoms produced by the isolates of A. carotiincultae were similar to those attributed to A. radicina in infected carrot seed fields in Canterbury. The former species may have caused field infections in carrot seed crops in Canterbury. A. carotiincultae was described as a new taxon in Ohio in 1995 (4), and pathogenicity of the species on carrot was reported in California (3). To our knowledge, this is the first report of A. carotiincultae in New Zealand. References: (1) M. S. Park et al. Mycologia 100:511, 2008. (2) B. M. Pryor et al. Plant Dis. 78:452, 1994. (3) B. M. Pryor and R. L. Gilbertson. Mycologia 94:49, 2002. (4) E. G. Simmons. Mycotaxon 55:55, 1995.

scholarly journals First Report of Spiranthes mosaic virus 3 in Phlox spp. in New Zealand

Plant Disease ◽  
2017 ◽  
Vol 101 (5) ◽  
pp. 849-849 ◽  
Author(s):  
J. Tang ◽  
S. Khan ◽  
B. Quinn ◽  
S. Veerakone ◽  
E. Milleza ◽  
...  
Keyword(s):  
New Zealand ◽  
Mosaic Virus ◽  
First Report

scholarly journals First Report of Turnip yellows virus in Daphne odora in New Zealand

Plant Disease ◽  
2018 ◽  
Vol 102 (7) ◽  
pp. 1467-1467
Author(s):  
S. Veerakone ◽  
J. Tang ◽  
A. Zheng ◽  
L. I. Ward
Keyword(s):  
New Zealand ◽  
First Report ◽  
Turnip Yellows Virus ◽  
Daphne Odora

scholarly journals First Report of Phragmidium violaceum Infecting Himalaya and Evergreen Blackberries in North America

2005 ◽  
Vol 6 (1) ◽  
pp. 25 ◽  
Author(s):  
N. Osterbauer ◽  
A. Trippe ◽  
K. French ◽  
T. Butler ◽  
M. C. Aime ◽  
...  
Keyword(s):  
South Africa ◽  
Biological Control ◽  
New Zealand ◽  
North America ◽  
Biological Control Agent ◽  
Control Agent ◽  
First Report ◽  
Official Report

Phragmidium violaceum occurs on several species of Rubus, including R. armeniacus, R. fruticosus agg., and R. laciniatus, in Europe, South Africa, Iran, and Iraq, and has been introduced as a biological control agent for invasive blackberries in Australia, New Zealand, and Chile. To our knowledge, this is the first official report of P. violaceum infecting Himalaya and evergreen blackberries in North America. Accepted for publication 16 September 2005. Published 23 September 2005.

First report of Eimeria leuckarti infection in a horse in New Zealand

1982 ◽  
Vol 30 (7) ◽  
pp. 104-105 ◽  
Author(s):  
A. C. Johnstone ◽  
H. G. Pearce ◽  
W. A. G. Charleston
Keyword(s):  
New Zealand ◽  
First Report

scholarly journals First Report of a Carlavirus in Fuchsia spp. in New Zealand

Plant Disease ◽  
2011 ◽  
Vol 95 (11) ◽  
pp. 1484-1484 ◽  
Author(s):  
Z. Perez-Egusquiza ◽  
L. W. Liefting ◽  
S. Veerakone ◽  
G. R. G. Clover ◽  
M. Ciuffo
Keyword(s):  
Electron Microscopy ◽  
New Zealand ◽  
Coat Protein ◽  
Chenopodium Quinoa ◽  
Plant Diseases ◽  
Nucleotide Identity ◽  
Impatiens Necrotic Spot Virus ◽  
Rt Pcr ◽  
First Report ◽  
Genome Region

The genus Fuchsia has 110 known species and numerous hybrids. These ornamental plants with brightly colored flowers originate from Central and South America, New Zealand, and Tahiti, but a wider variety are now grown all over the world. Few viruses have been reported in Fuchsia spp.: a carlavirus, Fuchsia latent virus (FLV) (1–3), a cucumovirus, Cucumber mosaic virus (CMV) (3), and two tospoviruses, Impatiens necrotic spot virus (INSV) and Tomato spotted wilt virus (TSWV) (4). In August 2009, five plants, each representing a different cultivar of Fuchsia hybrid, from home gardens in the Auckland and Southland regions of New Zealand, displayed variable symptoms including mild chlorosis, mild mottle, or purple spots on leaves. Plants tested negative for CMV, INSV, and TSWV using commercial ImmunoStrips (Agdia Inc., Elkhart, IN); however, flexuous particles of ~650 to 700 nm were found by electron microscopy in all samples. Local lesions were also observed on Chenopodium quinoa plants 4 weeks after sap inoculation. Total RNA was extracted from all plants with a RNeasy Plant Mini Kit (Qiagen Inc., Doncaster, Australia) and tested by reverse transcription (RT)-PCR using two generic sets of primers (R. van der Vlugt, personal communication) designed to amplify fragments of ~730 and 550 bp of the replicase and coat protein genes of carlaviruses, respectively. Amplicons of the expected size were obtained for all samples, cloned, and at least three clones per sample were sequenced. No differences within clones from the same samples were observed (GenBank Accession Nos. HQ197672 to HQ197681). A BLASTn search of the viral replicase fragment showed the highest nucleotide identity (76%) to Potato rough dwarf virus (PRDV) (EU020009), whereas the coat protein fragment had maximum nucleotide identity (70 to 72%) to PRDV (EU020009 and DQ640311) and Potato virus P (DQ516055). Sequences obtained were also pairwise aligned using the MegAlign program (DNASTAR, Inc., Madison, WI) and results showed that the isolates had 83 to 97% identity to each other within each genome region. Further sequences (HQ197925 and HQ197926) were obtained from a Fuchsia plant originating from Belgium, a BLASTn analysis showed high nucleotide identity (84 to 99%) to the New Zealand isolates. The low genetic identity to other Carlavirus members suggests that these isolates belong to a different species from those previously sequenced. On the basis of electron microscopy and herbaceous indexing, the isolates had similar characteristics to a carlavirus reported from Fuchsia in Italy (1) and FLV reported in Canada (2). The Italian carlavirus isolate was obtained and tested with the same primers by RT-PCR. Pairwise analysis of the Italian sequences (HQ197927 and HQ197928) with the New Zealand and Belgian sequences showed between 84 and 95% similarity within each genome region. These results suggest that the carlavirus infecting these plants is the same virus, possibly FLV. To our knowledge, this is the first report of this carlavirus infecting Fuchsia spp. in New Zealand, but the virus has probably been present for some time in this country and is likely to be distributed worldwide. References: (1) G. Dellavalle et al. Acta Hortic. 432:332, 1996. (2) L. J. John et al. Acta Hortic. 110:195, 1980. (3) P. Roggero et al. Plant Pathol. 49:802, 2000. (4) R. Wick and B. Dicklow. Diseases in Fuchsia. Common Names of Plant Diseases. Online publication. The American Phytopathological Society, St. Paul, MN, 1999.

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