scholarly journals First report of Pineapple mealybug wilt‐associated virus‐1 and ‐2 associated with mealybug wilt disease of pineapple in Mauritius

2021 ◽  
Vol 44 (1) ◽  
Author(s):  
A. Gungoosingh‐Bunwaree ◽  
F. Maudarbaccus ◽  
D. Knierim ◽  
P. Margaria ◽  
S. Winter ◽  
...  
Keyword(s):  
Wilt Disease ◽  
First Report ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

scholarly journals First Report of a Closterovirus-Like Particle Associated with Pineapple Plants (Ananas comosus cv. Smooth Cayenne) Affected with Pineapple Mealybug Wilt in Cuba

Plant Disease ◽  
1998 ◽  
Vol 82 (2) ◽  
pp. 263-263 ◽  
Author(s):  
Eduviges G. Borroto ◽  
Mayra Cintra ◽  
Justo González ◽  
Carlos Borroto ◽  
Pedro Oramas
Keyword(s):  
Electron Microscope ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
System Size ◽  
Ananas Comosus ◽  
Meristem Culture ◽  
First Report ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

Pineapple mealybug wilt (PMW) was first described in Hawaii in 1910 and has since spread wherever pineapple is cultivated. PMW is currently one of the most important field diseases of pineapple in Cuba, causing up to 40% crop loss. Leaves of plants affected with PMW turn bronze-red and lose turgidity, and the tips curve down. Root system size is decreased and fruits are unmarketable because the flesh is fibrous and sour. Leaves from wilt-affected pineapple plants were selected from the Experimental Station “Tomás Roig” at Ciego de Avila, Cuba. Asymptomatic plants derived from meristem tissue and cultivated under greenhouse conditions were also collected. Both samples were analyzed by several virus purification procedures and finally purified with a protocol modified from Gunashinge and German (1). Partially purified samples from symptomatic and asymptomatic plants were negatively stained and examined by a JEOL (JEM-2000 EX) transmission electron microscope at a magnification of ×30,000. Sixteen, long, flexuous, rod-shaped, viruslike particles (estimated length 1,200 to 1,450 nm; width about 12 nm) were observed in symptomatic leaves, using electron microscope micrographs. These particles were similar to those of the pineapple clostero-like virus found in Hawaii (1) and Australia (2). Particles were observed from symptomatic plants but not from plants propagated by primary meristem culture. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of viral proteins indicated that the coat protein has a molecular mass of approximately 23 kDa. This is the first report of closterovirus-like particles associated with pineapple plants (Ananas comosus cv. Smooth Cayenne) affected with PMW in Cuba. References: (1) U. B. Gunasinge and T. L. German. Phytopathology 79:1337, 1989. (2) W. Wakman et al. Aust. J. Agric. Res. 46:947, 1995.

scholarly journals First Report of Three Pineapple Mealybug Wilt-Associated Viruses in Queen Victoria Pineapples in Reunion Island

Plant Disease ◽  
2021 ◽  
pp. PDIS-05-20-1068
Author(s):  
D. Massé ◽  
N. Cassam ◽  
B. Hostachy ◽  
M.-L. Iskra-Caruana ◽  
M. Darnaudery ◽  
...  
Keyword(s):  
Reunion Island ◽  
First Report ◽  
Queen Victoria ◽  
Réunion Island ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

scholarly journals Spatial and Temporal Incidences of Pineapple mealybug wilt-associated viruses in Pineapple Planting Blocks

Plant Disease ◽  
2010 ◽  
Vol 94 (2) ◽  
pp. 196-200 ◽  
Author(s):  
D. M. Sether ◽  
W. B. Borth ◽  
M. J. Melzer ◽  
J. Hu
Keyword(s):  
Ananas Comosus ◽  
Ratoon Crop ◽  
Virus Incidence ◽  
Central Regions ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug ◽  
Fruit Harvest ◽  
Propagation Material

A complex of Pineapple mealybug wilt-associated viruses (PMWaVs) that can infect pineapple (Ananas comosus) is correlated with reduced yields and mealybug wilt of pineapple. The incidences of PMWaV-1 and PMWaV-2 at planting, fruit harvest, the beginning of the ratoon crop, and ratoon fruit harvest were determined for end, side, and central regions of planting blocks in eight commercial fields. Differences in virus incidence for the three regions at ratoon harvest were highly significant (P = 0.0018). Central regions of planting blocks had lower virus incidences at the time of ratoon fruit harvest. Collection of propagation material from the central regions of planting blocks will help to minimize PMWaV incidence in fields planted with this material.

scholarly journals Probing behavior of Dysmicoccus brevipes mealybug in pineapple plants1

2016 ◽  
Vol 46 (4) ◽  
pp. 458-463
Author(s):  
Lenira Viana Costa Santa-Cecília ◽  
◽  
Ernesto Prado ◽  
Brígida Souza ◽  
Keyword(s):  
Insect Vector ◽  
Recording Time ◽  
Defense Strategies ◽  
Phenolic Contents ◽  
Probing Behavior ◽  
Epg Technique ◽  
Dysmicoccus Brevipes ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

ABSTRACT Differences in susceptibility to viruses in plants may be partially explained by the insect vector probing behavior and by the presence of phenolic compounds, which are often associated with defense strategies. This study aimed at detecting barriers that may difficult the probing activity of the Dysmicoccus brevipes (Pseudococcidae) pineapple mealybug, a vector of the pineapple mealybug wilt-associated virus, as well as evaluating the phenolic content of plants, in order to verify any possible relationship with the probing behavior, by using the electrical penetration graphs (EPG) technique. Seedlings of 'Smooth Cayenne' and 'Pérola' pineapple cultivars were used in the experiments. Only 28 % and 21 % of the mealybugs reached the phloem of the 'Smooth Cayenne' and 'Pérola' cultivars, respectively, over 16 h of recording, with an average of 9 h to reach the phloem vessels. The xylem phase was extended in both cultivars and represented approximately 31 % ('Smooth Cayenne') and 44 % ('Pérola') of the recording time. The phenolic contents of both cultivars were similar.

PINEAPPLE MEALYBUG WILT ASSOCIATED VIRUS AND MEALYBUG WILT OF PINEAPPLE

2005 ◽  
pp. 209-212 ◽  
Author(s):  
J.S. Hu ◽  
D.M. Sether ◽  
M.J. Metzer ◽  
E. Pérez ◽  
A. Gonsalves ◽  
...  
Keyword(s):  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug

Further genomic characterization of pineapple mealybug wilt-associated viruses using high-throughput sequencing

2020 ◽  
Vol 45 (1) ◽  
pp. 64-72 ◽  
Author(s):  
James C. Green ◽  
Maher A. Rwahnih ◽  
Alejandro Olmedo-Velarde ◽  
Michael J. Melzer ◽  
Islam Hamim ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Genomic Characterization ◽  
Mealybug Wilt ◽  
Pineapple Mealybug Wilt ◽  
Pineapple Mealybug
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