scholarly journals Comparison of in vivo and in situ detection of hippocampal metabolites in mouse brain using 1 H‐MRS

2020 ◽  
Vol 34 (2) ◽  
Author(s):  
Chao‐Hsiung Hsu ◽  
Stephen Lin ◽  
Ai‐Chen Ho ◽  
T. Derek Johnson ◽  
Paul C. Wang ◽  
...  
Keyword(s):  
Mouse Brain ◽  
In Situ Detection

Inside front cover: In situ detection of histone variants and modifications in mouse brain using imaging mass spectrometry

PROTEOMICS ◽  
2016 ◽  
Vol 16 (3) ◽  
pp. NA-NA
Author(s):  
Shibojyoti Lahiri ◽  
Na Sun ◽  
Victor Solis-Mezarino ◽  
Andreas Fedisch ◽  
Jovica Ninkovic ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Brain ◽  
Imaging Mass Spectrometry ◽  
Histone Variants ◽  
Front Cover ◽  
In Situ Detection

PRRSV: Study of in vivo cell tropism and virus-induced apoptosis by in situ detection techniques

2000 ◽  
Vol 31 (1) ◽  
pp. 58-59 ◽  
Author(s):  
J. H. Sur ◽  
A. R. Doster ◽  
J. Galeota ◽  
R. W. Wills ◽  
F. A. Osorio
Keyword(s):  
Cell Tropism ◽  
Detection Techniques ◽  
Induced Apoptosis ◽  
In Situ Detection

scholarly journals Conjugate formation in urea: coupling of insoluble peptides to alkaline phosphatase for ELISA and in situ detection of antibody-forming cells.

1991 ◽  
Vol 39 (7) ◽  
pp. 987-992 ◽  
Author(s):  
K Gerritse ◽  
M Fasbender ◽  
W Boersma ◽  
E Claassen
Keyword(s):  
Alkaline Phosphatase ◽  
Synthetic Peptide ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sufficient Degree ◽  
Antipeptide Antibody ◽  
In Situ Detection ◽  
Antibody Secreting Cells

We report here a new method to produce synthetic peptide/alkaline phosphatase (AP) conjugates in the presence of urea. The method allows the use of peptides that are not soluble to a sufficient degree in aqueous buffers. The presence of 8 M urea during the construction of the synthetic peptide/AP conjugates does not influence enzyme activity nor the affinity of the anti-peptide antibodies for the conjugated peptide. We demonstrate that these synthetic peptide/AP conjugates can be used for detection of specific antipeptide antibody-forming cells (AFC) in vivo. This method for constructing enzyme conjugates with insoluble proteins or peptides suggest not only new possibilities for detection of specific AFC in vivo but also for applications in receptor-ligand studies, ELISA (enzyme-linked immunosorbent assay), and spot ELISA for detection of antibody-secreting cells in vitro.

scholarly journals Genomic organization of mouse ZAKI-4 gene that encodes ZAKI-4 alpha and beta isoforms, endogenous calcineurin inhibitors, and changes in the expression of these isoforms by thyroid hormone in adult mouse brain and heart

2004 ◽  
pp. 371-380 ◽  
Author(s):  
Y Mizuno ◽  
Y Kanou ◽  
M Rogatcheva ◽  
T Imai ◽  
S Refetoff ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Mouse Brain ◽  
Human Fibroblasts ◽  
Calcineurin Inhibitors ◽  
Gene Organization ◽  
Cultured Human Fibroblasts ◽  
Human Chromosome 6 ◽  
Rapid Amplification

OBJECTIVE: ZAKI-4 was identified as a thyroid hormone-responsive gene in cultured human fibroblasts. A single ZAKI-4 gene encodes two isoforms, ZAKI-4 alpha and beta, both inhibiting calcineurin activity. ZAKI-4 alpha and beta differ at their N termini, and show distinct distribution profiles in human tissues. The aim of this study was to elucidate the organization of the mouse ZAKI-4 gene and to determine the effect of thyroid hormone on the expression of ZAKI-4 isoforms in vivo. DESIGN: We cloned mouse homologues of human ZAKI-4 alpha and beta cDNA. Fluorescence in situ hybridization and bioinformatics analysis were employed to determine the gene organization. The effect of thyroid hormone on the expression of ZAKI-4 isoforms in mouse brain and heart was also studied. METHODS: Total RNA extracted from mouse cerebellum was used to clone ZAKI-4 alpha and beta cDNAs by RT-PCR followed by rapid amplification of cDNA ends. Mice were rendered hypothyroid by feeding a low iodine diet supplemented with propylthiouracil for 2 weeks. In one group (hyperthyroid) L-T(3) was injected i.p. for the last 4 days whereas another group (hypothyroid) received vehicle only. Non-treated mice were controls. RESULTS AND CONCLUSION: Mouse ZAKI-4 alpha and beta cDNAs were highly homologous to the human isoforms. The gene was mapped on chromosome 17qC, syntenic to human chromosome 6 where the human ZAKI-4 gene is located. As observed in human, ZAKI-4 alpha mRNA was expressed only in brain whereas beta mRNA was distributed in other tissues as well, such as heart and skeletal muscle. ZAKI-4 alpha mRNA was lower in the cerebral cortex of hypothyroid mice. Injection of L-T(3) caused an increase in ZAKI-4 beta mRNA in heart; however, expression of neither ZAKI-4 alpha nor beta mRNA was influenced by thyroid status in other tissues. These results indicate that expression of ZAKI-4 alpha and beta isoforms is regulated by thyroid hormone in vivo, and the regulation is isoform- and tissue-specific.

scholarly journals In situ detection of histone variants and modifications in mouse brain using imaging mass spectrometry

PROTEOMICS ◽  
2016 ◽  
Vol 16 (3) ◽  
pp. 437-447 ◽  
Author(s):  
Shibojyoti Lahiri ◽  
Na Sun ◽  
Victor Solis-Mezarino ◽  
Andreas Fedisch ◽  
Jovica Ninkovic ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mouse Brain ◽  
Imaging Mass Spectrometry ◽  
Histone Variants ◽  
In Situ Detection

In Situ Detection of GAD mRNA in Mouse Brain

1986 ◽  
pp. 135-149 ◽  
Author(s):  
Carol W. Wuenschell ◽  
Robin S. Fisher ◽  
Niranjala J. K. Tillakaratne ◽  
Allen J. Tobin
Keyword(s):  
Mouse Brain ◽  
In Situ Detection
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