Renal parenchyma impairment characterization in partial unilateral ureteral obstruction in mice with intravoxel incoherent motion-MRI

2017 ◽  
Vol 31 (2) ◽  
pp. e3858 ◽  
Author(s):  
Maguelonne Pons ◽  
Benjamin Leporq ◽  
Liza Ali ◽  
Marianne Alison ◽  
Miguel Albuquerque ◽  
...  
Keyword(s):  
Ureteral Obstruction ◽  
Unilateral Ureteral Obstruction ◽  
Renal Parenchyma ◽  
Intravoxel Incoherent Motion ◽  
Motion Mri

Use of intravoxel incoherent motion MRI to assess renal fibrosis in a rat model of unilateral ureteral obstruction

2016 ◽  
Vol 44 (3) ◽  
pp. 698-706 ◽  
Author(s):  
Xiang-Ran Cai ◽  
Juan Yu ◽  
Qing-Chun Zhou ◽  
Bin Du ◽  
You-Zhen Feng ◽  
...  
Keyword(s):  
Rat Model ◽  
Ureteral Obstruction ◽  
Renal Fibrosis ◽  
Unilateral Ureteral Obstruction ◽  
Intravoxel Incoherent Motion ◽  
Motion Mri

scholarly journals Postobstructive regeneration of kidney is derailed when surge in renal stem cells during course of unilateral ureteral obstruction is halted

2010 ◽  
Vol 298 (2) ◽  
pp. F357-F364 ◽  
Author(s):  
H. C. Park ◽  
K. Yasuda ◽  
B. Ratliff ◽  
A. Stoessel ◽  
Y. Sharkovska ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ureteral Obstruction ◽  
Control Level ◽  
Unilateral Ureteral Obstruction ◽  
Renal Parenchyma ◽  
Hematopoietic Stem ◽  
Caveolin 1 ◽  
Renal Stem Cells ◽  
Cell Accumulation ◽  
Stromal Cell Derived Factor

Unilateral ureteral obstruction (UUO), a model of tubulointerstitial scarring (TIS), has a propensity toward regeneration of renal parenchyma after release of obstruction (RUUO). No information exists on the contribution of stem cells to this process. We performed UUO in FVB/N mice, reversed it after 10 days, and examined kidneys 3 wk after RUUO. UUO resulted in attenuation of renal parenchyma. FACS analysis of endothelial progenitor (EPC), mesenchymal stem (MSC) and hematopoietic stem (HSC) cells obtained from UUO kidneys by collagenase-dispersed single-cell suspension showed significant increase in EPC, MSC, and HSC compared with control. After RUUO cortical parenchyma was nearly restored, and TIS score improved by 3 wk. This reversal process was associated with return of stem cells toward baseline level. When animals were chronically treated with nitric oxide synthase (NOS) inhibitor at a dose that did not induce hypertension but resulted in endothelial dysfunction, TIS scores were not different from control UUO, but EPC number in the kidney decreased significantly; however, parenchymal regeneration in these mice was similar to control. Blockade of CXCR4-mediated engraftment resulted in dramatic worsening of UUO and RUUO. Similar results were obtained in caveolin-1-deficient but not -overexpressing mice, reflecting the fact that activation of CXCR4 occurs in caveolae. The present data show increase in EPC, HSC, and MSC population during UUO and a tendency for these cells to decrease to control level during RUUO. These processes are minimally affected by chronic NOS inhibition. Blockade of CXCR4-stromal cell-derived factor-1 (SDF-1) interaction by AMD3100 or caveolin-1 deficiency significantly reduced the UUO-associated surge in stem cells and prevented parenchymal regeneration after RUUO. We conclude that the surge in stem cell accumulation during UUO is a prerequisite for regeneration of renal parenchyma.

Evaluation of preoperative ultrasonographic parameters to predict renal recovery in long-term survivors after treatment of feline ureteral obstructions: 2012–2019

2021 ◽  
pp. 1098612X2110236
Author(s):  
Elisa P McEntee ◽  
Allyson C Berent ◽  
Chick Weisse ◽  
Alexandre Le Roux ◽  
Kenneth Lamb
Keyword(s):  
Serum Creatinine ◽  
Ultrasound Imaging ◽  
Ureteral Obstruction ◽  
Unilateral Ureteral Obstruction ◽  
Biochemical Data ◽  
Preoperative Imaging ◽  
Long Term Outcome ◽  
Preoperative Ultrasound ◽  
Imaging Characteristics

Objectives The aim of this study was to determine whether preoperative ultrasound imaging characteristic(s) in cats suffering from unilateral benign ureteral obstructions are predictive of outcome after successful renal decompression with a subcutaneous ureteral bypass (SUB) device. Methods This was a retrospective study of 37 cats with unilateral, benign ureteral obstruction. Preoperative imaging characteristics (including renal pelvis diameter, parenchymal thickness [transverse plane], renal length and pelvic size:overall renal size) and biochemical data were evaluated for all cats diagnosed with a unilateral ureteral obstruction treated with a SUB device. Any patient with bilateral obstructions or documented bacteriuria/infection in the data collection period was excluded. All patients were followed between 3 and 6 months postoperatively to obtain postoperative biochemical data. Long-term outcome was defined as serum creatinine concentration at 3–6 months postoperatively. Results No preoperative imaging characteristics or biochemical findings were found to be significantly associated with long-term serum creatinine concentrations. The length of the kidney was found to be associated with change in blood urea nitrogen and creatinine with decompression but not with long-term renal values. Conclusions and relevance In this study, long-term renal function based on preoperative ultrasound imaging findings could not be predicted in cats with unilateral ureteral obstruction, regardless of the severity of the biochemical parameters, renal pelvic dilation (large or small pelvis), kidney size or thickness of renal parenchyma assessed.

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