scholarly journals On the theoretical limits of detecting cyclic changes in cardiac high-energy phosphates and creatine kinase reaction kinetics using in vivo 31 P MRS

2015 ◽  
Vol 28 (6) ◽  
pp. 694-705 ◽  
Author(s):  
Kilian Weiss ◽  
Paul A. Bottomley ◽  
Robert G. Weiss
Keyword(s):  
Creatine Kinase ◽  
Reaction Kinetics ◽  
High Energy ◽  
High Energy Phosphates ◽  
Kinase Reaction ◽  
Creatine Kinase Reaction ◽  
Cyclic Changes

scholarly journals In vivo creatine kinase reaction kinetics at rest and stress in type II diabetic rat heart

2015 ◽  
Vol 3 (1) ◽  
pp. e12248 ◽  
Author(s):  
Adil Bashir ◽  
Andrew R. Coggan ◽  
Robert J. Gropler
Keyword(s):  
Creatine Kinase ◽  
Reaction Kinetics ◽  
Rat Heart ◽  
Diabetic Rat ◽  
Type Ii ◽  
Kinase Reaction ◽  
Creatine Kinase Reaction

Carbofuran-induced alterations (in vivo) in high-energy phosphates, creatine kinase (CK) and CK isoenzymes

1991 ◽  
Vol 65 (4) ◽  
pp. 304-310 ◽  
Author(s):  
Ramesh C. Gupta ◽  
John T. Goad ◽  
Wade L. Kadel
Keyword(s):  
Creatine Kinase ◽  
High Energy ◽  
High Energy Phosphates

scholarly journals Triple repetition time saturation transfer (TRiST) 31 P spectroscopy for measuring human creatine kinase reaction kinetics

2010 ◽  
Vol 63 (6) ◽  
pp. 1493-1501 ◽  
Author(s):  
Michael Schär ◽  
AbdEl-Monem M. El-Sharkawy ◽  
Robert G. Weiss ◽  
Paul A. Bottomley
Keyword(s):  
Creatine Kinase ◽  
Reaction Kinetics ◽  
Saturation Transfer ◽  
Kinase Reaction ◽  
Repetition Time ◽  
Creatine Kinase Reaction

Fish muscle energy metabolism measured during hypoxia and recovery: an in vivo 31P-NMR study

1995 ◽  
Vol 268 (5) ◽  
pp. R1178-R1187 ◽  
Author(s):  
V. van Ginneken ◽  
G. van den Thillart ◽  
A. Addink ◽  
C. Erkelens
Keyword(s):  
Creatine Kinase ◽  
Fish Muscle ◽  
High Energy ◽  
Anaerobic Glycolysis ◽  
O2 Consumption ◽  
Nmr Study ◽  
Creatine Kinase Equilibrium ◽  
Creatine Kinase Reaction ◽  
Muscle Energy Metabolism

Three fish species were exposed to graded hypoxia levels and allowed to recover. Levels of high-energy phosphate compounds in epaxial white muscle were monitored by in vivo 31P nuclear magnetic resonance (NMR) spectroscopy. Furthermore, O2 consumption of the animals was measured. With increasing hypoxia load, metabolic parameters started to change in the following order: phosphocreatine (PCr)-to-Pi ratio (decrease), O2 consumption (decrease), [PCr] (decrease), intracellular pH (pHi; decrease), Pi (increase), free ADP concentration ([ADP]free; increase), [ATP] (decrease). PCr levels fell with the PO2. After each increment, the [PCr] reached a stable plateau value while, in some cases, a recovery was observed. This recovery could be explained because the balance between anaerobic and aerobic metabolism is continuously fluctuating during hypoxia as a consequence of changes in the activity of the fish. Consequently the [ADP]free are fluctuating, resulting in an activation of the creatine kinase reaction and the anaerobic glycolysis. In all three species, anaerobic glycolysis was activated, but in contrast to anoxia exposure, metabolic suppression was absent. The changes of [ADP]free and [H+] (which influences the position of the creatine kinase equilibrium) are species dependent. Species differences observed in the other parameters were small. It is concluded that the pattern of the activation of anaerobic metabolism under deep hypoxia is different from that under anoxia.

scholarly journals Localized rest and stress human cardiac creatine kinase reaction kinetics at 3 T

2019 ◽  
Vol 32 (6) ◽  
pp. e4085 ◽  
Author(s):  
William T. Clarke ◽  
Mark A. Peterzan ◽  
Jennifer J. Rayner ◽  
Rana A. Sayeed ◽  
Mario Petrou ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Reaction Kinetics ◽  
Kinase Reaction ◽  
Creatine Kinase Reaction

scholarly journals Four-angle saturation transfer (FAST) method for measuring creatine kinase reaction rates in vivo

2002 ◽  
Vol 47 (5) ◽  
pp. 850-863 ◽  
Author(s):  
Paul A. Bottomley ◽  
Ronald Ouwerkerk ◽  
Ray F. Lee ◽  
Robert G. Weiss
Keyword(s):  
Creatine Kinase ◽  
Reaction Rates ◽  
Fast Method ◽  
Saturation Transfer ◽  
Kinase Reaction ◽  
Creatine Kinase Reaction

scholarly journals Patterns in mammalian muscle energetics

1985 ◽  
Vol 115 (1) ◽  
pp. 165-177
Author(s):  
M. J. Kushmerick
Keyword(s):  
Creatine Kinase ◽  
Contractile Activity ◽  
High Energy ◽  
Cellular Respiration ◽  
High Energy Phosphate ◽  
Chemical Changes ◽  
Kinase Reaction ◽  
Slow Twitch ◽  
Creatine Kinase Reaction ◽  
Fast Twitch

A description of cellular energetics of muscular contraction is given in terms of the rates and extents of high-energy phosphate splitting during contractile activity, in terms of high-energy phosphate resynthesis by respiration and net anaerobic glycolysis, and in terms of the associated uptake and/or release of H+. These chemical changes have been studied quantitatively by rapid freeze-clamping methods and by 31P-NMR methods. The pattern of chemical changes in a fast-twitch glycolytic muscle is rapid depletion of phosphocreatine and later ATP levels, cellular acidification, and a much slower rate of resynthesis of high-energy phosphate compounds during the recovery period afterwards than occurs in the slow-twitch oxidative muscles. In steady-state contractile activity below the maximal, graded levels of high-energy phosphates and of cellular respiration are achieved in both fast-twitch and slow-twitch muscles. Within the metabolic range up to the maximal aerobic capacity, which differs several-fold for different fibre types, this gradation is mediated by the creatine kinase reaction and phosphocreatine stores. Thus while the amount of enzyme present and the content of phosphocreatine differs among muscles of different types, the same general energetic function is seen to occur in all muscle cells. The creatine kinase reaction is both an energy reservoir and a buffer preventing large swings in the ATP/ADP ratios.

scholarly journals 31 P magnetic resonance fingerprinting for rapid quantification of creatine kinase reaction rate in vivo

2017 ◽  
Vol 30 (12) ◽  
pp. e3786 ◽  
Author(s):  
Charlie Y. Wang ◽  
Yuchi Liu ◽  
Shuying Huang ◽  
Mark A. Griswold ◽  
Nicole Seiberlich ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Magnetic Resonance ◽  
Reaction Rate ◽  
Kinase Reaction ◽  
Creatine Kinase Reaction ◽  
Rapid Quantification

Phosphocreatine content of freeze-clamped muscle: influence of creatine kinase inhibition

2003 ◽  
Vol 94 (5) ◽  
pp. 1751-1756 ◽  
Author(s):  
Jeffrey J. Brault ◽  
Kirk A. Abraham ◽  
Ronald L. Terjung
Keyword(s):  
Creatine Kinase ◽  
Kinase Inhibitor ◽  
Fiber Type ◽  
High Energy ◽  
High Energy Phosphates ◽  
Kinase Inhibition ◽  
Chemical Measurements ◽  
Cellular Energetics ◽  
Muscle Energetic

The study of cellular energetics is critically dependent on accurate measurement of high-energy phosphates. Muscle values of phosphocreatine (PCr) vary greatly between in vivo measurements (i.e., by nuclear magnetic resonance) and chemical measurements determined from muscles isolated and quick-frozen. The source of this difference has not been experimentally identified. A likely cause is activation of ATPases and phosphotransfer from PCr to ADP. Therefore, rat hindlimb skeletal muscle was perfused either with or without 2 mM iodoacetamide, a creatine kinase inhibitor, and muscle was freeze-clamped either at rest or after contraction. Creatine kinase inhibition resulted in ∼6 μmol/g higher PCr and lower creatine in the freeze-clamped soleus, red gastrocnemius, and white gastrocnemius. This PCr content difference was reduced when the initial PCr content was decreased with prior contractions. Therefore, the amount of PCr artifact appears to scale with initial PCr content within a fiber-type section. This artifact directly affects the measurement and, thus, the calculations of muscle energetic parameters from studies using isolated and frozen muscle.

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