scholarly journals Silencing of the glycerophosphocholine phosphodiesterase GDPD5 alters the phospholipid metabolite profile in a breast cancer modelin vivoas monitored by31P MRS

2014 ◽  
Vol 27 (6) ◽  
pp. 692-699 ◽  
Author(s):  
J. P. Wijnen ◽  
L. Jiang ◽  
T. R. Greenwood ◽  
M. Cheng ◽  
M. Döpkens ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metabolite Profile ◽  
Glycerophosphocholine Phosphodiesterase

GDPD5 inhibition alters the choline phospholipid metabolite profile of breast cancer cells toward a less malignant metabolic profile

2012 ◽  
Vol 1 (1) ◽  
pp. 3-15
Author(s):  
Mailin Döpkens ◽  
Tiffany R. Greenwood ◽  
Farhad Vesuna ◽  
Venu Raman ◽  
Dieter Leibfritz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Metabolic Profile ◽  
Breast Cancer Cells ◽  
Metabolite Profile ◽  
Choline Phospholipid

Evaluating the Metabolite Profile of HER2‐positive Breast Cancer Cells

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Sarah Abuelreich ◽  
Nicole Leon ◽  
Daisy Medina ◽  
Malika Sahni ◽  
Krish Krishnan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Metabolite Profile ◽  
Her2 Positive ◽  
Her2 Positive Breast Cancer ◽  
Positive Breast Cancer

scholarly journals Untargeted Urinary 1H NMR-Based Metabolomic Pattern as a Potential Platform in Breast Cancer Detection

Metabolites ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 269 ◽  
Author(s):  
Catarina L. Silva ◽  
Ana Olival ◽  
Rosa Perestrelo ◽  
Pedro Silva ◽  
Helena Tomás ◽  
...  
Keyword(s):  
Breast Cancer ◽  
1H Nmr ◽  
Biological Fluids ◽  
Characteristic Curve ◽  
Metabolite Profile ◽  
Medical Diagnostics ◽  
Data Matrix ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
Healthy Controls

Breast cancer (BC) remains the second leading cause of death among women worldwide. An emerging approach based on the identification of endogenous metabolites (EMs) and the establishment of the metabolomic fingerprint of biological fluids constitutes a new frontier in medical diagnostics and a promising strategy to differentiate cancer patients from healthy individuals. In this work we aimed to establish the urinary metabolomic patterns from 40 BC patients and 38 healthy controls (CTL) using proton nuclear magnetic resonance spectroscopy (1H-NMR) as a powerful approach to identify a set of BC-specific metabolites which might be employed in the diagnosis of BC. Orthogonal partial least squares-discriminant analysis (OPLS-DA) was applied to a 1H-NMR processed data matrix. Metabolomic patterns distinguished BC from CTL urine samples, suggesting a unique metabolite profile for each investigated group. A total of 10 metabolites exhibited the highest contribution towards discriminating BC patients from healthy controls (variable importance in projection (VIP) >1, p < 0.05). The discrimination efficiency and accuracy of the urinary EMs were ascertained by receiver operating characteristic curve (ROC) analysis that allowed the identification of some metabolites with the highest sensitivities and specificities to discriminate BC patients from healthy controls (e.g. creatine, glycine, trimethylamine N-oxide, and serine). The metabolomic pathway analysis indicated several metabolism pathway disruptions, including amino acid and carbohydrate metabolisms, in BC patients, namely, glycine and butanoate metabolisms. The obtained results support the high throughput potential of NMR-based urinary metabolomics patterns in discriminating BC patients from CTL. Further investigations could unravel novel mechanistic insights into disease pathophysiology, monitor disease recurrence, and predict patient response towards therapy.

scholarly journals Vitamin D Metabolite Profile in Cholecalciferol- or Calcitriol-Supplemented Healthy and Mammary Gland Tumor-Bearing Mice

Nutrients ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3416
Author(s):  
Artur Anisiewicz ◽  
Konrad Kowalski ◽  
Joanna Banach ◽  
Natalia Łabędź ◽  
Martyna Stachowicz-Suhs ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Vitamin D ◽  
Tumor Growth ◽  
Vitamin D3 ◽  
Metabolite Profile ◽  
Tumor Burden ◽  
Breast Cancers ◽  
Tumor Bearing ◽  
4T1 Breast Cancer ◽  
The Impact

To analyze if the prometastatic activity of calcitriol (active vitamin D3 metabolite), which was previously observed in a 4T1 breast cancer model, is also found in other breast cancers, and to assess the impact of various schemes of vitamin D supply, we used 4T1 and E0771 mouse metastatic and 67NR nonmetastatic cells in this study. BALB/c and C57BL/6 healthy and tumor-bearing mice were exposed to a control (1000 IU), low- (100 IU), and high- (5000 IU) vitamin D3 diets. Additionally, from day 7 of tumor transplantation, the 1000 and 100 IU groups were gavaged with calcitriol (+cal). After 8 weeks of feeding, plasma levels of 25(OH)D3, 24,25(OH)2D3, and 3-epi-25(OH)D3 were significantly lower in calcitriol-treated and vitamin D-deficient groups than in the control, whereas the levels of all metabolites were increased in the 5000 IU group. The ratio of 25(OH)D3:24,25(OH)2D3 was increased in both calcitriol-treated groups, whereas the ratio of 25(OH)D3:3-epi-25(OH)D3 was increased only in the 100 IU group but decreased in the 5000 IU group. In contrast to E0771, 4T1 lung metastasis was accelerated in all vitamin D-supplemented mice, as well as in the deficient group with an increased inflammatory response. 67NR tumor growth was transiently inhibited in the 1000 IU+cal group, but single metastases were observed in the 5000 and 100 IU groups. Based on the results, we conclude that various schemes of vitamin D supply and vitamin D deficiency led to similar metabolite profiles irrespective of the mice strain and tumor burden. However, depending on the type of breast cancer, different effects on tumor growth and metastasis were noticed.

Development and initial validation of a metabolite profile for the early detection of breast cancer recurrence.

2012 ◽  
Vol 30 (30_suppl) ◽  
pp. 5-5
Author(s):  
Daniel Raftery ◽  
Haiwei Gu ◽  
Vincent Asiago ◽  
Leiddy Alvarado ◽  
Danijel Djukovic ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Early Detection ◽  
Cross Validation ◽  
Metabolite Profile ◽  
Recurrent Breast Cancer ◽  
Labeled Compounds ◽  
Initial Validation ◽  
Recurrent Breast ◽  
Fold Cross Validation ◽  
Metabolite Markers

5 Background: The detection of recurrent breast cancer is limited by poorly performing CA markers that are both insensitive and late markers. Because of their sensitivity to biological status, metabolite markers may provide better diagnostic performance and earlier detection. Detectable perturbations in the metabolic profiles of patients often result from altered metabolism in cancer. We report on the discovery and initial validation of a metabolite profile for the early detection of recurrent breast cancer. Methods: We applied a combination of nuclear magnetic resonance (NMR) and gas chromatography-mass spectrometry (GC-MS) to analyze the metabolite profiles of 116 serial serum samples from 20 recurring patients and 141 serial samples from 36 breast cancer survivors with no evidence of disease (NED). Multivariate analysis was used to identify 11 metabolite markers that were used to build a model with high accuracy (AUROC >0.88 using 10 fold cross validation) with a sensitivity of 68% and specificity of 94%. Strikingly, over 55% of the patients could be correctly predicted to have recurrence on average 13 months before clinical diagnosis, representing a large improvement over the current diagnostic assays CA 27.29 and CA 15-3 (Cancer Res. 2010; 70, 8309-18). The metabolites were then ported to an LC-MS/MS platform and a method was developed to quantify these metabolites using stable isotope labeled compounds. Due to difficulty in obtaining some labeled compounds the profile was reduced to 9 metabolites. The multivariate algorithm was recalculating using five-fold cross validation and using 211 patient samples. During this work we found that the performance of the assay could be improved by adding CA27-29 values to the assay, which mainly ensured excellent specificity. Results: The profile was tested using a separate validation set of 96 patient samples run identically. The performance was similar to the training set with a sensitivity of 65% and specificity of 93%. Recurrence detection was approximately 11 months ahead of clinical diagnosis (based on imaging for symptomatic patients) and about 2 years ahead of CA 27-29 alone. Conclusions: This metabolite profile has promise for early recurrence detection.

Virus-Like Particles in a Human Breast Cancer: Structural Similarity to Previously Reported Particles

1973 ◽  
Vol 31 ◽  
pp. 378-379
Author(s):  
G. Kasnic ◽  
S. E. Stewart ◽  
C. Urbanski
Keyword(s):  
Breast Cancer ◽  
Biological Activity ◽  
Human Breast Cancer ◽  
Osteogenic Sarcoma ◽  
Human Tumor ◽  
Structural Similarity ◽  
Cell Tumor ◽  
Human Breast ◽  
Human Tumor Cell ◽  
Type Particle

We have reported the maturation of an intracisternal A-type particle in murine plasma cell tumor cultures and three human tumor cell cultures (rhabdomyosarcoma, lung adenocarcinoma, and osteogenic sarcoma) after IUDR-DMSO activation. In all of these studies the A-type particle seems to develop into a form with an electron dense nucleoid, presumably mature, which is also intracisternal. A similar intracisternal A-type particle has been described in leukemic guinea pigs. Although no biological activity has yet been demonstrated for these particles, on morphologic grounds, and by the manner in which they develop within the cell, they may represent members of the same family of viruses.

Further Ultrastructural Observations on Metastatic Nodules of Human Breast Cancer

1968 ◽  
Vol 26 ◽  
pp. 224-225
Author(s):  
John L. Swedo ◽  
R. W. Talley ◽  
John H. L. Watson
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Estrogen Therapy ◽  
Specimen Preparation ◽  
Human Breast ◽  
Autophagic Vacuoles ◽  
Previous Communication ◽  
Linear Profiles ◽  
Metastatic Nodule

Since the report, which described the ultrastructure of a metastatic nodule of human breast cancer after estrogen therapy, additional ultrastructural observations, including some which are correlative with pertinent findings in the literature concerning mycoplasmas, have been recorded concerning the same subject. Specimen preparation was identical to that in.The mitochondria possessed few cristae, and were deteriorated and vacuolated. They often contained particulates and fibrous structures, sometimes arranged in spindle-shaped bundles, Fig. 1. Another apparent aberration was the occurrence, Fig. 2 (arrows) of linear profiles of what seems to be SER, which lie between layers of RER, and are often recognizably continuous with them.It was noted that the structure of the round bodies, interpreted as within autophagic vacuoles in the previous communication, and of vesicular bodies, described morphologically closely resembled those of some mycoplasmas. Specifically, they simulated or reflected the various stages of replication reported for mycoplasmas grown on solid nutrient. Based on this observation, they are referred to here as “mycoplasma-like” structures, in anticipation of confirmatory evidence from investigations now in progress.

The proliferation of breast cancer cells are inhibited by the human intrabody targeting cyclinD1

2010 ◽  
Vol 34 (8) ◽  
pp. S49-S49
Author(s):  
Lei Wang ◽  
Xun Zhou ◽  
Lihong Zhou ◽  
Yong Chen ◽  
Xun Zhu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells

Study of the mechanism responsible for multidrug resistance in breast cancer MCF-7 cell mediated by 14-3-3σ

2010 ◽  
Vol 34 (8) ◽  
pp. S47-S47
Author(s):  
Guopei Zheng ◽  
Sisi Yi ◽  
Yafei Li ◽  
Fangren Kong ◽  
Yanhui Yu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Multidrug Resistance ◽  
Mcf 7
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