Estrogen inhibits bladder overactivity in rats with cyclophosphamide‐induced cystitis via downregulating the expression of P2X3 receptors in bladder epithelium cells

2021 ◽  
Author(s):  
Xin Liu ◽  
Yang Yang ◽  
Ye Gao ◽  
Hengshuai Zhang ◽  
Jingzhen Zhu ◽  
...  
Keyword(s):  
Bladder Epithelium ◽  
P2x3 Receptors ◽  
Epithelium Cells

Cell surface changes during aging of human bladder epithelium examined by lectin probes and SEM

1990 ◽  
Vol 48 (3) ◽  
pp. 966-967
Author(s):  
J. Jacob ◽  
M.F.M. Ismail
Keyword(s):  
The Elderly ◽  
Ultrastructural Changes ◽  
Pokeweed Mitogen ◽  
Asymmetric Structure ◽  
Luminal Surface ◽  
Bladder Epithelium ◽  
Aldehyde Groups ◽  
Cell Surface Changes ◽  
Phosphate Buffered Saline ◽  
Surface Changes

Ultrastructural changes have been shown to occur in the urinary bladder epithelium (urothelium) during the life span of humans. With increasing age, the luminal surface becomes more flexible and develops simple microvilli-like processes. Furthermore, the specialised asymmetric structure of the luminal plasma membrane is relatively more prominent in the young than in the elderly. The nature of the changes at the luminal surface is now explored by lectin-mediated adsorption visualised by scanning electron microscopy (SEM).Samples of young adult (21-31 y old) and elderly (58-82 y old) urothelia were fixed in buffered 2% glutaraldehyde for 10 m and washed with phosphate buffered saline (PBS) containing Ca++ and Mg++ at room temperature. They were incubated overnight at 4°C in 0.1 M ammonium chloride in PBS to block any remaining aldehyde groups. The samples were then allowed to stand in PBS at 37°C for 2 h before incubation at 37°C for 30 m with lectins. The lectins used were concanavalin A (Con A), wheat germ agglutinin (WGA), phytohaemagglutinin (PHA) and pokeweed mitogen (PWM) at a concentration of 500 mg/ml in PBS at pH 7.A.

299: The Expressions of Multiple Angiogenic Factors in Bladder Epithelium in Patients with Refractory Overactive Bladder

2005 ◽  
Vol 173 (4S) ◽  
pp. 82-83
Author(s):  
Masahiro Tamaki ◽  
Tadashi Hayashi ◽  
Osamu Ogawa ◽  
Tomohiro Ueda ◽  
Naoki Yoshimura
Keyword(s):  
Overactive Bladder ◽  
Angiogenic Factors ◽  
Bladder Epithelium

Initial effects of cyclophosphamide on urinary bladder epithelium in the rat

Pathology ◽  
1974 ◽  
Vol 6 (4) ◽  
pp. 343-350 ◽  
Author(s):  
Mary E. Schultz ◽  
Michael W. Weldon
Keyword(s):  
Urinary Bladder ◽  
Bladder Epithelium

Формирование иммунного статуса у новорожденных телят молочных и комбинированных пород

2019 ◽  
pp. 32-37
Author(s):  
L.N. BAKAEVA ◽  
A.S. KARAMAEVA ◽  
S.V. KARAMAEV
Keyword(s):  
Blood Serum ◽  
Holstein Cows ◽  
Morbidity Rate ◽  
Immune Development ◽  
Black And White ◽  
Alveolar Tissue ◽  
Holstein Calves ◽  
Newborn Calves ◽  
Middle Volga ◽  
Epithelium Cells

Работа  посвящена изучению особенностей формирования иммунитета у новорожденных телят. Объектом исследований служили коровы бестужевской, черно-пестрой, голштинской, айрширской пород и их потомство, разводимые в природно-климатической зоне Среднего Поволжья и Южного Урала. Установлено, что за последний месяц перед отелом в сыворотке крови коров происходит снижение содержания иммуноглобулинов, у бестужевской породы на 19,3%, черно-пестрой — на 15,8%, голштинской — на 13,4%, айрширской — на 15,0%. Это обусловлено тем, что иммуноглобулины интенсивно перемещаются в клетки секреторного эпителия альвеолярной ткани вымени. В молозиве первого удоя содержится от 57,86 г/л (голштинская порода) до 106,78 г/л (бестужевская порода) иммуноглобулинов. В молозиве второго удоя содержание иммуноглобулинов снижается у бестужевской породы на 18,9%, черно-пестрой — на 25,1%, голштинской — на 29,3%, айрширской — на 21,7%, а при четвертом доении, соответственно, на 45,4; 51,6; 52,4; 49,2%. Через 6 ч после первого выпаивания молозивом, содержание иммуноглобулинов в сыворотке крови телят всех пород, за исключением голштинской, было выше 10 мг/мл, что считается физиологической нормой. Исследования показали, что в каждой породе есть телята, не воспринимающие иммуноглобулины из молозива матери, доля которых составляет, соответственно 20; 16; 30; 10%. Наиболее опасными для здоровья теленка являются первые 5 дней жизни. В этот период в бестужевской породе заболело 6% телят, черно-пестрой — 24, голштинской — 42, айрширской — 14%. Всего за первый месяц жизни заболеваемость телят в группах составила, соответственно, 16; 48; 76; 26%.The article presents the study of peculiarities of immune development in newborn calves. The objects of interest were Bestuzhev, Black-and-White, Holstein and Ayrshire cows and their progeny raised in the environmental and climatic zone of Middle Volga and Southern Ural. The study has shown that during the last month prior to calving there was a decrease of immunoglobulin content in the cows’ blood serum: in Bestuzhev cows by 19.3%, in Black-and-White cows by 15.8%, in Holstein cows by 13.4% and in Ayrshire cows by 15.0%. This is due to the fact that immunoglobulins massively migrate to secretory epithelium cells of the udder alveolar tissue. Immunoglobulin concentration in the first milking colostrum was from 57.86 g/l (Holstein cows) up to 106.78 g/l (Bestuzhev cows). In the second milking colostrum the content of immunoglobulins was decreased by 18.9% in Bestuzhev cows, by 25.1% in Black-and-White cows, by 29.3% in Holstein cows and by 21.7% in Ayrshire cows, in the forth milking colostrum the decrease was respectively by 45.4; 51.6; 52.4 and 49.2%. 6 hours after the first colostrum feeding immunoglobulin content in blood serum of calves of all breeds except for Holstein one exceeded 10 mg/ml, what was within the physiological norm. The study has shown that there were calves in every breed who did not accept immunoglobulins from the mother’s colostrum, their percentage was respectively 20; 16; 30 and 10%. The first five days of life were the most dangerous for the health of calves. During the period 6% Bestuzhev calves, 24% Black-and-White calves, 42 Holstein calves and 14% Ayrshire calves fell ill. During the fist month of life the morbidity rate of calves was respectively 16; 48; 76 and 26%.

Calcium transport in turtle bladder

1987 ◽  
Vol 253 (6) ◽  
pp. R917-R921
Author(s):  
S. Sabatini ◽  
N. A. Kurtzman
Keyword(s):  
Calcium Transport ◽  
Calcium Absorption ◽  
Short Circuit ◽  
Open Circuit ◽  
Calcium Flux ◽  
Bladder Epithelium ◽  
Control Value ◽  
Sodium Calcium ◽  
Turtle Bladder ◽  
Absorptive Flux

Unidirectional 45Ca fluxes were measured in the turtle bladder under open-circuit and short-circuit conditions. In the open-circuited state net calcium flux (JnetCa) was secretory (serosa to mucosa) and was 388.3 +/- 84.5 pmol.mg-1.h-1 (n = 20, P less than 0.001). Ouabain (5 X 10(-4) M) reversed JnetCa to an absorptive flux (serosal minus mucosal flux = -195.8 +/- 41.3 pmol.mg-1.h-1; n = 20, P less than 0.001). Amiloride (1 X 10(-5) M) reduced both fluxes such that JnetCa was not significantly different from zero. Removal of mucosal sodium caused net calcium absorption; removal of serosal sodium caused calcium secretion. When bladders were short circuited, JnetCa decreased to approximately one-third of control value but remained secretory (138.4 +/- 54.3 pmol.mg-1.h-1; n = 9, P less than 0.025). When ouabain was added under short-circuit conditions, JnetCa was similar in magnitude and direction to ouabain under open-circuited conditions (i.e., absorptive). Tissue 45Ca content was approximately equal to 30-fold lower when the isotope was placed in the mucosal bath, suggesting that the apical membrane is the resistance barrier to calcium transport. The results obtained in this study are best explained by postulating a Ca2+-ATPase on the serosa of the turtle bladder epithelium and a sodium-calcium antiporter on the mucosa. In this model, the energy for calcium movement would be supplied, in large part, by the Na+-K+-ATPase. By increasing cell sodium, ouabain would decrease the activity of the mucosal sodium-calcium exchanger (or reverse it), uncovering active calcium transport across the serosa.

scholarly journals An infection-induced RhoB-Beclin 1-Hsp90 complex enhances clearance of uropathogenic Escherichia coli

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Chunhui Miao ◽  
Mingyu Yu ◽  
Geng Pei ◽  
Zhenyi Ma ◽  
Lisong Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Treatment Strategies ◽  
Uropathogenic Escherichia Coli ◽  
Beclin 1 ◽  
Host Cells ◽  
Infection Model ◽  
Intracellular Bacteria ◽  
Bladder Epithelium ◽  
Binding Residue

AbstractHost cells use several anti-bacterial pathways to defend against pathogens. Here, using a uropathogenic Escherichia coli (UPEC) infection model, we demonstrate that bacterial infection upregulates RhoB, which subsequently promotes intracellular bacteria clearance by inducing LC3 lipidation and autophagosome formation. RhoB binds with Beclin 1 through its residues at 118 to 140 and the Beclin 1 CCD domain, with RhoB Arg133 being the key binding residue. Binding of RhoB to Beclin 1 enhances the Hsp90-Beclin 1 interaction, preventing Beclin 1 degradation. RhoB also directly interacts with Hsp90, maintaining RhoB levels. UPEC infections increase RhoB, Beclin 1 and LC3 levels in bladder epithelium in vivo, whereas Beclin 1 and LC3 levels as well as UPEC clearance are substantially reduced in RhoB+/− and RhoB−/− mice upon infection. We conclude that when stimulated by UPEC infections, host cells promote UPEC clearance through the RhoB-Beclin 1-HSP90 complex, indicating RhoB may be a useful target when developing UPEC treatment strategies.

Surface Labeling with Adhesion Protein FimH Improves Binding of Immunotherapeutic Agent Salmonella Ty21a to the Bladder Epithelium

2020 ◽  
pp. 1-12
Author(s):  
Maroeska J. Burggraaf ◽  
Lisette Waanders ◽  
Mariska Verlaan ◽  
Janneke Maaskant ◽  
Diane Houben ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Antitumor Activity ◽  
Bladder Wall ◽  
Bladder Epithelium ◽  
Modest Improvement ◽  
Adhesion Protein ◽  
Survival Experiment ◽  
Muscle Invasive

BACKGROUND: Bladder cancer is the ninth most common cancer in men. 70% of these tumors are classified as non-muscle invasive bladder cancer and those patients receive 6 intravesical instillations with Mycobacterium bovis BCG after transurethral resection. However, 30% of patients show recurrences after treatment and experience severe side effects that often lead to therapy discontinuation. Recently, another vaccine strain, Salmonella enterica typhi Ty21a, demonstrated promising antitumor activity in vivo. Here we focus on increasing bacterial retention in the bladder in order to reduce the number of instillations required and improve antitumor activity. OBJECTIVE: To increase the binding of Ty21a to the bladder wall by surface labeling of the bacteria with adhesion protein FimH and to study its effect in a bladder cancer mouse model. METHODS: Binding of Ty21a with surface-labeled FimH to the bladder wall was analyzed in vitro and in vivo. The antitumor effect of a single instillation of Ty21a+FimH in treatment was determined in a survival experiment. RESULTS: FimH-labeled Ty21a showed significant (p <  0.0001) improved binding to mouse and human cell lines in vitro. Furthermore, FimH labeled bacteria showed ∼5x more binding to the bladder than controls in vivo. Enhanced binding to the bladder via FimH labeling induced a modest improvement in median but not in overall mice survival. CONCLUSIONS: FimH labeling of Ty21a significantly improved binding to bladder tumor cells in vitro and the bladder wall in vivo. The improved binding leads to a modest increase in median survival in a single bladder cancer mouse study.

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