The extracellular matrix molecules versican and hyaluronan in urethral and vaginal tissues in stress urinary incontinence

2021 ◽  
Vol 40 (3) ◽  
pp. 771-782
Author(s):  
Ingrid A. Harten ◽  
Stephen P. Evanko ◽  
Chong H. Choe ◽  
Eugene W. Lee ◽  
Bhavin N. Patel ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Extracellular Matrix Molecules

scholarly journals An In Vitro Study on Extracellular Vesicles From Adipose-Derived Mesenchymal Stem Cells in Protecting Stress Urinary Incontinence Through MicroRNA-93/F3 Axis

2021 ◽  
Vol 12 ◽  
Author(s):  
Lu Wang ◽  
Yali Wang ◽  
Yuancui Xiang ◽  
Jinping Ma ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Extracellular Vesicles ◽  
Satellite Cells ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Collagen Iii ◽  
Primary Fibroblasts

Since the potential roles of extracellular vesicles secreted by adipose-derived mesenchymal stem cells (ADSCs) are not well understood in collagen metabolism, the purpose of this research was to evaluate the effects of ADSCs-extracellular vesicles in stress urinary incontinence and the regulatory mechanism of delivered microRNA-93 (miR-93). ADSCs were isolated and cultured, and ADSCs-extracellular vesicles were extracted and identified. Stress urinary incontinence primary fibroblasts or satellite cells were treated with ADSCs-extracellular vesicles to detect the expression of Elastin, Collagen I, and Collagen III in fibroblasts and Pax7 and MyoD in satellite cells. After transfecting ADSCs with miR-93 mimics or inhibitors, extracellular vesicles were isolated and treated with stress urinary incontinence primary fibroblasts or satellite cells to observe cell function changes. The online prediction and luciferase activity assay confirmed the targeting relationship between miR-93 and coagulation factor III (F3). The rescue experiment verified the role of ADSCs-extracellular vesicles carrying miR-93 in stress urinary incontinence primary fibroblasts and satellite cells by targeting F3. ADSCs-extracellular vesicles treatment upregulated expression of Elastin, Collagen I, and Collagen III in stress urinary incontinence primary fibroblasts and expression of Pax7 and MyoD in stress urinary incontinence primary satellite cells. miR-93 expression was increased in stress urinary incontinence primary fibroblasts or satellite cells treated with ADSCs-extracellular vesicles. Extracellular vesicles secreted by ADSCs could deliver miR-93 to fibroblasts and then negatively regulate F3 expression; ADSCs-extracellular vesicles could reverse the effect of F3 on extracellular matrix remodeling in stress urinary incontinence fibroblasts. miR-93 expression was also increased in stress urinary incontinence primary satellite cells treated by ADSCs-extracellular vesicles. Extracellular vesicles secreted by ADSCs were delivered to satellite cells through miR-93, which directly targets F3 expression and upregulates Pax7 and MyoD expression in satellite cells. Our study indicates that miR-93 delivered by ADSCs-extracellular vesicles could regulate extracellular matrix remodeling of stress urinary incontinence fibroblasts and promote activation of stress urinary incontinence satellite cells through targeting F3.

Use of bioactive extracellular matrix fragments as a urethral bulking agent to treat stress urinary incontinence

2020 ◽  
Vol 117 ◽  
pp. 156-166
Author(s):  
Ying Wang ◽  
Meng Duan ◽  
Mahbubur Rahman ◽  
Ming Yang ◽  
Weixin Zhao ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Bulking Agent ◽  
Urethral Bulking ◽  
Urethral Bulking Agent

1375: Contributions of Collagen and Elastin to Urethral Biomechanics: Correlation of Damaged Elastin to Stress Urinary Incontinence Induced by Birth Trauma

2007 ◽  
Vol 177 (4S) ◽  
pp. 453-454
Author(s):  
Rachelle L. Prantif ◽  
William C. de Groat ◽  
Donna J. Haworth ◽  
Ronald J. Jankowski ◽  
Michael B. Chancellor ◽  
...  
Keyword(s):  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Birth Trauma

337: Monarc™ Transobturator Sling for the Treatment of Stress Urinary Incontinence: A Prospective, Multi­Center Study with One Year Follow-Up

2006 ◽  
Vol 175 (4S) ◽  
pp. 110-110 ◽  
Author(s):  
Robert D. Moore ◽  
John Miklos ◽  
L. Dean Knoll ◽  
Mary Dupont ◽  
Mickey Karram ◽  
...  
Keyword(s):  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Transobturator Sling ◽  
One Year

1184: The New TVT Obturator Procedure for Stress Urinary Incontinence-Why Change a “Winning Horse”?

2006 ◽  
Vol 175 (4S) ◽  
pp. 380-380
Author(s):  
Asnat Groutz ◽  
Ronen Gold ◽  
David Pauzner ◽  
Joseph Lessing ◽  
David Gordon
Keyword(s):  
Urinary Incontinence ◽  
Stress Urinary Incontinence

494: International Multi-Center Study Evaluating the Adjustable Continence Therapy (ProACT™) for Male Post Prostatectomy Stress Urinary Incontinence

2004 ◽  
Vol 171 (4S) ◽  
pp. 132-132 ◽  
Author(s):  
Peter J. Gilling ◽  
Wilhelm A. Huebner ◽  
Flavio T. Rocha ◽  
Marcus V. Sadi ◽  
Oliver M. Schlarp
Keyword(s):  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Multi Center Study ◽  
Center Study
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